Transcobalamin receptor gene polymorphisms and mutation in an elderly population.

CD320 Cobalamin Gene Polymorphisms Transcobalamin Transcobalamin receptor Vitamin B12

Journal

Clinical nutrition ESPEN
ISSN: 2405-4577
Titre abrégé: Clin Nutr ESPEN
Pays: England
ID NLM: 101654592

Informations de publication

Date de publication:
06 2023
Historique:
received: 03 11 2022
revised: 05 04 2023
accepted: 27 04 2023
medline: 22 5 2023
pubmed: 19 5 2023
entrez: 18 5 2023
Statut: ppublish

Résumé

Cellular uptake of the essential nutrient vitamin B12 (cobalamin) occurs via the transcobalamin receptor (TCblR/CD320), a ubiquitous membrane receptor. Polymorphisms in the receptor exist, though the effect of such variants across patient populations is unknown. We determined CD320 genotype in 377 randomly selected elderly individuals. Three polymorphisms and a codon deletion were identified in the exon 2 region. Haplotype variants had significantly higher holotranscobalamin (holo-TC) values and a higher holo-TC/total cobalamin ratio. TCblR haplotype explained 46% of the variability in holo-TC values. This has significant implications for the clinical utility of the 'combined indicator' of B12 status since it is based on a standard rate of intracellular flux via the TC-Cbl receptor. Modification of the model may be required to account for CD320 haplotype.

Sections du résumé

BACKGROUND & AIMS
Cellular uptake of the essential nutrient vitamin B12 (cobalamin) occurs via the transcobalamin receptor (TCblR/CD320), a ubiquitous membrane receptor. Polymorphisms in the receptor exist, though the effect of such variants across patient populations is unknown.
METHODS
We determined CD320 genotype in 377 randomly selected elderly individuals.
RESULTS
Three polymorphisms and a codon deletion were identified in the exon 2 region. Haplotype variants had significantly higher holotranscobalamin (holo-TC) values and a higher holo-TC/total cobalamin ratio. TCblR haplotype explained 46% of the variability in holo-TC values.
CONCLUSIONS
This has significant implications for the clinical utility of the 'combined indicator' of B12 status since it is based on a standard rate of intracellular flux via the TC-Cbl receptor. Modification of the model may be required to account for CD320 haplotype.

Identifiants

pubmed: 37202078
pii: S2405-4577(23)00116-X
doi: 10.1016/j.clnesp.2023.04.023
pii:
doi:

Substances chimiques

Receptors, Cell Surface 0
transcobalamin receptor 0
Vitamin B 12 P6YC3EG204
CD320 protein, human 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

425-427

Subventions

Organisme : Medical Research Council
Pays : United Kingdom

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest No author had any conflict of interest to declare.

Auteurs

Andrew McCaddon (A)

Faculty of Social and Life Sciences, Wrexham Glyndwr University, Wrexham, UK. Electronic address: mccaddon@sky.com.

Daniel F Carr (DF)

Department of Pharmacology and Therapeutics, University of Liverpool, UK.

Hudson Peter (H)

COBALZ Limited, 3 Grove Road, Wrexham, UK.

Stuart J Moat (SJ)

School of Medicine and Department of Medical Biochemistry, Cardiff University, UK.

Edward V Quadros (EV)

Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY, USA.

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Classifications MeSH