Kielin/chordin-like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein-2.

BMP C2C12 myoblasts KCP (kielim/chordin-like protein) osteoarthritis osteoblasts

Journal

FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716

Informations de publication

Date de publication:
07 2023
Historique:
revised: 11 05 2023
received: 22 03 2023
accepted: 24 05 2023
medline: 4 7 2023
pubmed: 25 5 2023
entrez: 25 5 2023
Statut: ppublish

Résumé

Bone morphogenetic proteins (BMPs) play a key role in embryonic differentiation for osteoblast and bone formation. Kielin/chordin-like protein (Kcp) is known to enhance the effects of BMP signaling. Here, we present ALP activity, gene expression, and calcification data demonstrating that Kcp affects the differentiation of C2C12 myoblasts into osteoblasts. We report that the presence of Kcp enhances the ability of BMP-2 to induce the differentiation of C2C12 myoblasts into osteoblasts. Additionally, BMP-2-mediated stimulation of phosphorylated Smad1/5 was apparently enhanced in the presence of Kcp. The present findings may contribute to progression toward the clinical use of BMPs for treatment of bone fracture, osteoarthritis, and other similar conditions.

Identifiants

pubmed: 37226930
doi: 10.1002/2211-5463.13652
pmc: PMC10315795
doi:

Substances chimiques

chordin 93586-27-7
Bone Morphogenetic Proteins 0
Glycoproteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1357-1364

Informations de copyright

© 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

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Auteurs

Kei Nagasaki (K)

Department of Biochemistry, School of Dentistry, Showa University, Shinagawa-ku, Japan.
Department of Orthopedic Surgery, School of Medicine, Showa University, Shinagawa-ku, Japan.
Department of Pharmacology, School of Medicine, Showa University, Shinagawa-ku, Japan.

Atsushi Yamada (A)

Department of Biochemistry, School of Dentistry, Showa University, Shinagawa-ku, Japan.

Kiyohito Sasa (K)

Department of Biochemistry, School of Dentistry, Showa University, Shinagawa-ku, Japan.

Ryutaro Kamijo (R)

Department of Biochemistry, School of Dentistry, Showa University, Shinagawa-ku, Japan.

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Classifications MeSH