Myogenic differentiation of human myoblasts and Mesenchymal stromal cells under GDF11 on NPoly-ɛ-caprolactone-collagen I-Polyethylene-nanofibers.
Humans
Mice
Animals
Nanofibers
Tissue Scaffolds
Polyethylene
/ metabolism
Polyesters
/ metabolism
Mesenchymal Stem Cells
/ metabolism
Myoblasts
/ metabolism
Cell Differentiation
Polyethylene Glycols
/ metabolism
Collagen
/ metabolism
Bone Morphogenetic Proteins
/ metabolism
Growth Differentiation Factors
/ metabolism
ADSC
Collagen
Electrospun composite nanofibers
GDF11
Myoblasts
Myogenic differentiation
Myostatin
Nanoscaffolds
Polyethylene oxide (PEO)
poly-ε-caprolacton (PCL)
Journal
BMC molecular and cell biology
ISSN: 2661-8850
Titre abrégé: BMC Mol Cell Biol
Pays: England
ID NLM: 101741148
Informations de publication
Date de publication:
15 May 2023
15 May 2023
Historique:
received:
19
02
2023
accepted:
27
04
2023
medline:
17
5
2023
pubmed:
16
5
2023
entrez:
15
5
2023
Statut:
epublish
Résumé
For the purpose of skeletal muscle engineering, primary myoblasts (Mb) and adipogenic mesenchymal stem cells (ADSC) can be co-cultured and myogenically differentiated. Electrospun composite nanofiber scaffolds represent suitable matrices for tissue engineering of skeletal muscle, combining both biocompatibility and stability Although growth differentiation factor 11 (GDF11) has been proposed as a rejuvenating circulating factor, restoring skeletal muscle function in aging mice, some studies have also described a harming effect of GDF11. Therefore, the aim of the study was to analyze the effect of GDF11 on co-cultures of Mb and ADSC on poly-ε-caprolactone (PCL)-collagen I-polyethylene oxide (PEO)-nanofibers. Human Mb were co-cultured with ADSC two-dimensionally (2D) as monolayers or three-dimensionally (3D) on aligned PCL-collagen I-PEO-nanofibers. Differentiation media were either serum-free with or without GDF11, or serum containing as in a conventional differentiation medium. Cell viability was higher after conventional myogenic differentiation compared to serum-free and serum-free + GDF11 differentiation as was creatine kinase activity. Immunofluorescence staining showed myosine heavy chain expression in all groups after 28 days of differentiation without any clear evidence of more or less pronounced expression in either group. Gene expression of myosine heavy chain (MYH2) increased after serum-free + GDF11 stimulation compared to serum-free stimulation alone. This is the first study analyzing the effect of GDF11 on myogenic differentiation of Mb and ADSC co-cultures under serum-free conditions. The results of this study show that PCL-collagen I-PEO-nanofibers represent a suitable matrix for 3D myogenic differentiation of Mb and ADSC. In this context, GDF11 seems to promote myogenic differentiation of Mb and ADSC co-cultures compared to serum-free differentiation without any evidence of a harming effect.
Sections du résumé
BACKGROUND
BACKGROUND
For the purpose of skeletal muscle engineering, primary myoblasts (Mb) and adipogenic mesenchymal stem cells (ADSC) can be co-cultured and myogenically differentiated. Electrospun composite nanofiber scaffolds represent suitable matrices for tissue engineering of skeletal muscle, combining both biocompatibility and stability Although growth differentiation factor 11 (GDF11) has been proposed as a rejuvenating circulating factor, restoring skeletal muscle function in aging mice, some studies have also described a harming effect of GDF11. Therefore, the aim of the study was to analyze the effect of GDF11 on co-cultures of Mb and ADSC on poly-ε-caprolactone (PCL)-collagen I-polyethylene oxide (PEO)-nanofibers.
RESULTS
RESULTS
Human Mb were co-cultured with ADSC two-dimensionally (2D) as monolayers or three-dimensionally (3D) on aligned PCL-collagen I-PEO-nanofibers. Differentiation media were either serum-free with or without GDF11, or serum containing as in a conventional differentiation medium. Cell viability was higher after conventional myogenic differentiation compared to serum-free and serum-free + GDF11 differentiation as was creatine kinase activity. Immunofluorescence staining showed myosine heavy chain expression in all groups after 28 days of differentiation without any clear evidence of more or less pronounced expression in either group. Gene expression of myosine heavy chain (MYH2) increased after serum-free + GDF11 stimulation compared to serum-free stimulation alone.
CONCLUSIONS
CONCLUSIONS
This is the first study analyzing the effect of GDF11 on myogenic differentiation of Mb and ADSC co-cultures under serum-free conditions. The results of this study show that PCL-collagen I-PEO-nanofibers represent a suitable matrix for 3D myogenic differentiation of Mb and ADSC. In this context, GDF11 seems to promote myogenic differentiation of Mb and ADSC co-cultures compared to serum-free differentiation without any evidence of a harming effect.
Identifiants
pubmed: 37189080
doi: 10.1186/s12860-023-00478-1
pii: 10.1186/s12860-023-00478-1
pmc: PMC10184409
doi:
Substances chimiques
caprolactone
56RE988L1R
Polyethylene
9002-88-4
Polyesters
0
Polyethylene Glycols
3WJQ0SDW1A
Collagen
9007-34-5
GDF11 protein, human
0
Bone Morphogenetic Proteins
0
Growth Differentiation Factors
0
Gdf11 protein, mouse
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18Informations de copyright
© 2023. The Author(s).
Références
Nature. 1997 May 1;387(6628):83-90
pubmed: 9139826
Hum Mol Genet. 2015 Nov 1;24(21):6029-40
pubmed: 26264578
Science. 2014 May 9;344(6184):649-52
pubmed: 24797481
Cytotechnology. 2020 Oct;72(5):605-614
pubmed: 32902721
Cell. 2005 Jul 29;122(2):289-301
pubmed: 16051152
Crit Rev Biochem Mol Biol. 2019 Apr;54(2):174-183
pubmed: 31144559
BMC Dev Biol. 2009 Mar 19;9:24
pubmed: 19298661
Ann N Y Acad Sci. 2019 Mar;1440(1):54-66
pubmed: 30575056
BMC Cell Biol. 2017 Feb 28;18(1):15
pubmed: 28245809
Biology (Basel). 2021 Jun 16;10(6):
pubmed: 34208436
Front Aging Neurosci. 2015 Apr 21;7:37
pubmed: 25954192
Bull Exp Biol Med. 2018 Mar;164(4):536-542
pubmed: 29504093
J Biomed Mater Res A. 2012 Sep;100(9):2302-11
pubmed: 22508579
Cell Metab. 2015 Jul 7;22(1):54-6
pubmed: 26003784
Biomaterials. 2015 Dec;73:23-31
pubmed: 26398306
Cell Biol Int. 2011 Apr;35(4):397-406
pubmed: 20946104
Nat Commun. 2016 Sep 22;7:12794
pubmed: 27653144
Basic Res Cardiol. 2017 Jul;112(4):48
pubmed: 28647906
Tissue Eng Part A. 2021 Sep;27(17-18):1151-1159
pubmed: 33203338
Cells. 2022 Nov 25;11(23):
pubmed: 36497034
Int J Mol Sci. 2020 May 07;21(9):
pubmed: 32392778
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:278-283
pubmed: 28024587
Int J Mol Sci. 2021 Sep 24;22(19):
pubmed: 34638631
J Cell Sci. 2006 Jul 15;119(Pt 14):2945-52
pubmed: 16825428
Int J Mol Sci. 2016 Aug 03;17(8):
pubmed: 27527147
Mech Ageing Dev. 2017 Jun;164:108-112
pubmed: 28472635
Front Physiol. 2014 Sep 22;5:362
pubmed: 25295011
Cell. 2013 May 9;153(4):828-39
pubmed: 23663781
Front Physiol. 2018 Feb 27;9:152
pubmed: 29535644
Skelet Muscle. 2019 May 27;9(1):16
pubmed: 31133057
Nature. 1977 Dec 22-29;270(5639):725-7
pubmed: 563524
Cells. 2022 Apr 24;11(9):
pubmed: 35563742
Biomaterials. 2008 May;29(15):2348-58
pubmed: 18313138
Regen Med. 2014 Jan;9(1):89-100
pubmed: 24351009
J Appl Physiol (1985). 2013 Sep;115(6):937-48
pubmed: 23681911
Handchir Mikrochir Plast Chir. 2018 Apr;50(2):93-100
pubmed: 29378379
J Surg Res. 2015 Sep;198(1):50-6
pubmed: 26026854
Biochem Biophys Res Commun. 2019 Aug 20;516(2):558-564
pubmed: 31235253
Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):E3713-22
pubmed: 24019467
Cells. 2020 Jul 24;9(8):
pubmed: 32722232
AAPS J. 2017 Mar;19(2):431-437
pubmed: 27924614
Cell Prolif. 2019 Jul;52(4):e12631
pubmed: 31038259
Cell Metab. 2015 Jul 7;22(1):164-74
pubmed: 26001423
BMC Biotechnol. 2018 Nov 26;18(1):75
pubmed: 30477471
Lasers Med Sci. 2015 Nov;30(8):2209-13
pubmed: 25616713