Working with Immortalized Hepatic Stellate Cell Lines.
Cell banking
Cell line
Contamination
Cryo-conservation
Freezing
Marker genes
Media
Misidentification
Risk assessment
Supplements
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
31
5
2023
pubmed:
29
5
2023
entrez:
29
5
2023
Statut:
ppublish
Résumé
Hepatic stellate cells (HSCs) are the major cellular source of extracellular matrix production in the liver. Therefore, this cell population has received considerable attention in studies investigating fundamental features of hepatic fibrosis. However, the limited supply and ever-increasing demand for these cells, combined with the additional tightening of formal standards in animal welfare policy, make working with these primary cells increasingly difficult. Moreover, researchers working in biomedical research are challenged to implement the 3R principle of "replacement," "reduction," and "refinement" in their work. This principle, originally proposed in 1959 by William M. S. Russell and Rex L. Burch, is now widely endorsed by legislators and regulatory bodies in many countries as a roadmap to tackle the ethical dilemma associated with animal experimentation. As such, working with immortalized HSC lines is a good alternative to limit the number of animals and their suffering in biomedical research. This article summarizes issues that need to be considered when working with established HSC cell lines and provides general guidelines for the maintenance and storage of HSC lines from mouse, rat, and humans.
Identifiants
pubmed: 37247058
doi: 10.1007/978-1-0716-3207-9_8
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
129-162Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK128289
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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