Spontaneous Formation of Uniform Cell-Sized Microgels through Water/Water Phase Separation.
aqueous two-phase systems
uniform cell-sized microgels
water/water phase separation
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
revised:
07
05
2023
received:
14
03
2023
medline:
21
9
2023
pubmed:
25
5
2023
entrez:
24
5
2023
Statut:
ppublish
Résumé
In this study, a one-step method is discussed for producing uniform cell-sized microgels using glass capillaries filled with a binary polymer blend of polyethylene glycol (PEG) and gelatin. Upon decreasing temperature, phase separation of the PEG/gelatin blends and gelation of gelatin occur, and then the polymer blend forms linearly aligned, uniformly sized gelatin microgels in the glass capillary. When DNA is added to the polymer solution, gelatin microgels entrapping DNA are spontaneously formed, and the DNA prevents the coalescence of the microdroplets even at temperatures above the melting point. This novel method to form uniform cell-sized microgels may be applicable to other biopolymers. This method is expected to contribute to diverse materials science via biopolymer microgels and biophysics and synthetic biology through cellular models containing biopolymer gels.
Identifiants
pubmed: 37224803
doi: 10.1002/smll.202302193
doi:
Substances chimiques
Microgels
0
Gelatin
9000-70-8
Water
059QF0KO0R
Polyethylene Glycols
3WJQ0SDW1A
Polymers
0
Biopolymers
0
Gels
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2302193Informations de copyright
© 2023 The Authors. Small published by Wiley-VCH GmbH.
Références
S. Herianto, P.-J. Chien, J.-A. A. Ho, H.-L. Tu, Biomater. Adv. 2022, 142, 213156.
S. Hirschi, T. R. Ward, W. P. Meier, D. J. Müller, D. Fotiadis, Chem. Rev. 2022, 122, 16294.
T. Hamada, K. Yoshikawa, Materials 2012, 5, 2292.
P. Stano, Life 2018, 9, 3.
P. Schwille, J. Spatz, K. Landfester, E. Bodenschatz, S. Herminghaus, V. Sourjik, T. J. Erb, P. Bastiaens, R. Lipowsky, A. Hyman, P. Dabrock, J. C. Baret, T. Vidakovic-Koch, P. Bieling, R. Dimova, H. Mutschler, T. Robinson, T. D. Tang, S. Wegner, K. Sundmacher, Angew. Chem., Int. Ed. 2018, 57, 13382.
H. Udono, J. Gong, Y. Sato, M. Takinoue, Adv. Biol. 2022, 7, 2200180.
A. Kato, M. Yanagisawa, Y. T. Sato, K. Fujiwara, K. Yoshikawa, Sci. Rep. 2012, 2, 283.
M. B. C. De Matos, B. S. Miranda, Y. Rizky Nuari, G. Storm, G. Leneweit, R. M. Schiffelers, R. J. Kok, J. Drug Targeting 2019, 27, 681.
Y. Shimane, Y. Kuruma, Front. Bioeng. Biotechnol. 2022, 10, 873854.
K. Takiguchi, M. Negishi, Y. Tanaka-Takiguchi, M. Homma, K. Yoshikawa, Langmuir 2011, 27, 11528.
M. Iqbal, Y. Tao, S. Xie, Y. Zhu, D. Chen, X. Wang, L. Huang, D. Peng, A. Sattar, M. A. Shabbir, H. I. Hussain, S. Ahmed, Z. Yuan, Biol. Proced. Online 2016, 18, 18.
J. R. SooHoo, G. M. Walker, Biomed. Microdevices 2009, 11, 323.
M. Tsukamoto, S. Taira, S. Yamamura, Y. Morita, N. Nagatani, Y. Takamura, E. Tamiya, Analyst 2009, 134, 1994.
Y. Chao, H. C. Shum, Chem. Soc. Rev. 2020, 49, 114.
Q. Ma, Y. Song, W. Sun, J. Cao, H. Yuan, X. Wang, Y. Sun, H. C. Shum, Adv. Sci. 2020, 7, 1903359.
A. T. Rowland, C. D. Keating, Soft Matter 2021, 17, 3688.
R. Mizuuchi, N. Ichihashi, Chem. Commun. 2020, 56, 13453.
H. Sakuta, F. Fujita, T. Hamada, M. Hayashi, K. Takiguchi, K. Tsumoto, K. Yoshikawa, ChemBioChem 2020, 21, 3323.
N. Nakatani, H. Sakuta, M. Hayashi, S. Tanaka, K. Takiguchi, K. Tsumoto, K. Yoshikawa, ChemBioChem 2018, 19, 1370.
K. Tsumoto, H. Sakuta, K. Takiguchi, K. Yoshikawa, Biophys. Rev. 2020, 12, 425.
M. Yanagisawa, Y. Yamashita, S.-A. Mukai, M. Annaka, M. Tokita, J. Mol. Liq. 2014, 200, 2.
M. Yanagisawa, S. Nigorikawa, T. Sakaue, K. Fujiwara, M. Tokita, Proc. Natl. Acad. Sci. USA 2014, 111, 15894.
S. Asakura, F. Oosawa, J. Polym. Sci. 1958, 33, 183.
M. Negishi, M. Ichikawa, M. Nakajima, M. Kojima, T. Fukuda, K. Yoshikawa, Phys. Rev. E 2011, 83, 061921.
E. Y. Basova, F. Foret, Analyst 2015, 140, 22.
S. Lee, J. de Rutte, R. Dimatteo, D. Koo, D. Di Carlo, ACS Nano 2022, 16, 38.
G. Zhang, J. Sun, Int. J. Nanomed. 2021, 16, 7391.
S. Sart, G. Ronteix, S. Jain, G. Amselem, C. N. Baroud, Chem. Rev. 2022, 122, 7061.
P. Zhu, L. Wang, Chem. Rev. 2022, 122, 7010.
K. O. Rojek, M. Ćwiklińska, J. Kuczak, J. Guzowski, Chem. Rev. 2022, 122, 16839.
M. Shono, R. Ito, F. Fujita, H. Sakuta, K. Yoshikawa, Sci. Rep. 2021, 11, 23570.
E. A. G. Jamie, R. P. A. Dullens, D. G. A. L. Aarts, J. Chem. Phys. 2012, 137, 204902.
M. C. Morán, N. Rosell, G. Ruano, M. A. Busquets, M. P. Vinardell, Colloids Surf., B 2015, 134, 156.
V. L. Truong-Le, S. M. Walsh, E. Schweibert, H. Q. Mao, W. B. Guggino, J. T. August, K. W. Leong, Arch. Biochem. Biophys. 1999, 361, 47.
M. Karg, A. Pich, T. Hellweg, T. Hoare, L. Lyon, J. Crassous, D. Suzuki, R. Gumerov, S. Schneider, I. Potemkin, W. Richtering, Langmuir 2019, 35, 6231.
F. Scheffold, Nat. Commun. 2020, 11, 4315.
Q. Feng, D. Li, Q. Li, X. Cao, H. Dong, Bioact. Mater. 2022, 9, 105.
Y. Li, D. Jeong, J. Shin, J. Kim, Comput. Math. Appl. 2013, 65, 102.
P. Benner, J. Heiland, Science Open Research 2015, 1.
Z. Gan, H. Liu, Y. Wang, T. Tao, M. Zhao, J. Qin, Adv. Mater. Technol. 2023, 8, 2201102.
H. Kikuchi, K. Nose, Y. Yoshikawa, K. Yoshikawa, Chem. Phys. Lett. 2018, 701, 81.