Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environment.
A549
Air–liquid interface
Cell culture
Cell lines
Chemically defined media
Fetal bovine serum
Serum-free media
Xeno-free media
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
22
5
2023
pubmed:
19
5
2023
entrez:
18
5
2023
Statut:
ppublish
Résumé
The human lung adenocarcinoma cell line A549 is commonly used in cancer research as a model of malignant alveolar type II epithelial cells. A549 cells are frequently cultured in Ham's F12K (Kaighn's) or Dulbecco's Modified Eagle's Medium (DMEM), supplemented with glutamine and 10% fetal bovine serum (FBS). However, the use of FBS presents significant scientific concerns, such as the presence of undefined components and batch-to-batch variation leading to possible reproducibility issues in experiments and readouts. This chapter describes how to transition A549 cells to FBS-free medium and gives some insights on the further characterizations and functionality assays that would be necessary to perform for the validation of the cultured cells.
Identifiants
pubmed: 37202617
doi: 10.1007/978-1-0716-3056-3_8
doi:
Substances chimiques
Culture Media
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
165-172Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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