Determination of pilus-islands profile and antibiotic susceptibility of
Antibiotic resistance
Bacterial pili
Group B Streptococcus
Pregnant women
Streptococcus agalactiae
Urine
Journal
Iranian journal of microbiology
ISSN: 2008-3289
Titre abrégé: Iran J Microbiol
Pays: Iran
ID NLM: 101518404
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
medline:
17
5
2023
pubmed:
17
5
2023
entrez:
16
5
2023
Statut:
ppublish
Résumé
Group B Streptococcus (GBS) is one of the most important causes of neonatal diseases and postpartum fever. GBS infection can be transmitted from the infected mother to her baby during delivery. This bacterium is also involved in causing urinary tract infections and asymptomatic bacteriuria, pyelonephritis, cystitis and urethritis. In addition to capsule, Pilus is known as a virulence factor of GBS. The aim of this study was to evaluate the frequency of pilus islands and antibiotic resistance in GBS isolated from urine of pregnant women in Yazd, Iran. In this cross-sectional study, 33 GBS samples isolated from the urine of pregnant women were studied by the multiplex polymerase chain reaction (PCR) method for the presence of pilus islands PI-1, PI-2a and PI-2b. Antibiotic resistance phenotype of tetracycline, penicillin, gentamicin, erythromycin, levofloxacin and clindamycin was determined by disk diffusion method. Data were analyzed using SPSS, version 16. PI-1+PI-2a was the most frequent pilus island in the GBS isolates 28 (84.8%) and the frequency of PI-2b was 5 (15.2%). The frequency of PI-1+PI-2a was 50% in serotype III and 25%, 14.3%, 7.1% and 3.6% in serotypes Ia, II, Ib and V respectively (P=0.492). The sensitivity of all GBS isolates to penicillin was 93.9% and highest resistance to tetracycline (97%), clindamycin (24.2%) and erythromycin (21.2%). Most of the GBS urine isolates examined carried the PI-1+PI-2a gene, which increases bacterial potency in colonization and resistance to the immune system. Penicillin was best choice for prevention.
Sections du résumé
Background and Objectives
UNASSIGNED
Group B Streptococcus (GBS) is one of the most important causes of neonatal diseases and postpartum fever. GBS infection can be transmitted from the infected mother to her baby during delivery. This bacterium is also involved in causing urinary tract infections and asymptomatic bacteriuria, pyelonephritis, cystitis and urethritis. In addition to capsule, Pilus is known as a virulence factor of GBS. The aim of this study was to evaluate the frequency of pilus islands and antibiotic resistance in GBS isolated from urine of pregnant women in Yazd, Iran.
Materials and Methods
UNASSIGNED
In this cross-sectional study, 33 GBS samples isolated from the urine of pregnant women were studied by the multiplex polymerase chain reaction (PCR) method for the presence of pilus islands PI-1, PI-2a and PI-2b. Antibiotic resistance phenotype of tetracycline, penicillin, gentamicin, erythromycin, levofloxacin and clindamycin was determined by disk diffusion method. Data were analyzed using SPSS, version 16.
Results
UNASSIGNED
PI-1+PI-2a was the most frequent pilus island in the GBS isolates 28 (84.8%) and the frequency of PI-2b was 5 (15.2%). The frequency of PI-1+PI-2a was 50% in serotype III and 25%, 14.3%, 7.1% and 3.6% in serotypes Ia, II, Ib and V respectively (P=0.492). The sensitivity of all GBS isolates to penicillin was 93.9% and highest resistance to tetracycline (97%), clindamycin (24.2%) and erythromycin (21.2%).
Conclusion
UNASSIGNED
Most of the GBS urine isolates examined carried the PI-1+PI-2a gene, which increases bacterial potency in colonization and resistance to the immune system. Penicillin was best choice for prevention.
Identifiants
pubmed: 37193229
doi: 10.18502/ijm.v15i2.12473
pii: IJM-15-219
pmc: PMC10183068
doi:
Types de publication
Journal Article
Langues
eng
Pagination
219-224Informations de copyright
Copyright © 2023 The Authors. Published by Tehran University of Medical Sciences.
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