Early life nutrition affects the molecular ontogeny of testicular development in the young bull calf.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
25 04 2023
Historique:
received: 06 05 2022
accepted: 04 11 2022
medline: 17 5 2023
pubmed: 15 5 2023
entrez: 15 5 2023
Statut: epublish

Résumé

Enhanced early life nutrition accelerates sexual development in the bull calf through neuroendocrine-signalling mediated via the hypothalamic-pituitary-testicular axis. Our aim was to assess the impact of contrasting feeding regimes in bull calves during the first 12 weeks of life on the testes transcriptome and proteome. Holstein-Friesian bull calves were offered either a high (HI) or moderate (MOD) plane of nutrition, designed to support target growth rates of 1.0 and 0.5 kg/day, respectively. At 12 weeks of age all calves were euthanized, testicular parenchyma sampled, and global transcriptome (miRNAseq and mRNAseq) and proteome analyses undertaken. Bioinformatic analyses revealed 7 differentially expressed (DE) miRNA and 20 DE mRNA. There were no differentially abundant proteins between the two dietary groups. Integration of omics results highlighted a potential role for the cadherin gene, CDH13, in earlier reproductive development. Furthermore, co-regulatory network analysis of the proteomic data revealed CDH13 as a hub protein within a network enriched for processes related to insulin, IGF-1, androgen and Sertoli cell junction signalling pathways as well as cholesterol biosynthesis. Overall, results highlight a potential role for CDH13 in mediating earlier reproductive development as a consequence of enhanced early life nutrition in the bull calf.

Identifiants

pubmed: 37185277
doi: 10.1038/s41598-022-23743-3
pii: 10.1038/s41598-022-23743-3
pmc: PMC10130005
doi:

Substances chimiques

Proteome 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6748

Informations de copyright

© 2023. The Author(s).

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Auteurs

Stephen Coen (S)

Teagasc Animal and Grassland Research and Innovation Centre, Grange, Dunsany, Co. Meath, Ireland.
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.

Kate Keogh (K)

Teagasc Animal and Grassland Research and Innovation Centre, Grange, Dunsany, Co. Meath, Ireland.

Pat Lonergan (P)

School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.

Sean Fair (S)

Laboratory of Animal Reproduction, Department of Biological Sciences, University of Limerick, Limerick, Ireland.

David A Kenny (DA)

Teagasc Animal and Grassland Research and Innovation Centre, Grange, Dunsany, Co. Meath, Ireland. david.kenny@teagasc.ie.

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Classifications MeSH