Assessment of antimicrobial and anthelmintic activity of silver nanoparticles bio-synthesized from Viscum orientale leaf extract.


Journal

BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232

Informations de publication

Date de publication:
22 May 2023
Historique:
received: 29 07 2022
accepted: 27 04 2023
medline: 24 5 2023
pubmed: 23 5 2023
entrez: 22 5 2023
Statut: epublish

Résumé

Viscum orientale is a largely used parasitic plant with traditional medicinal properties. They are considered to possess the medicinal properties of host tree which they grow on. It's a least explored plant with ethanopharmacological importance. As a result, the current work aimed to investigate the biological effects of Viscum orientale extract and silver nanoparticles (AgNPs) generated from it. AgNPs synthesized using Viscum orientale plant extract and analysed on time dependent series and was characterized using Ultra Violet UV-visible spectra, Fourier Transform Infrared Spectroscopy FTIR, X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX), Scanning Electron Microscopy (SEM). Further using disc method anti-microbial assay was performed following antioxidation screening using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), reducing power and nitric oxide content and heamgglutination with human blood. On green synthesis using silver, the phyto contituents of plant Viscum orientale effectively reduced silver ions at 3-4 h of continuous stirring to form AgNPs. UV-vis spectra showed a typical peak of AgNPs at 480 nm. The FTIR analysis confirmed the covering of silver layers to bio-compounds of the extract. SEM analysis represented AgNPs as spherical morphologies ranging from 119-222 nm. AgNPs exhibited impressive zone of inhibition against Escherichia coli (8.1 ± 0.3 mm), Staphylococcus aureus (10.3 ± 0.3 mm), Bacillus subtilis (7.3 ± 0.3 mm), Bacillus cereus (8.2 ± 0.3 mm), Salmonella typhi (7.1 ± 0.2 mm). AgNps exhibited efficiency against DPPH at EC Synthesized AgNPs using Viscum orientale water extract displayed versatile biological activity than individual extract. This study has forecasted a new path to explore more on this AgNPs for further research.

Sections du résumé

BACKGROUND BACKGROUND
Viscum orientale is a largely used parasitic plant with traditional medicinal properties. They are considered to possess the medicinal properties of host tree which they grow on. It's a least explored plant with ethanopharmacological importance. As a result, the current work aimed to investigate the biological effects of Viscum orientale extract and silver nanoparticles (AgNPs) generated from it.
METHODS METHODS
AgNPs synthesized using Viscum orientale plant extract and analysed on time dependent series and was characterized using Ultra Violet UV-visible spectra, Fourier Transform Infrared Spectroscopy FTIR, X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX), Scanning Electron Microscopy (SEM). Further using disc method anti-microbial assay was performed following antioxidation screening using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), reducing power and nitric oxide content and heamgglutination with human blood.
RESULTS RESULTS
On green synthesis using silver, the phyto contituents of plant Viscum orientale effectively reduced silver ions at 3-4 h of continuous stirring to form AgNPs. UV-vis spectra showed a typical peak of AgNPs at 480 nm. The FTIR analysis confirmed the covering of silver layers to bio-compounds of the extract. SEM analysis represented AgNPs as spherical morphologies ranging from 119-222 nm. AgNPs exhibited impressive zone of inhibition against Escherichia coli (8.1 ± 0.3 mm), Staphylococcus aureus (10.3 ± 0.3 mm), Bacillus subtilis (7.3 ± 0.3 mm), Bacillus cereus (8.2 ± 0.3 mm), Salmonella typhi (7.1 ± 0.2 mm). AgNps exhibited efficiency against DPPH at EC
CONCLUSION CONCLUSIONS
Synthesized AgNPs using Viscum orientale water extract displayed versatile biological activity than individual extract. This study has forecasted a new path to explore more on this AgNPs for further research.

Identifiants

pubmed: 37217985
doi: 10.1186/s12906-023-03982-1
pii: 10.1186/s12906-023-03982-1
pmc: PMC10204336
doi:

Substances chimiques

Silver 3M4G523W1G
Nitric Oxide 31C4KY9ESH
Anti-Infective Agents 0
Anthelmintics 0
Plant Extracts 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

167

Informations de copyright

© 2023. The Author(s).

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Auteurs

Dugganaboyana Guru Kumar (DG)

Division of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research (Deemed to Be University), Sri Shivarathreeshwara Nagara, Mysuru, 570015, Karnataka, India. gurukumard@jssuni.edu.in.

Raghu Ram Achar (RR)

Division of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research (Deemed to Be University), Sri Shivarathreeshwara Nagara, Mysuru, 570015, Karnataka, India. rracharya@jssuni.edu.in.

Jajur Ramanna Kumar (JR)

Division of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research (Deemed to Be University), Sri Shivarathreeshwara Nagara, Mysuru, 570015, Karnataka, India.

Ganamaedi Amala (G)

Division of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research (Deemed to Be University), Sri Shivarathreeshwara Nagara, Mysuru, 570015, Karnataka, India.

Velliyur Kanniappan Gopalakrishnan (VK)

School of Medicine, Bule Hora University Institute of Health, Bule Hora University, Bule Hora, Ethiopia.

Sushma Pradeep (S)

Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education, Mysore, India.

Ali A Shati (AA)

Biology Department, Faculty of Sciences, King Khalid University, Abha, Saudi Arabia.

Mohammad Y Alfaifi (MY)

Biology Department, Faculty of Sciences, King Khalid University, Abha, Saudi Arabia.

Serag Eldin I Elbehairi (SEI)

Biology Department, Faculty of Sciences, King Khalid University, Abha, Saudi Arabia.

Ekaterina Silina (E)

Department of Hospital Surgery, N.I. Pirogov Russian National Research Medical University (RNRMU), Moscow, 117 997, Russia.

Victor Stupin (V)

Department of Hospital Surgery, N.I. Pirogov Russian National Research Medical University (RNRMU), Moscow, 117 997, Russia.

Natalia Manturova (N)

Department of Hospital Surgery, N.I. Pirogov Russian National Research Medical University (RNRMU), Moscow, 117 997, Russia.

Chandan Shivamallu (C)

Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education, Mysore, India. chandans@jssuni.edu.in.

Shiva Prasad Kollur (SP)

School of Physical Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, 570 026, Karnataka, India. shivachemist@gmail.com.

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Classifications MeSH