The brain's dark transcriptome: Sequencing RNA in distal compartments of neurons and glia.
Journal
Current opinion in neurobiology
ISSN: 1873-6882
Titre abrégé: Curr Opin Neurobiol
Pays: England
ID NLM: 9111376
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
09
11
2022
revised:
22
03
2023
accepted:
02
04
2023
medline:
8
8
2023
pubmed:
18
5
2023
entrez:
17
5
2023
Statut:
ppublish
Résumé
Transcriptomic approaches are powerful strategies to map the molecular diversity of cells in the brain. Single-cell genomic atlases have now been compiled for entire mammalian brains. However, complementary techniques are only just beginning to map the subcellular transcriptomes from distal cellular compartments. We review single-cell datasets alongside subtranscriptome data from the mammalian brain to explore the development of cellular and subcellular diversity. We discuss how single-cell RNA-seq misses transcripts localized away from cell bodies, which form the 'dark transcriptome' of the brain: a collection of subtranscriptomes in dendrites, axons, growth cones, synapses, and endfeet with important roles in brain development and function. Recent advances in subcellular transcriptome sequencing are beginning to reveal these elusive pools of RNA. We outline the success stories to date in uncovering the constituent subtranscriptomes of neurons and glia, as well as present the emerging toolkit that is accelerating the pace of subtranscriptome discovery.
Identifiants
pubmed: 37196598
pii: S0959-4388(23)00050-8
doi: 10.1016/j.conb.2023.102725
pmc: PMC10524153
mid: NIHMS1891335
pii:
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Review
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
102725Subventions
Organisme : NIDA NIH HHS
ID : U01 DA051947
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA056440
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA054905
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH129343
Pays : United States
Organisme : NIMH NIH HHS
ID : R21 MH128462
Pays : United States
Organisme : NIMH NIH HHS
ID : DP2 MH122398
Pays : United States
Organisme : NIMH NIH HHS
ID : R24 MH114788
Pays : United States
Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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