An archaeal Cas3 protein facilitates rapid recovery from DNA damage.

CRISPR Cas3 DNA-repair archaea haloferax recombination

Journal

microLife
ISSN: 2633-6693
Titre abrégé: Microlife
Pays: England
ID NLM: 9918227365406676

Informations de publication

Date de publication:
2023
Historique:
received: 30 12 2022
accepted: 08 02 2023
medline: 24 5 2023
pubmed: 24 5 2023
entrez: 24 5 2023
Statut: epublish

Résumé

CRISPR-Cas systems provide heritable acquired immunity against viruses to archaea and bacteria. Cas3 is a CRISPR-associated protein that is common to all Type I systems, possesses both nuclease and helicase activities, and is responsible for degradation of invading DNA. Involvement of Cas3 in DNA repair had been suggested in the past, but then set aside when the role of CRISPR-Cas as an adaptive immune system was realized. Here we show that in the model archaeon

Identifiants

pubmed: 37223740
doi: 10.1093/femsml/uqad007
pii: uqad007
pmc: PMC10117719
doi:

Types de publication

Journal Article

Langues

eng

Pagination

uqad007

Informations de copyright

© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.

Déclaration de conflit d'intérêts

None declared.

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Auteurs

Guy Miezner (G)

The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel.

Israela Turgeman-Grott (I)

The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel.

Kelly M Zatopek (KM)

New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938, USA.

Andrew F Gardner (AF)

New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938, USA.

Leah Reshef (L)

The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel.

Deepak K Choudhary (DK)

The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel.

Martina Alstetter (M)

Department of Biology II, Ulm University, 89069 Ulm, Germany.

Thorsten Allers (T)

School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.

Anita Marchfelder (A)

Department of Biology II, Ulm University, 89069 Ulm, Germany.

Uri Gophna (U)

The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel.

Classifications MeSH