NAT10, an RNA Cytidine Acetyltransferase, Regulates Ferroptosis in Cancer Cells.
NAT10
RNA acetylation
ROS
SLC7A11
ferroptosis
Journal
Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981
Informations de publication
Date de publication:
18 May 2023
18 May 2023
Historique:
received:
27
02
2023
revised:
27
04
2023
accepted:
15
05
2023
medline:
27
5
2023
pubmed:
27
5
2023
entrez:
27
5
2023
Statut:
epublish
Résumé
Recently, we reported that N-acetyltransferase 10 (NAT10) regulates fatty acid metabolism through ac4C-dependent RNA modification of key genes in cancer cells. During this work, we noticed ferroptosis as one of the most negatively enriched pathways among other pathways in NAT10-depleted cancer cells. In the current work, we explore the possibility of whether NAT10 acts as an epitranscriptomic regulator of the ferroptosis pathway in cancer cells. Global ac4C levels and expression of NAT10 with other ferroptosis-related genes were assessed via dotblot and RT-qPCR, respectively. Flow cytometry and biochemical analysis were used to assess oxidative stress and ferroptosis features. The ac4C-mediated mRNA stability was conducted using RIP-PCR and mRNA stability assay. Metabolites were profiled using LC-MS/MS. Our results showed significant downregulation in expression of essential genes related to ferroptosis, namely
Identifiants
pubmed: 37237981
pii: antiox12051116
doi: 10.3390/antiox12051116
pmc: PMC10215874
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : The Deputyship for Research and Innovation, Ministry of Education in Saudi Arabia
ID : IFPRC-153-130-2020
Références
Transl Cancer Res. 2022 Aug;11(8):2688-2699
pubmed: 36093519
Oncol Rep. 2020 Jan;43(1):147-158
pubmed: 31789401
Front Pharmacol. 2022 Aug 29;13:910292
pubmed: 36105219
Cell Commun Signal. 2022 Aug 19;20(1):127
pubmed: 35986334
Front Oncol. 2022 Aug 01;12:967811
pubmed: 35978804
Signal Transduct Target Ther. 2022 Apr 22;7(1):128
pubmed: 35459217
Mol Cell Biochem. 2007 Jun;300(1-2):249-58
pubmed: 17180247
Cell Death Dis. 2022 Aug 13;13(8):704
pubmed: 35963845
Food Chem Toxicol. 2022 Aug;166:113255
pubmed: 35772596
Sci Rep. 2021 Mar 15;11(1):6051
pubmed: 33723305
ACS Appl Mater Interfaces. 2022 Sep 28;14(38):42791-42803
pubmed: 36112832
Science. 2014 May 2;344(6183):527-32
pubmed: 24786082
Cell. 2018 Dec 13;175(7):1872-1886.e24
pubmed: 30449621
Transl Oncol. 2020 Aug;13(8):100783
pubmed: 32428852
J Cell Biochem. 2021 Dec;122(12):1936-1945
pubmed: 34605570
J Biol Chem. 2017 Aug 25;292(34):14240-14249
pubmed: 28630042
Bio Protoc. 2018 Nov 05;8(21):e3072
pubmed: 34532533
Acta Pharm Sin B. 2022 Aug;12(8):3313-3325
pubmed: 35967285
Cells. 2022 Jun 27;11(13):
pubmed: 35805124
Nat Chem Biol. 2017 Jan;13(1):91-98
pubmed: 27842070
Nucleic Acids Res. 2020 Apr 17;48(7):3638-3656
pubmed: 32112098
Proc Natl Acad Sci U S A. 2020 Dec 22;117(51):32433-32442
pubmed: 33288688
Nutr Cancer. 2021;73(3):460-472
pubmed: 32286088
Oxid Med Cell Longev. 2022 Jun 16;2022:3192607
pubmed: 35757509
Oxid Med Cell Longev. 2022 Jul 19;2022:5361241
pubmed: 35915609
J Mol Neurosci. 2022 Sep;72(9):1797-1808
pubmed: 35727524
Adv Cancer Res. 2018;140:191-215
pubmed: 30060809
Antioxidants (Basel). 2022 Jul 31;11(8):
pubmed: 36009223
Cell Death Dis. 2020 Oct 27;11(10):922
pubmed: 33110056
Prog Lipid Res. 2014 Jul;55:1-16
pubmed: 24769127
Int J Mol Sci. 2020 Oct 28;21(21):
pubmed: 33126604
Sci Rep. 2022 Jul 14;12(1):11996
pubmed: 35835852
Biochem Biophys Res Commun. 2022 Oct 1;623:1-8
pubmed: 35868067
Clin Transl Med. 2022 Sep;12(9):e1045
pubmed: 36149760
J Allergy Clin Immunol. 2021 May;147(5):1936-1948.e9
pubmed: 33130063
Biochem Biophys Res Commun. 2017 Jan 29;483(1):624-629
pubmed: 27993683
J Med Chem. 2017 Oct 26;60(20):8661-8666
pubmed: 28921987
Cell Chem Biol. 2022 Sep 15;29(9):1363-1365
pubmed: 36113403
Kidney Int. 2022 Dec;102(6):1259-1275
pubmed: 36063875
Front Oncol. 2020 Dec 17;10:598107
pubmed: 33425753
Diabetes Metab Syndr Obes. 2020 Sep 25;13:3337-3348
pubmed: 33061495
Commun Biol. 2022 Sep 6;5(1):916
pubmed: 36068299