Rapid Multiplex RT-PCR for Influenza A and B by Genesoc
GeneSoC®
antigen detection test
influenza A
influenza B
realtime-PCR
Journal
Yonago acta medica
ISSN: 0513-5710
Titre abrégé: Yonago Acta Med
Pays: Japan
ID NLM: 0414002
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
received:
07
03
2023
accepted:
03
04
2023
medline:
25
5
2023
pubmed:
25
5
2023
entrez:
25
5
2023
Statut:
epublish
Résumé
Rapid antigen tests are widely used to diagnose influenza. However, despite their simplicity and short turnover time, the sensitivity of these tests is relatively low, and molecular tests with greater sensitivity are being sought. In this study, we developed and clinically evaluated a protocol for the rapid multiplex testing of influenza A and B, using a rapid real-time PCR system, GeneSoC The specificity of the developed assay was validated using cultured viral strains of influenza A/B, human metapneumovirus, and respiratory syncytial virus. Analytical sensitivity was evaluated using serially diluted RNA synthesized via The GeneSoC The microfluidic real-time PCR system, GeneSoC
Sections du résumé
Background
UNASSIGNED
Rapid antigen tests are widely used to diagnose influenza. However, despite their simplicity and short turnover time, the sensitivity of these tests is relatively low, and molecular tests with greater sensitivity are being sought. In this study, we developed and clinically evaluated a protocol for the rapid multiplex testing of influenza A and B, using a rapid real-time PCR system, GeneSoC
Methods
UNASSIGNED
The specificity of the developed assay was validated using cultured viral strains of influenza A/B, human metapneumovirus, and respiratory syncytial virus. Analytical sensitivity was evaluated using serially diluted RNA synthesized via
Results
UNASSIGNED
The GeneSoC
Conclusion
UNASSIGNED
The microfluidic real-time PCR system, GeneSoC
Identifiants
pubmed: 37229367
doi: 10.33160/yam.2023.05.007
pii: 2023.05.007
pmc: PMC10203650
doi:
Types de publication
Journal Article
Langues
eng
Pagination
223-231Informations de copyright
©2023 Tottori University Medical Press.
Déclaration de conflit d'intérêts
Conflict of interest: H. Chikumi was provided the study materials (GeneSoC® chips), and is participating in the other joint research project of which theme is different from this study with Kyorin Pharmaceutical Co., Ltd. Kryoin Pharmaceutical Co., Ltd. had no role in the conduct of the study, data collection, data interpretation or preparation of the report.
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