Transcriptional dynamics of delaminating neuroblasts in the mouse otic vesicle.

Mice Animals Cochlea Neural Stem Cells / metabolism Neurons Gene Expression Regulation, Developmental
CP: Neuroscience cochlea cochleovestibular ganglion neurogenesis otic neuroblasts otic placode otic vesicle scRNA-seq vestibular

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
27 06 2023
Historique:
received: 18 05 2022
revised: 23 02 2023
accepted: 04 05 2023
medline: 4 10 2023
pubmed: 25 5 2023
entrez: 25 5 2023
Statut: ppublish

Résumé

An abundance of research has recently highlighted the susceptibility of cochleovestibular ganglion (CVG) neurons to noise damage and aging in the adult cochlea, resulting in hearing deficits. Furthering our understanding of the transcriptional cascades that contribute to CVG development may provide insight into how these cells can be regenerated to treat inner ear dysfunction. Here we perform a high-depth single-cell RNA sequencing analysis of the E10.5 otic vesicle and its surrounding tissues, including CVG precursor neuroblasts and emerging CVG neurons. Clustering and trajectory analysis of otic-lineage cells reveals otic markers and the changes in gene expression that occur from neuroblast delamination toward the development of the CVG. This dataset provides a valuable resource for further identifying the mechanisms associated with CVG development from neurosensory competent cells within the otic vesicle.

Identifiants

DOI: 10.1016/j.celrep.2023.112545 PMID: 37227818 PMC: PMC10592509
pubmed: 37227818
pii: S2211-1247(23)00556-9
doi: 10.1016/j.celrep.2023.112545
pmc: PMC10592509
mid: NIHMS1912765
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

112545

Subventions

Organisme : NIDCD NIH HHS
ID : R01 DC017689
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC019370
Pays : United States
Organisme : NIDCD NIH HHS
ID : R21 DC019910
Pays : United States
Organisme : NIDCD NIH HHS
ID : T32 DC015209
Pays : United States

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests S.H. is a paid consultant of Pipeline Therapeutics.

Références

Cell Tissue Res. 2010 Jul;341(1):95-110
pubmed: 20512592
Nat Commun. 2015 Oct 15;6:8557
pubmed: 26469390
J Neurosci. 1998 May 1;18(9):3327-35
pubmed: 9547240
Dev Dyn. 2006 Sep;235(9):2470-82
pubmed: 16691564
J Biol Chem. 2012 Mar 2;287(10):7573-81
pubmed: 22235123
Neurosci Lett. 2011 Jun 22;497(2):74-9
pubmed: 21540077
PLoS Genet. 2011 Sep;7(9):e1002309
pubmed: 21980309
Nat Methods. 2021 Aug;18(8):843-844
pubmed: 34172972
Int J Mol Sci. 2021 Apr 18;22(8):
pubmed: 33919542
Development. 2003 Sep;130(17):3989-4000
pubmed: 12874121
Nat Immunol. 2019 Feb;20(2):163-172
pubmed: 30643263
Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13552-13561
pubmed: 32482884
Front Cell Dev Biol. 2020 Sep 03;8:567537
pubmed: 33015061
Cell. 2018 Jul 26;174(3):536-548.e21
pubmed: 29961578
Cancer Lett. 2011 Jan 1;300(1):66-78
pubmed: 20980099
Development. 2011 Dec;138(24):5403-14
pubmed: 22110056
Cell Growth Differ. 1992 Mar;3(3):143-8
pubmed: 1633105
Cell Rep. 2021 Jan 19;34(3):108646
pubmed: 33472062
Cell Rep. 2018 Jun 5;23(10):2901-2914.e13
pubmed: 29874578
Nat Commun. 2020 May 13;11(1):2389
pubmed: 32404924
Elife. 2017 Jul 25;6:
pubmed: 28742024
Development. 2004 May;131(9):2125-35
pubmed: 15105372
Biol Open. 2018 Nov 19;7(11):
pubmed: 30455179
Laryngoscope. 2021 Sep;131 Suppl 5:S1-S16
pubmed: 32579737
Nat Biotechnol. 2015 Feb;33(2):155-60
pubmed: 25599176
Front Cell Dev Biol. 2021 Oct 21;9:730042
pubmed: 34746126
Dev Dyn. 2021 Oct;250(10):1450-1462
pubmed: 33715274
Dev Dyn. 2013 Mar;242(3):281-90
pubmed: 23233200
J Invest Dermatol. 1997 Mar;108(3):307-12
pubmed: 9036930
Dev Biol. 2014 Jul 15;391(2):158-69
pubmed: 24780628
Bioinformatics. 2014 Feb 15;30(4):523-30
pubmed: 24336805
Genes Dev. 2016 Oct 15;30(20):2310-2324
pubmed: 27807034
Cell. 2018 Aug 23;174(5):1229-1246.e17
pubmed: 30078709
Cold Spring Harb Perspect Biol. 2012 Aug 01;4(8):a008409
pubmed: 22855724
Nat Commun. 2018 Sep 12;9(1):3691
pubmed: 30209249
Genesis. 2004 Apr;38(4):195-9
pubmed: 15083520
Front Cell Neurosci. 2015 Mar 19;9:79
pubmed: 25852475
Dev Cell. 2017 Nov 6;43(3):318-331.e5
pubmed: 29107558
Nat Protoc. 2015 Mar;10(3):459-474
pubmed: 25675210
Development. 2021 Apr 15;148(8):
pubmed: 33795231
Cell Mol Life Sci. 2018 Apr;75(7):1179-1190
pubmed: 29103147
PLoS Genet. 2011 Sep;7(9):e1002307
pubmed: 21980308
Hum Mol Genet. 2011 Apr 1;20(7):1262-73
pubmed: 21227998
Cell Rep. 2021 Jul 13;36(2):109358
pubmed: 34260939
Front Cell Dev Biol. 2022 Feb 17;10:845461
pubmed: 35252209
Dev Biol. 2008 Oct 1;322(1):109-20
pubmed: 18674529
Bioinformatics. 2020 Apr 15;36(8):2628-2629
pubmed: 31882993
Cell Cycle. 2015;14(4):481-7
pubmed: 25607528
J Neurosci. 2019 Jul 3;39(27):5284-5298
pubmed: 31085606
Nat Biotechnol. 2018 Jun;36(5):421-427
pubmed: 29608177
Prog Neurobiol. 2011 Apr;93(4):488-508
pubmed: 21232575
Cell Rep. 2022 Mar 22;38(12):110542
pubmed: 35320729
Dev Biol. 2000 Jun 15;222(2):296-306
pubmed: 10837119
Cells. 2019 Sep 20;8(10):
pubmed: 31547193
Cell. 2014 May 8;157(4):964-78
pubmed: 24768691
Cell Rep. 2018 Aug 14;24(7):1865-1879.e9
pubmed: 30110642
Curr Cancer Drug Targets. 2021;21(9):749-767
pubmed: 34077345
Nat Rev Cancer. 2006 Feb;6(2):99-106
pubmed: 16491069
Development. 2007 Dec;134(24):4405-15
pubmed: 18039969
Oral Oncol. 2013 Jan;49(1):34-41
pubmed: 22892238
Curr Top Dev Biol. 1998;36:223-43
pubmed: 9342531
J Biol Chem. 2008 May 30;283(22):14910-4
pubmed: 18411277
Gene. 2018 Aug 5;666:145-157
pubmed: 29730428
Mech Dev. 2015 Nov;138 Pt 3:233-45
pubmed: 26545349
J Comp Neurol. 2004 Sep 27;477(4):412-21
pubmed: 15329890
Front Cell Dev Biol. 2022 Feb 14;9:820105
pubmed: 35237613
Int J Mol Med. 2018 Oct;42(4):1771-1785
pubmed: 30015855
Dev Biol. 2014 Jan 15;385(2):200-10
pubmed: 24252775
Mol Cancer. 2016 Feb 24;15:18
pubmed: 26905733
J Embryol Exp Morphol. 1958 Mar;6(1):105-16
pubmed: 13539273
Neuron. 1999 Jun;23(2):257-71
pubmed: 10399933
J Comp Neurol. 1983 Apr 20;215(4):359-69
pubmed: 6863589
Cell Adh Migr. 2009 Jan-Mar;3(1):29-35
pubmed: 19372758
J Neurosci. 2012 Nov 28;32(48):17172-85
pubmed: 23197710
Curr Opin Neurobiol. 2002 Feb;12(1):35-42
pubmed: 11861162
Development. 1997 Aug;124(15):2961-72
pubmed: 9247338
Dev Dyn. 2007 Apr;236(4):961-70
pubmed: 17330888
Neuron. 1999 Apr;22(4):677-91
pubmed: 10230789
Cell. 2018 Aug 23;174(5):1247-1263.e15
pubmed: 30078710

Auteurs

Maggie S Matern (MS)

Department of Otolaryngology Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA; Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA.

Robert Durruthy-Durruthy (R)

Department of Otolaryngology Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA; Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA.

Onur Birol (O)

Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.

Spyros Darmanis (S)

Departments of Bioengineering and Applied Physics and Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.

Mirko Scheibinger (M)

Department of Otolaryngology Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA; Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA.

Andrew K Groves (AK)

Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.

Stefan Heller (S)

Department of Otolaryngology Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA; Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA. Electronic address: hellers@stanford.edu.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Transcriptional dynamics of delaminating...
questionsmedicales.fr › Eucaryotes › Animaux › Transcriptional dynamics of delaminating...
questionsmedicales.fr › Organes des sens › Oreille › Oreille interne › Cochlée › Transcriptional dynamics of delaminating...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches neurales › Transcriptional dynamics of delaminating...
questionsmedicales.fr › Cellules › Neurones › Transcriptional dynamics of delaminating...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes au cours du développement › Transcriptional dynamics of delaminating...