Platelet-derived chemokines promote skeletal muscle regeneration by guiding neutrophil recruitment to injured muscles.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
22 05 2023
Historique:
received: 01 09 2022
accepted: 09 05 2023
medline: 24 5 2023
pubmed: 23 5 2023
entrez: 22 5 2023
Statut: epublish

Résumé

Skeletal muscle regeneration involves coordinated interactions between different cell types. Injection of platelet-rich plasma is circumstantially considered an aid to muscle repair but whether platelets promote regeneration beyond their role in hemostasis remains unexplored. Here, we find that signaling via platelet-released chemokines is an early event necessary for muscle repair in mice. Platelet depletion reduces the levels of the platelet-secreted neutrophil chemoattractants CXCL5 and CXCL7/PPBP. Consequently, early-phase neutrophil infiltration to injured muscles is impaired whereas later inflammation is exacerbated. Consistent with this model, neutrophil infiltration to injured muscles is compromised in male mice with Cxcl7-knockout platelets. Moreover, neo-angiogenesis and the re-establishment of myofiber size and muscle strength occurs optimally in control mice post-injury but not in Cxcl7ko mice and in neutrophil-depleted mice. Altogether, these findings indicate that platelet-secreted CXCL7 promotes regeneration by recruiting neutrophils to injured muscles, and that this signaling axis could be utilized therapeutically to boost muscle regeneration.

Identifiants

pubmed: 37217480
doi: 10.1038/s41467-023-38624-0
pii: 10.1038/s41467-023-38624-0
pmc: PMC10203137
doi:

Substances chimiques

Chemokines 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

2900

Subventions

Organisme : NIA NIH HHS
ID : R01 AG055532
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA245301
Pays : United States

Informations de copyright

© 2023. The Author(s).

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Auteurs

Flavia A Graca (FA)

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.

Anna Stephan (A)

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.

Benjamin A Minden-Birkenmaier (BA)

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
Department of Oncology, Division of Molecular Oncology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.

Abbas Shirinifard (A)

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.

Yong-Dong Wang (YD)

Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.

Fabio Demontis (F)

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA. Fabio.Demontis@stjude.org.

Myriam Labelle (M)

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA. Myriam.Labelle@stjude.org.
Department of Oncology, Division of Molecular Oncology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA. Myriam.Labelle@stjude.org.

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