Initial Characterization of 3D Culture of Yolk Sac Tissue.

Hanging-Drop Matrigel bovine canine porcine stem cell

Journal

Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614

Informations de publication

Date de publication:
22 Apr 2023
Historique:
received: 06 03 2023
revised: 07 04 2023
accepted: 17 04 2023
medline: 13 5 2023
pubmed: 13 5 2023
entrez: 13 5 2023
Statut: epublish

Résumé

The role of the yolk sac (YS) in miscarriage is not yet clear, largely due to ethical reasons that make in vivo studies difficult to conduct. However, 3D cultures could provide a solution to this problem by enabling cells to be arranged in a way that more closely mimics the structure of the YS as it exists in vivo. In this study, three domestic species (porcine, canine, and bovine) were chosen as models to standardize 3D culture techniques for the YS. Two techniques of 3D culture were chosen: the Matrigel

Identifiants

pubmed: 37174472
pii: ani13091435
doi: 10.3390/ani13091435
pmc: PMC10177165
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : São Paulo Research Foundation
ID : 18/24273-0

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Auteurs

Vitória Mattos Pereira (VM)

Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo (USP), Pirassununga 13635-900, SP, Brazil.
School of Pharmacy, University of Wyoming, Laramie, WY 82072, USA.

Priscila Avelino Ferreira Pinto (PAF)

Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo (USP), Pirassununga 13635-900, SP, Brazil.

Lina Castelo Branco Motta (LCB)

Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo (USP), Pirassununga 13635-900, SP, Brazil.

Matheus F Almeida (MF)

Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo (USP), Pirassununga 13635-900, SP, Brazil.

André Furugen Cesar de Andrade (AFC)

Department of Animal Reproduction, School of Veterinary Medicine and Animal Science (FMVZ), University of São Paulo USP, Pirassununga 13635-900, SP, Brazil.

Ana Paula Pinoti Pavaneli (APP)

Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo (USP), Pirassununga 13635-900, SP, Brazil.

Carlos Eduardo Ambrósio (CE)

Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo (USP), Pirassununga 13635-900, SP, Brazil.

Classifications MeSH