How Does the Sample Preparation of
Phytophthora infestans
RNA isolation
oomycete
Journal
Current issues in molecular biology
ISSN: 1467-3045
Titre abrégé: Curr Issues Mol Biol
Pays: Switzerland
ID NLM: 100931761
Informations de publication
Date de publication:
18 Apr 2023
18 Apr 2023
Historique:
received:
28
02
2023
revised:
09
04
2023
accepted:
13
04
2023
medline:
15
5
2023
pubmed:
15
5
2023
entrez:
15
5
2023
Statut:
epublish
Résumé
RNA isolation from fungi and fungus-like organisms is not an easy task. Active endogenous RNases quickly hydrolyze RNA after the sample collection, and the thick cell wall prevents inhibitors from penetrating the cells. Therefore, the initial collection and grinding steps may be crucial for the total RNA isolation from the mycelium. When isolating RNA from
Identifiants
pubmed: 37185754
pii: cimb45040230
doi: 10.3390/cimb45040230
pmc: PMC10136579
doi:
Types de publication
Journal Article
Langues
eng
Pagination
3517-3524Subventions
Organisme : grant from the President of the Russian Federation for state support of young Russian scientists
ID : МК-4311.2022.5
Références
Anal Biochem. 2014 May 15;453:58-60
pubmed: 24607793
J Microbiol Methods. 2020 Mar;170:105855
pubmed: 32004552
Mol Biotechnol. 2008 Nov;40(3):299-305
pubmed: 18679833
BMC Genomics. 2018 Jun 19;19(1):474
pubmed: 29914352
PLoS One. 2020 Feb 12;15(2):e0227284
pubmed: 32050262
Curr Genet. 2021 Oct;67(5):823-830
pubmed: 33837814
J Microbiol Methods. 2021 May;184:106200
pubmed: 33713728
BMC Res Notes. 2013 Feb 26;6:71
pubmed: 23442734
BMC Mol Biol. 2006 Jan 31;7:3
pubmed: 16448564
J Microbiol Methods. 2012 Mar;88(3):413-8
pubmed: 22289387
Fungal Genet Biol. 2014 Nov;72:201-206
pubmed: 24951842
Mol Plant Pathol. 2008 May;9(3):385-402
pubmed: 18705878