Bacteriospermia and its antimicrobial resistance in relation to boar sperm quality during short-term storage with or without antibiotics in a tropical environment.
Acrosome integrity
Antibiotics
Boar
Chilled semen
Seminal bacteria
Journal
Porcine health management
ISSN: 2055-5660
Titre abrégé: Porcine Health Manag
Pays: England
ID NLM: 101684126
Informations de publication
Date de publication:
17 May 2023
17 May 2023
Historique:
received:
05
04
2023
accepted:
08
05
2023
medline:
18
5
2023
pubmed:
18
5
2023
entrez:
17
5
2023
Statut:
epublish
Résumé
In tropical environments, boar semen is prepared either from a boar on the same farm as the sow herd or collected in semen collection centers and then transported to other farms. Thus, the semen doses can be used for artificial insemination either immediately or preserved for 2-3 days. The present study investigated the bacteriospermia and its antimicrobial resistance in relation to boar sperm quality during short-term storage in semen extender with or without antibiotics in Thailand. M&M: In total, 20 Duroc ejaculates were collected. Each ejaculate was diluted in Beltsville Thawing Solution extender either with 0.25 g of gentamicin per liter (ANTIBIOTIC) or without gentamicin (NO-ANITIBIOTIC) to create semen doses containing 3,000 × 10 Sperm viability was decreased by 6.4% for every 1.0 log Our findings contribute new insights toward reducing antibiotics as well as rational antibiotic use in the boar AI industry. The growth of bacteria was significantly greater only after 2 days of preservation in the semen without antibiotic. For semen doses diluted from highly viable ejaculates, it is possible to store for 2 days without any antibiotic supplementation. Moreover, bacterial counts increased at the end of storage in the presence of gentamycin, suggesting the loss of bacteriostatic properties of gentamicin to the growth of bacteria during storage.
Sections du résumé
BACKGROUND
BACKGROUND
In tropical environments, boar semen is prepared either from a boar on the same farm as the sow herd or collected in semen collection centers and then transported to other farms. Thus, the semen doses can be used for artificial insemination either immediately or preserved for 2-3 days. The present study investigated the bacteriospermia and its antimicrobial resistance in relation to boar sperm quality during short-term storage in semen extender with or without antibiotics in Thailand. M&M: In total, 20 Duroc ejaculates were collected. Each ejaculate was diluted in Beltsville Thawing Solution extender either with 0.25 g of gentamicin per liter (ANTIBIOTIC) or without gentamicin (NO-ANITIBIOTIC) to create semen doses containing 3,000 × 10
RESULTS
RESULTS
Sperm viability was decreased by 6.4% for every 1.0 log
CONCLUSION
CONCLUSIONS
Our findings contribute new insights toward reducing antibiotics as well as rational antibiotic use in the boar AI industry. The growth of bacteria was significantly greater only after 2 days of preservation in the semen without antibiotic. For semen doses diluted from highly viable ejaculates, it is possible to store for 2 days without any antibiotic supplementation. Moreover, bacterial counts increased at the end of storage in the presence of gentamycin, suggesting the loss of bacteriostatic properties of gentamicin to the growth of bacteria during storage.
Identifiants
pubmed: 37198655
doi: 10.1186/s40813-023-00320-2
pii: 10.1186/s40813-023-00320-2
pmc: PMC10193611
doi:
Types de publication
Journal Article
Langues
eng
Pagination
21Subventions
Organisme : Thailand Science Research and Innovation Fund, Chulalongkorn University
ID : FOOD66310009
Organisme : The National Research Council of Thailand (NRCT)
ID : N42A660892
Informations de copyright
© 2023. The Author(s).
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