Persistently Elevated HBV Viral-Host Junction DNA in Urine as a Biomarker for Hepatocellular Carcinoma Minimum Residual Disease and Recurrence: A Pilot Study.
HBV integration
hepatocellular carcinoma
minimal residual disease
recurrence monitoring
urine cell-free DNA
Journal
Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402
Informations de publication
Date de publication:
25 Apr 2023
25 Apr 2023
Historique:
received:
13
02
2023
revised:
02
03
2023
accepted:
21
03
2023
medline:
13
5
2023
pubmed:
13
5
2023
entrez:
13
5
2023
Statut:
epublish
Résumé
Hepatitis B virus (HBV)-host junction sequences (HBV-JSs) has been detected in the urine of patients with HBV infection. This study evaluated HBV-JSs as a marker of minimum residual disease (MRD) and tumor recurrence after treatment in HBV-hepatocellular carcinoma (HCC) patients. Archived serial urine DNA from two HBV-HCC with recurrence as confirmed by MRI and four HBV-related cirrhosis (LC) patients were used. Urinary HBV-JSs were identified by an HBV-targeted NGS assay. Quantitative junction-specific PCR assays were developed to investigate dynamic changes of the most abundant urinary HBV-JS. Abundant urinary HBV-JSs were identified in two cases of tumor recurrence. In case 1, a 78-year-old female with HBV- HCC underwent a follow-up MRI following microwave ablation. While MRI results were variable, the unique HBV-JS DNA, HBV-Chr17, steadily increased from initial diagnosis to HCC recurrence. In case 2, a 74-year-old male with HBV-HCC contained two HBV-JS DNA, HBV-Chr11 and HBV-
Identifiants
pubmed: 37174929
pii: diagnostics13091537
doi: 10.3390/diagnostics13091537
pmc: PMC10177231
pii:
doi:
Types de publication
Case Reports
Langues
eng
Subventions
Organisme : NCI NIH HHS
ID : R01 CA202769
Pays : United States
Organisme : NCI NIH HHS
ID : R44 CA165312
Pays : United States
Organisme : NCI NIH HHS
ID : RO1 CA202769
Pays : United States
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