First quantitative detection of tomato brown rugose fruit virus in wastewater in Louisiana.

Tomato brown rugose fruit virus Viral indicator Wastewater qPCR

Journal

The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500

Informations de publication

Date de publication:
25 Aug 2023
Historique:
received: 25 02 2023
revised: 03 05 2023
accepted: 04 05 2023
medline: 14 6 2023
pubmed: 12 5 2023
entrez: 11 5 2023
Statut: ppublish

Résumé

We investigated the occurrence of tomato brown rugose fruit virus (ToBRFV) at a conventional wastewater treatment plant in Louisiana over a 13-month period, from March 2017 to March 2018. Influent, secondary effluent, and final effluent wastewater samples were collected monthly, and viruses were concentrated by the adsorption-elution method using an electronegative filter, followed by the detection using quantitative polymerase chain reaction. ToBRFV was detected in 10 (77 %) of 13 influent samples, 9 (69 %) of 13 in secondary effluent, and 6 (50 %) of 12 final effluents. The concentrations of ToBRFV in the influent samples ranged from 3.5 to 6.1 log

Identifiants

pubmed: 37169194
pii: S0048-9697(23)02622-0
doi: 10.1016/j.scitotenv.2023.164001
pii:
doi:

Substances chimiques

Wastewater 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

164001

Informations de copyright

Copyright © 2023. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors do not have any conflict of interest.

Auteurs

Samendra P Sherchan (SP)

Department of Environmental Health, Tulane University, New Orleans, LA 70112, United States; BioEnvironmental Science Program, Morgan State University, Baltimore, MD 21251, United States; Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan. Electronic address: sshercha@tulane.edu.

Bikash Malla (B)

Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.

Eiji Haramoto (E)

Interdisciplinary Center for River Basin Environment, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan. Electronic address: eharamoto@yamanashi.ac.jp.

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Classifications MeSH