High-content CRISPR screening.
Journal
Nature reviews. Methods primers
ISSN: 2662-8449
Titre abrégé: Nat Rev Methods Primers
Pays: England
ID NLM: 101778010
Informations de publication
Date de publication:
2022
2022
Historique:
medline:
1
1
2022
pubmed:
1
1
2022
entrez:
22
5
2023
Statut:
ppublish
Résumé
CRISPR screens are a powerful source of biological discovery, enabling the unbiased interrogation of gene function in a wide range of applications and species. In pooled CRISPR screens, various genetically encoded perturbations are introduced into pools of cells. The targeted cells proliferate under a biological challenge such as cell competition, drug treatment or viral infection. Subsequently, the perturbation-induced effects are evaluated by sequencing-based counting of the guide RNAs that specify each perturbation. The typical results of such screens are ranked lists of genes that confer sensitivity or resistance to the biological challenge of interest. Contributing to the broad utility of CRISPR screens, adaptations of the core CRISPR technology make it possible to activate, silence or otherwise manipulate the target genes. Moreover, high-content read-outs such as single-cell RNA sequencing and spatial imaging help characterize screened cells with unprecedented detail. Dedicated software tools facilitate bioinformatic analysis and enhance reproducibility. CRISPR screening has unravelled various molecular mechanisms in basic biology, medical genetics, cancer research, immunology, infectious diseases, microbiology and other fields. This Primer describes the basic and advanced concepts of CRISPR screening and its application as a flexible and reliable method for biological discovery, biomedical research and drug development - with a special emphasis on high-content methods that make it possible to obtain detailed biological insights directly as part of the screen.
Identifiants
pubmed: 37214176
doi: 10.1038/s43586-022-00098-7
pmc: PMC10200264
mid: NIHMS1898606
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIDDK NIH HHS
ID : U01 DK127405
Pays : United States
Déclaration de conflit d'intérêts
C.B. is a co-founder and scientific advisor of Aelian Biotechnology and Neurolentech. S.C. is a co-founder of EvolveImmune Therapeutics and Cellinfinity Bio. M.G. has performed consultancy for Sanofi, receives research funding from AstraZeneca and GlaxoSmithKline, and is a co-founder of Mosaic Therapeutics. J.M. is a shareholder of Northern Biologics and Pionyr Immunotherapeutics, and a scientific advisor and shareholder of Century Therapeutics and Aelian Biotechnology. L.S.Q. is a co-founder and scientific advisor of Epicrispr Biotechnologies and Refuge Biotechnologies. J.S. is a scientific advisor of Maze Therapeutics, Camp4 Therapeutics, Cajal Biosciences, Adaptive Biotechnologies and Guardant Health, and a co-founder of Scale Bio and Phase Genomics. J.S.W. consults for and holds equity in KSQ Therapeutics, Maze Therapeutics and Tenaya Therapeutics, is a venture partner at 5AM Ventures and is a member of the Amgen Scientific Advisory Board. X.Z. is a co-founder and consultant of Vizgen. The other authors declare no competing interests.
Références
Nat Cell Biol. 2020 Mar;22(3):321-331
pubmed: 32123335
Drug Discov Today. 2020 Aug;25(8):1348-1361
pubmed: 32561299
Nat Biotechnol. 2021 May;39(5):619-629
pubmed: 33558698
Nat Commun. 2020 Jun 12;11(1):2973
pubmed: 32532987
Nat Rev Genet. 2002 Mar;3(3):176-88
pubmed: 11972155
Nat Genet. 2018 Dec;50(12):1716-1727
pubmed: 30397336
Cell Syst. 2020 Nov 18;11(5):523-535.e9
pubmed: 33080209
Nat Methods. 2018 Aug;15(8):611-616
pubmed: 30013045
Nat Commun. 2017 Jul 13;8:15993
pubmed: 28703221
Science. 2018 Feb 16;359(6377):770-775
pubmed: 29301958
Nat Commun. 2021 Mar 12;12(1):1661
pubmed: 33712601
Nat Biotechnol. 2012 Mar 07;30(3):224-6
pubmed: 22398613
Nat Methods. 2017 Jun;14(6):577-580
pubmed: 28481362
Elife. 2017 Dec 05;6:
pubmed: 29206104
Nat Genet. 2019 Jul;51(7):1149-1159
pubmed: 31253978
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
Cell. 2021 Apr 29;184(9):2394-2411.e16
pubmed: 33743211
Cell Rep. 2020 Dec 29;33(13):108541
pubmed: 33378675
Nat Commun. 2018 Jun 5;9(1):2184
pubmed: 29872041
Sci Adv. 2020 Sep 18;6(38):
pubmed: 32948588
Nat Biotechnol. 2021 Nov;39(11):1414-1425
pubmed: 34183861
Nat Genet. 2018 Mar;50(3):460-471
pubmed: 29459677
Genome Biol. 2021 Jan 21;22(1):40
pubmed: 33478580
Nature. 2021 Sep;597(7877):549-554
pubmed: 34497417
Genome Biol. 2020 Mar 2;21(1):53
pubmed: 32122365
Nat Methods. 2015 Apr;12(4):326-8
pubmed: 25730490
Nat Methods. 2020 Jul;17(7):708-716
pubmed: 32514112
Nat Commun. 2018 Feb 9;9(1):597
pubmed: 29426832
Genome Biol. 2021 Dec 20;22(1):343
pubmed: 34930405
Nat Biomed Eng. 2021 Feb;5(2):190-194
pubmed: 32989284
BMC Bioinformatics. 2017 Jul 21;18(1):347
pubmed: 28732459
Nat Microbiol. 2021 Mar;6(3):301-312
pubmed: 33462433
Nat Commun. 2021 May 20;12(1):2969
pubmed: 34016988
Nat Methods. 2017 Mar;14(3):297-301
pubmed: 28099430
Science. 2015 Apr 24;348(6233):aaa6090
pubmed: 25858977
Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519
pubmed: 27729526
Cell. 2015 Oct 22;163(3):759-71
pubmed: 26422227
Nat Commun. 2019 Sep 3;10(1):3963
pubmed: 31481656
Mol Cell. 2020 May 21;78(4):779-784.e5
pubmed: 32362314
Nature. 2015 Jan 29;517(7536):583-8
pubmed: 25494202
Nat Commun. 2018 Jun 26;9(1):2475
pubmed: 29946130
Sci Adv. 2017 Aug 30;3(8):eaao4774
pubmed: 28875174
Nat Biotechnol. 2022 Feb;40(2):218-226
pubmed: 34650269
Science. 2014 Jan 3;343(6166):84-87
pubmed: 24336571
Trends Biotechnol. 2019 Oct;37(10):1121-1142
pubmed: 30995964
Neuron. 2019 Dec 4;104(5):885-898.e8
pubmed: 31587919
Nat Biotechnol. 2015 May;33(5):510-7
pubmed: 25849900
BMC Genomics. 2018 Aug 13;19(1):604
pubmed: 30103702
Cell Stem Cell. 2020 May 7;26(5):782-792.e7
pubmed: 32348727
G3 (Bethesda). 2017 Aug 7;7(8):2719-2727
pubmed: 28655737
Elife. 2020 Nov 06;9:
pubmed: 33155979
FEMS Microbiol Rev. 2021 May 5;45(3):
pubmed: 33145589
Nat Biotechnol. 2020 Aug;38(8):954-961
pubmed: 32231336
Cell. 2021 Jan 7;184(1):106-119.e14
pubmed: 33333024
Nat Methods. 2018 Dec;15(12):992-993
pubmed: 30504875
Oncogene. 2020 Jan;39(1):30-35
pubmed: 31467430
Nat Rev Genet. 2015 May;16(5):299-311
pubmed: 25854182
Genome Biol. 2020 Mar 9;21(1):62
pubmed: 32151271
Nat Biotechnol. 2016 Feb;34(2):184-191
pubmed: 26780180
Nat Commun. 2017 May 12;8:15315
pubmed: 28497787
Science. 2019 Aug 23;365(6455):786-793
pubmed: 31395745
Nat Methods. 2020 Jan;17(1):86-92
pubmed: 31740817
Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E180-E189
pubmed: 29269392
Cell. 2015 Jan 15;160(1-2):339-50
pubmed: 25533786
Cell. 2021 Mar 4;184(5):1262-1280.e22
pubmed: 33636129
Nat Commun. 2017 May 05;8:15178
pubmed: 28474669
Genome Biol. 2020 Oct 15;21(1):262
pubmed: 33059726
Nature. 2020 Apr;580(7801):136-141
pubmed: 32238925
Nat Commun. 2021 Feb 11;12(1):961
pubmed: 33574281
Nat Microbiol. 2018 Jan;3(1):73-82
pubmed: 29062088
Cell. 2021 Oct 28;184(22):5635-5652.e29
pubmed: 34653350
Nat Methods. 2016 Dec;13(12):1036-1042
pubmed: 27798611
Cell. 2016 Dec 15;167(7):1853-1866.e17
pubmed: 27984732
Nat Genet. 2021 Mar;53(3):322-331
pubmed: 33649593
Nat Rev Drug Discov. 2020 Jan;19(1):23-38
pubmed: 31712683
Cell. 2016 Dec 15;167(7):1883-1896.e15
pubmed: 27984734
Science. 2018 Apr 13;360(6385):176-182
pubmed: 29545511
Nat Commun. 2017 Sep 15;8(1):560
pubmed: 28916764
Nature. 2005 Apr 14;434(7035):917-21
pubmed: 15829967
Cell. 2018 Dec 13;175(7):1958-1971.e15
pubmed: 30449619
Cell Rep. 2019 Apr 9;27(2):599-615.e12
pubmed: 30970261
Nat Biotechnol. 2022 Jun;40(6):885-895
pubmed: 35190686
Nat Commun. 2020 Apr 3;11(1):1666
pubmed: 32245970
Nat Methods. 2021 Aug;18(8):965-974
pubmed: 34341582
Genome Biol. 2018 Feb 6;19(1):15
pubmed: 29409532
Nat Rev Genet. 2001 Dec;2(12):956-66
pubmed: 11733748
Science. 2015 Nov 27;350(6264):1096-101
pubmed: 26472758
PLoS Genet. 2019 Oct 25;15(10):e1008466
pubmed: 31652272
Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18424-18430
pubmed: 32690674
Bioinformatics. 2016 Feb 15;32(4):587-9
pubmed: 26508757
Nat Commun. 2018 Aug 29;9(1):3509
pubmed: 30158531
Nature. 2014 Dec 11;516(7530):263-6
pubmed: 25274302
Nature. 2019 Dec;576(7787):471-476
pubmed: 31827283
Cell Rep. 2016 Oct 18;17(4):1193-1205
pubmed: 27760321
Nature. 2017 Aug 31;548(7669):537-542
pubmed: 28783722
Cell. 2019 Aug 22;178(5):1189-1204.e23
pubmed: 31442407
Mol Syst Biol. 2017 Oct 9;13(10):945
pubmed: 28993443
Cell. 2015 Jul 30;162(3):540-51
pubmed: 26232224
Cell Host Microbe. 2021 Jan 13;29(1):107-120.e6
pubmed: 33120116
Genome Res. 2019 Apr;29(4):668-681
pubmed: 30782640
Nat Commun. 2019 Dec 20;10(1):5817
pubmed: 31862961
PLoS Pathog. 2019 Jun 26;15(6):e1007828
pubmed: 31242261
Bioinformatics. 2012 Oct 1;28(19):2520-2
pubmed: 22908215
Nat Neurosci. 2021 Jul;24(7):1020-1034
pubmed: 34031600
Mol Cell. 2017 Apr 20;66(2):285-299.e5
pubmed: 28416141
Science. 2018 Jul 20;361(6399):285-290
pubmed: 30026227
Nature. 2015 Aug 13;524(7564):230-3
pubmed: 26168398
Science. 2020 Mar 27;367(6485):1468-1473
pubmed: 32029688
Cancer Discov. 2016 Aug;6(8):914-29
pubmed: 27260156
Nat Methods. 2018 Nov;15(11):924-927
pubmed: 30377362
PLoS Pathog. 2020 Mar 9;16(3):e1008344
pubmed: 32150575
Nat Methods. 2019 May;16(5):405-408
pubmed: 30962622
Nature. 2020 Jul;583(7815):296-302
pubmed: 32612232
Nat Immunol. 2020 Nov;21(11):1456-1466
pubmed: 32989329
Nature. 2019 Apr;568(7753):511-516
pubmed: 30971826
Elife. 2019 Mar 25;8:
pubmed: 30910006
Cell. 2018 Aug 9;174(4):953-967.e22
pubmed: 30033366
Annu Rev Biochem. 2016 Jun 2;85:227-64
pubmed: 27145843
Nat Rev Genet. 2018 Feb;19(2):67-80
pubmed: 29199283
Nat Biotechnol. 2020 May;38(5):638-648
pubmed: 32249828
Nat Rev Microbiol. 2018 Jun;16(6):333-339
pubmed: 29599458
BMC Genomics. 2021 Nov 17;22(1):828
pubmed: 34789150
Genome Biol. 2018 Oct 8;19(1):159
pubmed: 30296940
PLoS Biol. 2020 Oct 13;18(10):e3000877
pubmed: 33048924
Cancer Discov. 2018 Sep;8(9):1069-1086
pubmed: 30115704
Nat Methods. 2015 May;12(5):401-403
pubmed: 25775043
Nature. 2005 Apr 14;434(7035):913-7
pubmed: 15829966
Nature. 2019 Apr;568(7753):551-556
pubmed: 30971823
Nat Biotechnol. 2022 Feb;40(2):227-234
pubmed: 34650270
Genome Biol. 2019 Jan 24;20(1):20
pubmed: 30678704
Nat Biotechnol. 2021 Oct;39(10):1270-1277
pubmed: 33927415
Cell. 2020 Jul 23;182(2):463-480.e30
pubmed: 32533916
Bioinformatics. 2021 Nov 18;37(22):4266-4268
pubmed: 33999185
Mol Syst Biol. 2017 May 10;13(5):931
pubmed: 28490437
Science. 2014 Mar 21;343(6177):1360-3
pubmed: 24578530
Nat Methods. 2016 Dec;13(12):1043-1049
pubmed: 27776111
Nat Genet. 2019 Jun;51(6):999-1010
pubmed: 31110351
Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4051-E4060
pubmed: 29632189
Genome Biol. 2015 Dec 16;16:281
pubmed: 26673418
Curr Opin Microbiol. 2021 Feb;59:102-109
pubmed: 33285498
Cell. 2021 Jan 7;184(1):76-91.e13
pubmed: 33147444
Nat Methods. 2020 Nov;17(11):1093-1096
pubmed: 33020655
Cell. 2006 Mar 24;124(6):1283-98
pubmed: 16564017
Trends Biotechnol. 2016 Aug;34(8):605-608
pubmed: 27212022
Cell. 2017 Feb 23;168(5):890-903.e15
pubmed: 28162770
Nat Commun. 2018 Dec 21;9(1):5416
pubmed: 30575746
Annu Rev Pathol. 2017 Jan 24;12:187-215
pubmed: 27959627
Cell. 2021 Jan 7;184(1):120-132.e14
pubmed: 33382968
Science. 1996 Apr 12;272(5259):263-7
pubmed: 8602510
Sci Adv. 2021 Jul 2;7(27):
pubmed: 34215580
Cell. 2021 Feb 18;184(4):1064-1080.e20
pubmed: 33606977
Nat Rev Mol Cell Biol. 2014 Sep;15(9):591-600
pubmed: 25145850
Cell. 2021 Jan 7;184(1):133-148.e20
pubmed: 33338421
Nature. 2019 May;569(7756):433-437
pubmed: 30995674
Genome Biol. 2014;15(12):554
pubmed: 25476604
PLoS Biol. 2019 Apr 1;17(4):e2007044
pubmed: 30933966
Nat Protoc. 2019 Mar;14(3):756-780
pubmed: 30710114
Nat Methods. 2018 Apr;15(4):271-274
pubmed: 29457792
Nat Rev Genet. 2019 Sep;20(9):520-535
pubmed: 31235872
Nature. 2015 Nov 12;527(7577):192-7
pubmed: 26375006
Proc Natl Acad Sci U S A. 2019 Aug 20;116(34):17013-17022
pubmed: 31371515
Nucleic Acids Res. 2021 Jun 4;49(10):5684-5704
pubmed: 33956155
PLoS Biol. 2018 Jul 3;16(7):e2005970
pubmed: 29969450
J Mol Biol. 2019 Jan 4;431(1):34-47
pubmed: 29958882
Nature. 2017 Jul 27;547(7664):413-418
pubmed: 28723893
Nat Biotechnol. 2022 May;40(5):731-740
pubmed: 34887556
Cell. 2020 Mar 5;180(5):1002-1017.e31
pubmed: 32109417
Cell. 2018 Nov 15;175(5):1405-1417.e14
pubmed: 30318144
Annu Rev Immunol. 2019 Apr 26;37:521-546
pubmed: 30726153
Nat Commun. 2021 Oct 22;12(1):6150
pubmed: 34686672
Cell. 2021 Jan 7;184(1):92-105.e16
pubmed: 33147445
Genome Res. 2019 Jun;29(6):999-1008
pubmed: 31015259
Nature. 2019 Dec;576(7785):149-157
pubmed: 31634902
Nat Rev Genet. 2004 Mar;5(3):179-89
pubmed: 14970820
Nat Methods. 2013 Sep;10(9):857-60
pubmed: 23852452
Nat Biotechnol. 2021 Feb;39(2):198-206
pubmed: 32958957
Cell Syst. 2015 Dec 23;1(6):417-425
pubmed: 26771021
Nat Methods. 2019 Nov;16(11):1087-1093
pubmed: 31659326
Cell. 2019 Oct 17;179(3):787-799.e17
pubmed: 31626775
Nat Commun. 2021 Nov 4;12(1):6395
pubmed: 34737291
Nature. 2017 Nov 23;551(7681):464-471
pubmed: 29160308
Cell. 2014 Oct 23;159(3):635-46
pubmed: 25307933
Nat Methods. 2019 Jul;16(7):633-639
pubmed: 31235883
Cell. 2013 Feb 28;152(5):1173-83
pubmed: 23452860
Nat Genet. 2019 Sep;51(9):1389-1398
pubmed: 31477929
Genome Res. 2019 Mar;29(3):464-471
pubmed: 30674557
Nat Commun. 2020 Jul 13;11(1):3455
pubmed: 32661245
Nucleic Acids Res. 2017 Feb 28;45(4):1703-1713
pubmed: 27899645
Nat Biotechnol. 2022 Mar;40(3):391-401
pubmed: 34697476
Nat Biotechnol. 2023 Apr;41(4):500-512
pubmed: 36424489
Nat Commun. 2020 Oct 7;11(1):5041
pubmed: 33028827
Cell Syst. 2021 Jan 20;12(1):56-67.e6
pubmed: 33238135
Nat Commun. 2017 Dec 13;8(1):2095
pubmed: 29235474
Proc Natl Acad Sci U S A. 2021 Jan 26;118(4):
pubmed: 33483422
Cell. 2021 Mar 4;184(5):1245-1261.e21
pubmed: 33636132
Elife. 2016 Sep 23;5:
pubmed: 27661255
Cell. 2021 Apr 29;184(9):2503-2519.e17
pubmed: 33838111
Annu Rev Genomics Hum Genet. 2018 Aug 31;19:15-41
pubmed: 29727584
Science. 2017 Nov 24;358(6366):1019-1027
pubmed: 29070703
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Cell Stem Cell. 2018 Nov 1;23(5):758-771.e8
pubmed: 30318302
Nat Genet. 2021 Mar;53(3):332-341
pubmed: 33649592
ACS Synth Biol. 2020 May 15;9(5):1030-1040
pubmed: 32268068
Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916
pubmed: 28973434
Cell. 2019 Jan 10;176(1-2):361-376.e17
pubmed: 30580963
Cell. 2015 Jul 30;162(3):675-86
pubmed: 26189680
Nat Commun. 2019 May 20;10(1):2233
pubmed: 31110232
Nat Methods. 2019 Aug;16(8):715-721
pubmed: 31363220
Epigenetics Chromatin. 2017 May 8;10:24
pubmed: 28503202
Cancer Cell. 2019 Apr 15;35(4):545-557
pubmed: 30827888
Nat Rev Genet. 2019 May;20(5):257-272
pubmed: 30696980
Nucleic Acids Res. 2019 Jan 8;47(D1):D529-D541
pubmed: 30476227
Nat Methods. 2014 Aug;11(8):783-784
pubmed: 25075903
Science. 2018 Nov 9;362(6415):694-699
pubmed: 30409884
Cell. 2019 Feb 7;176(4):882-896.e18
pubmed: 30639098
Genome Biol. 2018 Aug 15;19(1):107
pubmed: 30107853
Nat Biotechnol. 2022 Mar;40(3):402-410
pubmed: 34608327
Nat Genet. 2021 Aug;53(8):1166-1176
pubmed: 34326544
Cell. 2013 Jul 18;154(2):442-51
pubmed: 23849981
Bioinformatics. 2015 Nov 15;31(22):3676-8
pubmed: 26209430
J Cell Biol. 2021 Feb 1;220(2):
pubmed: 33464298
Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13384-13393
pubmed: 31213543
Nat Methods. 2019 May;16(5):409-412
pubmed: 31011186
Cell. 2015 Dec 3;163(6):1515-26
pubmed: 26627737
Nat Biotechnol. 2018 Nov;36(10):977-982
pubmed: 30059493
Nat Microbiol. 2019 Feb;4(2):244-250
pubmed: 30617347
PLoS Genet. 2018 Nov 7;14(11):e1007749
pubmed: 30403660
Cell. 2018 Nov 1;175(4):1141-1155.e16
pubmed: 30343902
Nat Methods. 2020 Jun;17(6):629-635
pubmed: 32483332
Sci Immunol. 2018 May 18;3(23):
pubmed: 29776993
Nat Rev Genet. 2018 Dec;19(12):770-788
pubmed: 30323312
Cell. 2016 Dec 15;167(7):1867-1882.e21
pubmed: 27984733
Cell. 2019 Jan 10;176(1-2):377-390.e19
pubmed: 30612741
iScience. 2019 Mar 29;13:488-497
pubmed: 30898619
Nat Metab. 2020 Jun;2(6):499-513
pubmed: 32694731
Cell. 2021 Oct 28;184(22):5653-5669.e25
pubmed: 34672952
Nat Methods. 2019 Sep;16(9):887-893
pubmed: 31406383
Nat Methods. 2021 Jun;18(6):635-642
pubmed: 34059827
Nat Microbiol. 2019 Jul;4(7):1105-1113
pubmed: 30962569
Nat Genet. 2021 Aug;53(8):1196-1206
pubmed: 34253920
PLoS One. 2014 Aug 28;9(8):e105779
pubmed: 25166277
Nature. 2018 Oct;562(7726):217-222
pubmed: 30209399
Nat Methods. 2020 Jun;17(6):636-642
pubmed: 32393832
Cell. 2014 Oct 9;159(2):440-55
pubmed: 25263330
Mol Syst Biol. 2017 Oct 17;13(10):947
pubmed: 29042431
Nature. 2020 Oct;586(7827):120-126
pubmed: 32968282
Cancer Cell. 2020 Jun 8;37(6):818-833.e9
pubmed: 32516591
Nat Rev Genet. 2002 May;3(5):356-69
pubmed: 11988761
Nat Immunol. 2008 Oct;9(10):1091-4
pubmed: 18800157
Genetics. 2016 Jun;203(2):617-9
pubmed: 27270695
Nat Methods. 2017 Aug 31;14(9):849-863
pubmed: 28858338
Nat Biotechnol. 2019 Nov;37(11):1302-1313
pubmed: 31548728
Genome Med. 2019 Aug 22;11(1):52
pubmed: 31439014
Nat Commun. 2021 Feb 15;12(1):1034
pubmed: 33589617
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Genome Med. 2021 Jan 6;13(1):2
pubmed: 33407829
Science. 2014 Jan 3;343(6166):80-4
pubmed: 24336569
Nat Rev Genet. 2002 Oct;3(10):779-89
pubmed: 12360236
Nature. 2019 Feb;566(7745):496-502
pubmed: 30787437
Genome Biol. 2019 Feb 5;20(1):27
pubmed: 30722791
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Nat Genet. 2017 Dec;49(12):1779-1784
pubmed: 29083409
Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7
pubmed: 27141961
Cytometry A. 2019 Apr;95(4):366-380
pubmed: 30565841
Nat Biotechnol. 1997 Sep;15(9):871-5
pubmed: 9306402
Genome Biol. 2020 Jan 24;21(1):19
pubmed: 31980032
Nat Methods. 2017 Dec;14(12):1191-1197
pubmed: 29039415
Science. 2019 Jan 18;363(6424):
pubmed: 30545847
Cell. 2020 Apr 30;181(3):716-727.e11
pubmed: 32259488
Nat Biotechnol. 2020 Jun;38(6):722-727
pubmed: 32518401
PLoS Pathog. 2020 Jan 16;16(1):e1008201
pubmed: 31945142
Genome Biol. 2019 Jul 12;20(1):137
pubmed: 31300006
Genome Biol. 2017 Jun 14;18(1):108
pubmed: 28615073
Nat Genet. 2019 Dec;51(12):1664-1669
pubmed: 31784727
J Biol Chem. 2016 Jan 1;291(1):103-9
pubmed: 26553871
Bioinformatics. 2010 Apr 1;26(7):979-81
pubmed: 20338898
Nat Commun. 2019 Jul 17;10(1):3144
pubmed: 31316073
Science. 2020 Nov 27;370(6520):
pubmed: 33243861
Nat Immunol. 2019 Jul;20(7):770-774
pubmed: 31160798
Annu Rev Cell Dev Biol. 2016 Oct 6;32:1-46
pubmed: 27501451
Science. 2019 Jul 26;365(6451):382-386
pubmed: 31296651
Cell. 2014 Oct 23;159(3):647-61
pubmed: 25307932
Cell. 2017 Jul 27;170(3):564-576.e16
pubmed: 28753430
Genetics. 2021 Feb 9;217(2):
pubmed: 33724419
Mol Syst Biol. 2020 Jun;16(6):e9442
pubmed: 32500953
Nucleic Acids Res. 2017 Jan 4;45(D1):D362-D368
pubmed: 27924014
Nat Genet. 2021 Jun;53(6):869-880
pubmed: 33958780
Nature. 2021 Nov;599(7885):491-496
pubmed: 34711951
Nat Rev Genet. 2001 Sep;2(9):659-68
pubmed: 11533715
Nat Rev Genet. 2002 Feb;3(2):124-36
pubmed: 11836506
Curr Opin Genet Dev. 2018 Oct;52:70-76
pubmed: 29913329
Nat Biotechnol. 2017 Jan;35(1):48-55
pubmed: 27941803
Nat Biotechnol. 2015 Jun;33(6):661-7
pubmed: 25961408
Cell. 2021 Feb 18;184(4):1081-1097.e19
pubmed: 33606978
Nat Biotechnol. 2020 Jul;38(7):845-855
pubmed: 32601435
Nat Immunol. 2019 Oct;20(10):1335-1347
pubmed: 31527834
Cell. 2020 Jul 23;182(2):481-496.e21
pubmed: 32649862
Cell. 2021 Oct 14;184(21):5357-5374.e22
pubmed: 34582788
Cell. 2018 Apr 19;173(3):665-676.e14
pubmed: 29551272
Nat Rev Genet. 2008 Jul;9(7):554-66
pubmed: 18521077
Nat Genet. 2021 Apr;53(4):435-444
pubmed: 33686287
J Cell Biol. 2021 Feb 1;220(2):
pubmed: 33465779
Science. 2021 Apr 9;372(6538):
pubmed: 33833093
Mol Syst Biol. 2019 Jun 19;15(6):e8746
pubmed: 31217225
BMC Bioinformatics. 2016 Apr 16;17:164
pubmed: 27083490
J Clin Microbiol. 2019 Mar 28;57(4):
pubmed: 30429256
Nat Biotechnol. 2017 May;35(5):463-474
pubmed: 28319085
Nat Methods. 2017 Dec;14(12):1159-1162
pubmed: 29083401
Nucleic Acids Res. 2021 Jan 8;49(D1):D848-D854
pubmed: 33010154
Trends Parasitol. 2019 Jun;35(6):409-422
pubmed: 31006600
Mol Cell. 2021 Oct 21;81(20):4333-4345.e4
pubmed: 34480847
Nat Chem Biol. 2019 Sep;15(9):865-871
pubmed: 31383972
Cell. 2016 Sep 22;167(1):219-232.e14
pubmed: 27662090
Cell. 2015 Mar 12;160(6):1246-60
pubmed: 25748654
Cell Stem Cell. 2020 Mar 5;26(3):431-440.e8
pubmed: 32142663
Nat Protoc. 2021 Oct;16(10):4766-4798
pubmed: 34508259
Nat Chem Biol. 2015 Mar;11(3):198-200
pubmed: 25664691
Nat Biotechnol. 2018 Jul;36(6):540-546
pubmed: 29786095
Nat Methods. 2017 Jun;14(6):573-576
pubmed: 28319113
J Cell Biol. 2018 Nov 5;217(11):3817-3828
pubmed: 30093494
Science. 2020 Nov 20;370(6519):935-941
pubmed: 33122427
Nucleic Acids Res. 2014 Jan;42(Database issue):D975-9
pubmed: 24297256
Cell. 2016 Jun 2;165(6):1493-1506
pubmed: 27238023
Dev Cell. 2018 Jan 8;44(1):113-129.e8
pubmed: 29290584
Nat Commun. 2020 Apr 20;11(1):1871
pubmed: 32313011
Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851
pubmed: 31085639
Mol Syst Biol. 2014 Jul 01;10:733
pubmed: 24987113
Nat Biomed Eng. 2019 Oct;3(10):796-805
pubmed: 31548591
Nucleic Acids Res. 2015 Oct 15;43(18):8868-83
pubmed: 26350217
ACS Synth Biol. 2018 Dec 21;7(12):2775-2782
pubmed: 30424596
Cell. 2016 Sep 8;166(6):1423-1435.e12
pubmed: 27594426
Nat Biotechnol. 2018 Jul;36(6):505-508
pubmed: 29734295