Investigating the Role of T-bet
ABCs
DN
SLE
T-bet
age-associated B cells
autoimmunity
Journal
Mediterranean journal of rheumatology
ISSN: 2529-198X
Titre abrégé: Mediterr J Rheumatol
Pays: Greece
ID NLM: 101730166
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
17
01
2022
revised:
23
11
2022
accepted:
05
12
2022
medline:
24
5
2023
pubmed:
24
5
2023
entrez:
24
5
2023
Statut:
epublish
Résumé
Age-associated B cells (ABCs) constitute a B cell subset, defined as CD19 Despite the available data, the functional features of ABCs/DN and their exact role in the pathogenesis of autoimmunity remain elusive. This project focuses on the investigation of the role of ABCs/DN in the pathogenesis of systemic lupus erythematosus (SLE) in humans, as well as the effects that various pharmacological agents may have on these cells. Samples from patients with active SLE will be used to enumerate and immunophenotype - via flow cytometry - the ABCs/DN found in the peripheral blood of the patients. Transcriptomic analysis and functional assays for the cells, both before and after in vitro pharmacological treatments, will also be performed. The results of the study are expected to allow characterization of the pathogenetic role of ABCs/DN in SLE and could probably contribute, following careful association with the clinical state of the patients, towards the discovery and validation of novel prognostic and diagnostic markers of disease.
Sections du résumé
Background
UNASSIGNED
Age-associated B cells (ABCs) constitute a B cell subset, defined as CD19
Aims of the study
UNASSIGNED
Despite the available data, the functional features of ABCs/DN and their exact role in the pathogenesis of autoimmunity remain elusive. This project focuses on the investigation of the role of ABCs/DN in the pathogenesis of systemic lupus erythematosus (SLE) in humans, as well as the effects that various pharmacological agents may have on these cells.
Methods
UNASSIGNED
Samples from patients with active SLE will be used to enumerate and immunophenotype - via flow cytometry - the ABCs/DN found in the peripheral blood of the patients. Transcriptomic analysis and functional assays for the cells, both before and after in vitro pharmacological treatments, will also be performed.
Anticipated benefits
UNASSIGNED
The results of the study are expected to allow characterization of the pathogenetic role of ABCs/DN in SLE and could probably contribute, following careful association with the clinical state of the patients, towards the discovery and validation of novel prognostic and diagnostic markers of disease.
Identifiants
pubmed: 37223601
doi: 10.31138/mjr.34.1.117
pii: MJR-34-1-117
pmc: PMC10201091
doi:
Types de publication
Journal Article
Langues
eng
Pagination
117-120Informations de copyright
© 2023 The Mediterranean Journal of Rheumatology (MJR).
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
J Immunol. 2016 Aug 15;197(4):1023-8
pubmed: 27430719
Int Immunol. 2003 Aug;15(8):937-44
pubmed: 12882831
Blood. 2011 Aug 4;118(5):1294-304
pubmed: 21562046
Lupus Sci Med. 2017 Sep 14;4(1):e000226
pubmed: 29018537
Mech Ageing Dev. 2009 Oct;130(10):681-90
pubmed: 19698733
Blood. 2011 Aug 4;118(5):1305-15
pubmed: 21543762
J Immunol. 2015 Jul 1;195(1):71-9
pubmed: 26034175
Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13248-53
pubmed: 16135562
Drugs. 2011 Dec 24;71(18):2435-44
pubmed: 22141386
Elife. 2019 May 15;8:
pubmed: 31090539
Nat Immunol. 2003 Nov;4(11):1041; author reply 1041
pubmed: 14586416
PLoS One. 2016 Nov 23;11(11):e0166322
pubmed: 27880772
Drugs. 2018 Mar;78(3):355-366
pubmed: 29396833
Nat Immunol. 2012 May 06;13(6):604-11
pubmed: 22561605
Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3216-24
pubmed: 23922396
Lupus. 2013 Oct;22(12):1214-24
pubmed: 24097993
Immunity. 2018 Oct 16;49(4):725-739.e6
pubmed: 30314758
J Exp Med. 2016 May 2;213(5):715-32
pubmed: 27069112
Immunity. 2004 Apr;20(4):477-94
pubmed: 15084276
Cell. 2000 Mar 17;100(6):655-69
pubmed: 10761931
Expert Opin Drug Saf. 2017 Mar;16(3):411-419
pubmed: 27927040
Eur J Immunol. 2019 Jan;49(1):170-178
pubmed: 30353919
Nat Immunol. 2020 Sep;21(9):1094-1106
pubmed: 32747814
J Immunol. 2007 May 15;178(10):6624-33
pubmed: 17475894
Cell Immunol. 2017 Nov;321:35-39
pubmed: 28641866