Functional α7 nicotinic receptors in human airway smooth muscle increase intracellular calcium concentration and contractility in asthmatics.
acetylcholine receptor
asthma
calcium
contraction
nicotine
Journal
American journal of physiology. Lung cellular and molecular physiology
ISSN: 1522-1504
Titre abrégé: Am J Physiol Lung Cell Mol Physiol
Pays: United States
ID NLM: 100901229
Informations de publication
Date de publication:
01 07 2023
01 07 2023
Historique:
pmc-release:
01
07
2024
medline:
26
6
2023
pubmed:
16
5
2023
entrez:
16
5
2023
Statut:
ppublish
Résumé
Although nicotinic acetylcholine receptors (nAChRs) are commonly associated with neurons in the brain and periphery, recent data indicate that they are also expressed in non-neuronal tissues. We recently found the alpha7 (α7nAChR) subunit is highly expressed in human airway smooth muscle (hASM) with substantial increase in asthmatics, but their functionality remains unknown. We investigated the location and functional role of α7nAChRs in hASM cells from normal versus mild-moderate asthmatic patients. Immunostaining and protein analyses showed α7nAChR in the plasma membrane including in asthmatics. In asthmatic hASM, patch-clamp recordings revealed significantly higher functional homomeric α7nAChR channels. Real-time fluorescence imaging showed nicotine, via α7nAChR, increases intracellular Ca
Identifiants
pubmed: 37192375
doi: 10.1152/ajplung.00260.2022
pmc: PMC10292984
doi:
Substances chimiques
alpha7 Nicotinic Acetylcholine Receptor
0
Nicotine
6M3C89ZY6R
Calcium
SY7Q814VUP
Receptors, Nicotinic
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
L17-L29Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL088029
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL142061
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS031744
Pays : United States
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