On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan-DNA Polyelectrolyte Complexes.

AF4 DNA SEC chitosan polyelectrolyte complex

Journal

Polymers
ISSN: 2073-4360
Titre abrégé: Polymers (Basel)
Pays: Switzerland
ID NLM: 101545357

Informations de publication

Date de publication:
28 Apr 2023
Historique:
received: 30 11 2022
revised: 11 04 2023
accepted: 24 04 2023
medline: 13 5 2023
pubmed: 13 5 2023
entrez: 13 5 2023
Statut: epublish

Résumé

Chitosan is extensively studied as a carrier for gene delivery and is an attractive non-viral gene vector owing to its polycationic, biodegradable, and biocompatible nature. Thus, it is essential to understand the chemistry of self-assembled chitosan-DNA complexation and their structural and functional properties, enabling the formation of an effective non-viral gene delivery system. In this study, two parent chitosans (samples NAS-032 and NAS-075; Mw range ~118-164 kDa) and their depolymerised derivatives (deploy nas-032 and deploy nas-075; Mw range 6-14 kDa) with degrees of acetylation 43.4 and 4.7%, respectively, were used to form polyelectrolyte complexes (PECs) with DNA at varying [-NH

Identifiants

pubmed: 37177260
pii: polym15092115
doi: 10.3390/polym15092115
pmc: PMC10180698
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Commonwealth Scholarship Commission
ID : PKCS-2019-493

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Auteurs

Ayesha Sajid (A)

School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK.

Matteo Castronovo (M)

School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK.

Francisco M Goycoolea (FM)

School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK.

Classifications MeSH