Multi-walled carbon nanotubes elicit concordant changes in DNA methylation and gene expression following long-term pulmonary exposure in mice.
Carbon nanotubes
DNA methylation
Epigenetics
Inflammation
Transcriptomics
Journal
Carbon
ISSN: 0008-6223
Titre abrégé: Carbon N Y
Pays: United States
ID NLM: 101204394
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
medline:
1
6
2021
pubmed:
1
6
2021
entrez:
19
5
2023
Statut:
ppublish
Résumé
Pulmonary exposure to multi-walled carbon nanotubes (MWCNTs) causes inflammation and fibrosis. Our previous work has shown that industrially produced MWCNTs trigger specific changes in gene expression in the lungs of exposed animals. To elucidate whether epigenetic effects play a role for these gene expression changes, we performed whole genome bisulphite sequencing to assess DNA methylation patterns in the lungs 56 days after exposure to MWCNTs. Lung tissues were also evaluated with respect to histopathological changes and cytokine profiling of bronchoalveolar lavage (BAL) fluid was conducted using a multi-plex array. Integrated analysis of transcriptomics data and DNA methylation data revealed concordant changes in gene expression. Functional analysis showed that the muscle contraction, immune system/inflammation, and extracellular matrix pathways were the most affected pathways. Taken together, the present study revealed that MWCNTs exert epigenetic effects in the lungs of exposed animals, potentially driving the subsequent gene expression changes.
Identifiants
pubmed: 37206955
doi: 10.1016/j.carbon.2021.03.045
pmc: PMC10193301
mid: NIHMS1866907
doi:
Types de publication
Journal Article
Langues
eng
Pagination
563-572Subventions
Organisme : Intramural CDC HHS
ID : CC999999
Pays : United States
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no competing financial interests or other personal interests.
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