Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance.
CRISPR/Cas9
DNA methylation
DNMT1
DNMT3A
TET1
dCas9
demethylation
epigenetic editing
steric hindrance
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
22 Apr 2023
22 Apr 2023
Historique:
received:
31
03
2023
revised:
19
04
2023
accepted:
20
04
2023
medline:
27
5
2023
pubmed:
27
5
2023
entrez:
27
5
2023
Statut:
epublish
Résumé
As advances in genome engineering inch the technology towards wider clinical use-slowed by technical and ethical hurdles-a newer offshoot, termed "epigenome engineering", offers the ability to correct disease-causing changes in the DNA without changing its sequence and, thus, without some of the unfavorable correlates of doing so. In this review, we note some of the shortcomings of epigenetic editing technology-specifically the risks involved in the introduction of epigenetic enzymes-and highlight an alternative epigenetic editing strategy using physical occlusion to modify epigenetic marks at target sites without a requirement for any epigenetic enzyme. This may prove to be a safer alternative for more specific epigenetic editing.
Identifiants
pubmed: 37238909
pii: biomedicines11051238
doi: 10.3390/biomedicines11051238
pmc: PMC10215309
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Subventions
Organisme : CIHR
ID : PJT159583
Pays : Canada
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