Understanding of arthrofibrosis: New explorative insights into extracellular matrix remodeling of synovial fibroblasts.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
07
10
2022
accepted:
14
05
2023
medline:
29
5
2023
pubmed:
26
5
2023
entrez:
26
5
2023
Statut:
epublish
Résumé
Arthrofibrosis following total knee arthroplasty is a fibroproliferative joint disorder marked by dysregulated biosynthesis of extracellular matrix proteins, such as collagens and proteoglycans. The underlying cellular events remain incompletely understood. Myofibroblasts are highly contractile matrix-producing cells characterized by increased alpha-smooth muscle actin expression and xylosyltransferase-I (XT-I) secretion. Human XT-I has been identified as a key mediator of arthrofibrotic remodeling. Primary fibroblasts from patients with arthrofibrosis provide a useful in vitro model to identify and characterize disease regulators and potential therapeutic targets. This study aims at characterizing primary synovial fibroblasts from arthrofibrotic tissues (AFib) regarding their molecular and cellular phenotype by utilizing myofibroblast cell culture models. Compared to synovial control fibroblasts (CF), AFib are marked by enhanced cell contractility and a higher XT secretion rate, demonstrating an increased fibroblast-to-myofibroblast transition rate during arthrofibrosis. Histochemical assays and quantitative gene expression analysis confirmed higher collagen and proteoglycan expression and accumulation in AFib compared to CF. Furthermore, fibrosis-based gene expression profiling identified novel modifier genes in the context of arthrofibrosis remodeling. In summary, this study revealed a unique profibrotic phenotype in AFib that resembles some traits of other fibroproliferative diseases and can be used for the future development of therapeutic interventions.
Identifiants
pubmed: 37235555
doi: 10.1371/journal.pone.0286334
pii: PONE-D-22-27716
pmc: PMC10218749
doi:
Substances chimiques
Collagen
9007-34-5
Actins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0286334Informations de copyright
Copyright: © 2023 Ly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Acta Derm Venereol. 2000 Jan-Feb;80(1):60-1
pubmed: 10721843
Biosci Rep. 2016 Jul 15;36(4):
pubmed: 27247426
Nat Cell Biol. 2002 Aug;4(8):599-604
pubmed: 12134160
Histochemie. 1965 Oct 1;5(3):221-33
pubmed: 4223499
Bone Res. 2019 Mar 26;7:9
pubmed: 30937213
J Clin Invest. 2018 Jan 2;128(1):45-53
pubmed: 29293088
Unfallchirurg. 2008 Feb;111(2):79-84
pubmed: 18239901
JBJS Rev. 2020 Jul;8(7):e1900223
pubmed: 32618740
J Allergy Clin Immunol. 2006 Jan;117(1):103-10
pubmed: 16387592
Bone Joint Res. 2018 May 05;7(3):213-222
pubmed: 29922438
Histochem Cell Biol. 1999 Oct;112(4):271-6
pubmed: 10550611
Anal Biochem. 1984 Mar;137(2):505-16
pubmed: 6731831
Sci Rep. 2015 Nov 10;5:16466
pubmed: 26553966
J Orthop Surg Res. 2020 Jan 23;15(1):27
pubmed: 31973761
Mol Biol Cell. 2001 Sep;12(9):2730-41
pubmed: 11553712
Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12877-82
pubmed: 12297622
Biochem Biophys Res Commun. 2021 Apr 16;549:34-39
pubmed: 33662666
Invest Ophthalmol Vis Sci. 2011 Nov 01;52(12):8549-57
pubmed: 21989726
Am J Pathol. 2012 Apr;180(4):1340-55
pubmed: 22387320
Front Biosci (Landmark Ed). 2009 Jun 01;14(13):4992-5012
pubmed: 19482601
Am J Respir Crit Care Med. 2008 Feb 1;177(3):321-9
pubmed: 17975199
Matrix Biol. 2018 Aug;68-69:28-43
pubmed: 29288716
Annu Rev Immunol. 1998;16:137-61
pubmed: 9597127
Sci Rep. 2015 Jul 28;5:12537
pubmed: 26219087
Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9448-53
pubmed: 22566629
Connect Tissue Res. 2019 Jan;60(1):21-28
pubmed: 30173570
Chest. 2007 Oct;132(4):1311-21
pubmed: 17934117
J Hepatol. 2015 Apr;62(1 Suppl):S15-24
pubmed: 25920084
J Arthroplasty. 2017 Aug;32(8):2604-2611
pubmed: 28285897
Sci Rep. 2015 Nov 10;5:16469
pubmed: 26553967
Arch Orthop Trauma Surg. 2004 Nov;124(9):585-91
pubmed: 15378321
PLoS One. 2022 Jan 28;17(1):e0262549
pubmed: 35089951
J Scleroderma Relat Disord. 2017 May-Aug;2(2):69-134
pubmed: 29270465
Biochem Biophys Res Commun. 2013 Jul 5;436(3):449-54
pubmed: 23747722
Am J Physiol Gastrointest Liver Physiol. 2015 May 15;308(10):G807-30
pubmed: 25767261
Genomics. 2020 Jul;112(4):2703-2712
pubmed: 32145378
Front Biosci. 1997 Jan 01;2:d12-26
pubmed: 9159205
Matrix Biol. 2018 Aug;68-69:1-7
pubmed: 29679639
J Histochem Cytochem. 1961 Sep;9:572-7
pubmed: 13906980
Eur Respir J. 2001 Aug;18(2):347-56
pubmed: 11529295
J Clin Invest. 2011 Jul;121(7):2863-75
pubmed: 21646718
Biochem Biophys Res Commun. 2020 Mar 19;523(4):1001-1006
pubmed: 31973816
J Biol Chem. 2007 Sep 7;282(36):26441-9
pubmed: 17635914
Cells Tissues Organs. 2008;187(3):165-76
pubmed: 18042973
Lab Invest. 2008 Oct;88(10):1090-100
pubmed: 18663351
Oncotarget. 2017 Mar 28;8(13):20741-20750
pubmed: 28186964
Lab Invest. 2010 Nov;90(11):1615-27
pubmed: 20697373
J Orthop Surg Res. 2021 Dec 20;16(1):726
pubmed: 34930351
J Invest Dermatol. 1999 Jun;112(6):919-24
pubmed: 10383739
PLoS Genet. 2006 Jul;2(7):e119
pubmed: 16895450
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
Mol Hum Reprod. 2002 Dec;8(12):1079-86
pubmed: 12468640
Respir Res. 2016 Mar 04;17:23
pubmed: 26944412
Glia. 2007 Aug 15;55(11):1145-55
pubmed: 17597120
Sci Rep. 2018 Dec 12;8(1):17779
pubmed: 30542210
Bone Joint Res. 2014 Mar 26;3(3):82-8
pubmed: 24671942
J Mol Cell Cardiol. 2018 Aug;121:205-211
pubmed: 30040954
Adv Drug Deliv Rev. 2017 Nov 1;121:101-116
pubmed: 28720422
Biomolecules. 2020 Apr 14;10(4):
pubmed: 32295230
Eur J Hum Genet. 2014 Jun;22(6):726-33
pubmed: 24129431
J Rheumatol. 1996 Aug;23(8):1398-406
pubmed: 8856620
Matrix Biol. 2018 Aug;68-69:122-149
pubmed: 29458139
Front Pharmacol. 2014 May 27;5:123
pubmed: 24904424
J Dermatol Sci. 2008 Feb;49(2):103-13
pubmed: 17628443
Biomolecules. 2020 Oct 21;10(10):
pubmed: 33096778
Pharmaceuticals (Basel). 2018 Feb 27;11(1):
pubmed: 29495527
Growth Factors. 2011 Oct;29(5):196-202
pubmed: 21740331
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Matrix Biol. 2015 Sep;47:54-65
pubmed: 25960420
J Cell Sci. 2020 Jun 15;133(12):
pubmed: 32540905
Front Biosci (Landmark Ed). 2013 Jun 01;18(4):1407-34
pubmed: 23747893
Gene. 2017 Apr 30;610:15-23
pubmed: 28167142
Exp Cell Res. 2017 Nov 15;360(2):153-162
pubmed: 28882457
Cell. 2000 Oct 13;103(2):295-309
pubmed: 11057902
J Cardiovasc Dev Dis. 2019 Sep 23;6(4):
pubmed: 31547598