Eosinophil Granule Proteins Involvement in Acute Appendicitis-An Allergic Disease?


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
22 May 2023
Historique:
received: 15 04 2023
revised: 11 05 2023
accepted: 13 05 2023
medline: 29 5 2023
pubmed: 27 5 2023
entrez: 27 5 2023
Statut: epublish

Résumé

Several pieces of evidence point to an allergic component as a trigger of acute appendicitis. As the Th2 immune response is characterized by eosinophil mobilization to the target organ and release of their cationic granule proteins, it is reasonable to investigate if the degranulation of eosinophils could be associated with the local injury. The primary aim of this study is to evaluate the participation of eosinophils granules proteins in acute appendicitis, both at local and systemic levels and the secondary aim is to evaluate the diagnostic accuracy of eosinophils granules proteins for the detection of acute appendicitis, as well as for distinguishing between complicated and uncomplicated acute appendicitis. Eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP) and eosinophil peroxidase (EP) are the most well-known eosinophil granule proteins. From August 2021 to April 2022, we present a prospective single-center study to evaluate the EDN, ECP, and EP concentrations simultaneously in appendicular lavage fluid (ALF) and the serum of 22 patients with acute phlegmonous appendicitis (APA), 24 with acute gangrenous appendicitis (AGA), and 14 normal controls. Concerning EDN, no differences were found between groups. ECP concentrations in ALF and serum were significantly higher in the histologically confirmed acute appendicitis compared to the control groups (

Identifiants

pubmed: 37240441
pii: ijms24109091
doi: 10.3390/ijms24109091
pmc: PMC10219475
pii:
doi:

Substances chimiques

Eosinophil Granule Proteins 0
Blood Proteins 0
Ribonucleases EC 3.1.-
Eosinophil-Derived Neurotoxin EC 3.1.-
Eosinophil Cationic Protein EC 3.1.27.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Déclaration de conflit d'intérêts

The authors declare no conflicts of interest.

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Auteurs

Nuno Carvalho (N)

Serviço Cirurgia Geral, Hospital Garcia de Orta, 2805-267 Almada, Portugal.
Faculdade Medicina, Universidade Lisboa, 1649-028 Lisboa, Portugal.

Elisabete Carolino (E)

H&TRC-Health & Technology Research Center, ESTeSL-Escola Superior de Tecnologia da Saúde, Instituto Politécnico de Lisboa, 1549-020 Lisboa, Portugal.

Hélder Coelho (H)

Serviço de Anatomia Patológica, Hospital Garcia de Orta, 2805-267 Almada, Portugal.

Ana Lúcia Barreira (AL)

Serviço Cirurgia Geral, Hospital Garcia de Orta, 2805-267 Almada, Portugal.

Luísa Moreira (L)

Serviço de Urologia, Hospital Garcia de Orta, 2805-267 Almada, Portugal.

Margarida André (M)

Serviço de Urologia, Hospital Garcia de Orta, 2805-267 Almada, Portugal.

Susana Henriques (S)

Serviço Cirurgia Geral, Hospital Garcia de Orta, 2805-267 Almada, Portugal.

Carlos Cardoso (C)

Dr. Joaquim Chaves Laboratório de Análises Clínicas, 1495-068 Alges, Portugal.

Luis Moita (L)

Innate Immunity and Inflammation Lab, Instituto Gulbenkian de Ciência Oeiras, 2780-156 Oeiras, Portugal.
Instituto de Histologia e Biologia do Desenvolvimento, Faculdade Medicina, Universidade Lisboa, 1649-028 Lisboa, Portugal.

Paulo Matos Costa (PM)

Serviço Cirurgia Geral, Hospital Garcia de Orta, 2805-267 Almada, Portugal.
Faculdade Medicina, Universidade Lisboa, 1649-028 Lisboa, Portugal.

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Classifications MeSH