Translocation of bacterial LPS is associated with self-reported cognitive abilities in men living with HIV receiving antiretroviral therapy.


Journal

AIDS research and therapy
ISSN: 1742-6405
Titre abrégé: AIDS Res Ther
Pays: England
ID NLM: 101237921

Informations de publication

Date de publication:
18 05 2023
Historique:
received: 07 02 2023
accepted: 11 05 2023
medline: 22 5 2023
pubmed: 19 5 2023
entrez: 18 5 2023
Statut: epublish

Résumé

Gut damage allows translocation of bacterial lipopolysaccharide (LPS) and fungal β-D-glucan (BDG) into the blood. This microbial translocation contributes to systemic inflammation and risk of non-AIDS comorbidities in people living with HIV, including those receiving antiretroviral therapy (ART). We assessed whether markers of gut damage and microbial translocation were associated with cognition in ART-treated PLWH. Eighty ART-treated men living with HIV from the Positive Brain Health Now Canadian cohort were included. Brief cognitive ability measure (B-CAM) and 20-item patient deficit questionnaire (PDQ) were administered to all participants. Three groups were selected based on their B-CAM levels. We excluded participants who received proton pump inhibitors or antiacids in the past 3 months. Cannabis users were also excluded. Plasma levels of intestinal fatty acid binding protein (I-FABP), regenerating islet-derived protein 3 α (REG3α), and lipopolysaccharides (LPS = were quantified by ELISA, while 1-3-β-D-glucan BDG) levels were assessed using the Fungitell assay. Univariable, multivariable, and splines analyses were performed. Plasma levels of I-FABP, REG3α, LPS and BDG were not different between groups of low, intermediate and high B-CAM levels. However, LPS and REG3α levels were higher in participants with PDQ higher than the median. Multivariable analyses showed that LPS association with PDQ, but not B-CAM, was independent of age and level of education. I-FABP, REG3α, and BDG levels were not associated with B-CAM nor PDQ levels in multivariable analyses. In this well characterized cohort of ART-treated men living with HIV, bacterial but not fungal translocation was associated with presence of cognitive difficulties. These results need replication in larger samples.

Sections du résumé

BACKGROUND
Gut damage allows translocation of bacterial lipopolysaccharide (LPS) and fungal β-D-glucan (BDG) into the blood. This microbial translocation contributes to systemic inflammation and risk of non-AIDS comorbidities in people living with HIV, including those receiving antiretroviral therapy (ART). We assessed whether markers of gut damage and microbial translocation were associated with cognition in ART-treated PLWH.
METHODS
Eighty ART-treated men living with HIV from the Positive Brain Health Now Canadian cohort were included. Brief cognitive ability measure (B-CAM) and 20-item patient deficit questionnaire (PDQ) were administered to all participants. Three groups were selected based on their B-CAM levels. We excluded participants who received proton pump inhibitors or antiacids in the past 3 months. Cannabis users were also excluded. Plasma levels of intestinal fatty acid binding protein (I-FABP), regenerating islet-derived protein 3 α (REG3α), and lipopolysaccharides (LPS = were quantified by ELISA, while 1-3-β-D-glucan BDG) levels were assessed using the Fungitell assay. Univariable, multivariable, and splines analyses were performed.
RESULTS
Plasma levels of I-FABP, REG3α, LPS and BDG were not different between groups of low, intermediate and high B-CAM levels. However, LPS and REG3α levels were higher in participants with PDQ higher than the median. Multivariable analyses showed that LPS association with PDQ, but not B-CAM, was independent of age and level of education. I-FABP, REG3α, and BDG levels were not associated with B-CAM nor PDQ levels in multivariable analyses.
CONCLUSION
In this well characterized cohort of ART-treated men living with HIV, bacterial but not fungal translocation was associated with presence of cognitive difficulties. These results need replication in larger samples.

Identifiants

pubmed: 37202809
doi: 10.1186/s12981-023-00525-z
pii: 10.1186/s12981-023-00525-z
pmc: PMC10193796
doi:

Substances chimiques

Lipopolysaccharides 0
Biomarkers 0
Glucans 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

30

Subventions

Organisme : CIHR
ID : MOP 103230
Pays : Canada
Organisme : CIHR
ID : PJT 166049
Pays : Canada
Organisme : CIHR
ID : TCO-125272
Pays : Canada

Informations de copyright

© 2023. The Author(s).

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Auteurs

Stéphane Isnard (S)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada.
CIHR Canadian HIV Trials Network, Vancouver, BC, Canada.

Léna Royston (L)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada.
Division of Infectious Diseases, Geneva University Hospitals, Geneva, Switzerland.

Susan C Scott (SC)

Division of Clinical Epidemiology, Center for Outcomes Research and Evaluation, McGill University Health Centre (MUHC), Montreal, Canada.

Tsoarello Mabanga (T)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada.

John Lin (J)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada.

Brandon Fombuena (B)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada.

Simeng Bu (S)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada.

Carolina A Berini (CA)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada.

Mark S Goldberg (MS)

Department of Medicine, McGill University, Montreal, Canada.

Malcolm Finkelman (M)

Associates of Cape Cod Inc, Falmouth, MA, USA.

Marie-Josée Brouillette (MJ)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada.
Department of Psychiatry, McGill University, Montreal, Canada.

Lesley K Fellows (LK)

Department of Neurology & Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada.

Nancy E Mayo (NE)

Division of Clinical Epidemiology, Center for Outcomes Research and Evaluation, McGill University Health Centre (MUHC), Montreal, Canada.
School of Physical and Occupational Therapy, McGill University, Montreal, Canada.
Department of Medicine, Division of Geriatrics, McGill University, Montreal, Canada.

Jean-Pierre Routy (JP)

Infectious Disease and Immunity in Global Health Program, Research Institute of McGill University Health Centre, 1001 Boulevard Décarie, Montreal, QC, H4A 3J1, Canada. jean-pierre.routy@mcgill.ca.
Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada. jean-pierre.routy@mcgill.ca.
Division of Hematology, McGill University Health Centre, Montreal, QC, Canada. jean-pierre.routy@mcgill.ca.

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