Exploring the Enteric Virome of Cats with Acute Gastroenteritis.

ONT sequencing PCRs and RT-PCRs cats gastroenteritis viruses

Journal

Veterinary sciences
ISSN: 2306-7381
Titre abrégé: Vet Sci
Pays: Switzerland
ID NLM: 101680127

Informations de publication

Date de publication:
18 May 2023
Historique:
received: 25 04 2023
revised: 09 05 2023
accepted: 17 05 2023
medline: 26 5 2023
pubmed: 26 5 2023
entrez: 26 5 2023
Statut: epublish

Résumé

Viruses are a major cause of acute gastroenteritis (AGE) in cats, chiefly in younger animals. Enteric specimens collected from 29 cats with acute enteritis and 33 non-diarrhoeic cats were screened in PCRs and reverse transcription (RT) PCR for a large panel of enteric viruses, including also orphan viruses of recent identification. At least one viral species, including feline panleukopenia virus (FPV), feline enteric coronavirus (FCoV), feline chaphamaparvovirus, calicivirus (vesivirus and novovirus), feline kobuvirus, feline sakobuvirus A and Lyon IARC polyomaviruses, was detected in 66.1% of the samples.. Co-infections were mainly accounted for by FPV and FCoV and were detected in 24.2% of the samples. The virome composition was further assessed in eight diarrhoeic samples, through the construction of sequencing libraries using a sequence-independent single-primer amplification (SISPA) protocol. The libraries were sequenced on Oxford Nanopore Technologies sequencing platform. A total of 41 contigs (>100 nt) were detected from seven viral families infecting mammals, included

Identifiants

pubmed: 37235445
pii: vetsci10050362
doi: 10.3390/vetsci10050362
pmc: PMC10224047
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Federica Di Profio (F)

Department of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, Italy.

Vittorio Sarchese (V)

Department of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, Italy.

Paola Fruci (P)

Department of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, Italy.

Giovanni Aste (G)

Department of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, Italy.

Vito Martella (V)

Department of Veterinary Medicine, Università Aldo Moro di Bari, 70010 Valenzano, Italy.

Andrea Palombieri (A)

Department of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, Italy.

Barbara Di Martino (B)

Department of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, Italy.

Classifications MeSH