The Expression and Role of NADPH Oxidase 2 in Colon Cancer.

Humans Colonic Neoplasms / genetics NADPH Oxidase 2 / genetics NADPH Oxidases / genetics Prognosis Reactive Oxygen Species / metabolism
NADPH oxidase 2 biomarker colon cancer immunohistochemistry transfection

Journal

Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988

Informations de publication

Date de publication:
Jun 2023
Historique:
received: 25 03 2023
revised: 15 04 2023
accepted: 27 04 2023
medline: 31 5 2023
pubmed: 30 5 2023
entrez: 29 5 2023
Statut: ppublish

Résumé

Recent studies have reported that nicotinamide adenine dinucleotide phosphate oxidases (NOXs) are expressed in various cancers and play important roles in tumor progression. However, no studies have examined the expression and role of NOX2 in colon cancer. The aim of this study is to investigate the pathophysiological roles of NOX2 in colon cancer patients and cell lines. One-hundred and sixteen primary colon cancer samples of patients who underwent radical resection for locally advanced colon cancer were used for immunohistochemistry of NOX2 protein. The relationship between NOX2 expression and clinicopathological factors was assessed and the prognostic significance of NOX2 expression was evaluated in colon cancer patients. NOX2 siRNA transfection experiments were performed using two colon cancer cell lines (HCT116 and RKO) to analyze the impact of NOX2 expression on cellular physiological functions. The expression of NOX2 protein in noncancerous tissue was scarcely observed, and 45 samples (38.8%) showed positively stained NOX2 expression in cancer tissue. There were no clinicopathological factors significantly associated with NOX2 expression. The 5-year recurrence-free survival rate of the NOX2 positive group was significantly lower than that of the NOX2 negative group (61.1% vs. 79.3%, p=0.029). NOX2 depletion significantly inhibited cell proliferation with G1 arrest, and motility in the two cell lines. NOX2 expression level has a close association with the prognosis of colon cancer patients and physiological functions of colon cancer cells. NOX2 may be a useful prognostic biomarker for colon cancer patients.

Sections du résumé

BACKGROUND/AIM OBJECTIVE
Recent studies have reported that nicotinamide adenine dinucleotide phosphate oxidases (NOXs) are expressed in various cancers and play important roles in tumor progression. However, no studies have examined the expression and role of NOX2 in colon cancer. The aim of this study is to investigate the pathophysiological roles of NOX2 in colon cancer patients and cell lines.
PATIENTS AND METHODS METHODS
One-hundred and sixteen primary colon cancer samples of patients who underwent radical resection for locally advanced colon cancer were used for immunohistochemistry of NOX2 protein. The relationship between NOX2 expression and clinicopathological factors was assessed and the prognostic significance of NOX2 expression was evaluated in colon cancer patients. NOX2 siRNA transfection experiments were performed using two colon cancer cell lines (HCT116 and RKO) to analyze the impact of NOX2 expression on cellular physiological functions.
RESULTS RESULTS
The expression of NOX2 protein in noncancerous tissue was scarcely observed, and 45 samples (38.8%) showed positively stained NOX2 expression in cancer tissue. There were no clinicopathological factors significantly associated with NOX2 expression. The 5-year recurrence-free survival rate of the NOX2 positive group was significantly lower than that of the NOX2 negative group (61.1% vs. 79.3%, p=0.029). NOX2 depletion significantly inhibited cell proliferation with G1 arrest, and motility in the two cell lines.
CONCLUSION CONCLUSIONS
NOX2 expression level has a close association with the prognosis of colon cancer patients and physiological functions of colon cancer cells. NOX2 may be a useful prognostic biomarker for colon cancer patients.

Identifiants

DOI: 10.21873/anticanres.16427 PMID: 37247898
pubmed: 37247898
pii: 43/6/2601
doi: 10.21873/anticanres.16427
doi:

Substances chimiques

NADPH Oxidase 2 EC 1.6.3.-
NADPH Oxidases EC 1.6.3.-
Reactive Oxygen Species 0
CYBB protein, human EC 1.6.3.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2601-2608

Informations de copyright

Copyright © 2023 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Auteurs

Koichi Takiguchi (K)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Hiroki Shimizu (H)

Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan; hiro0810@koto.kpu-m.ac.jp.

Katsutoshi Shoda (K)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Kensuke Shiraishi (K)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Shinji Furuya (S)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Naohiro Hosomura (N)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Hidenori Akaike (H)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Yoshihiko Kawaguchi (Y)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Hidetake Amemiya (H)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Hiromichi Kawaida (H)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Kunio Mochizuki (K)

Department of Pathology, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Tetsuo Kondo (T)

Department of Pathology, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

Daisuke Ichikawa (D)

First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › The Expression and Role of NADPH Oxidase 2 in...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies gastro-intestinales › Maladies intestinales › Tumeurs de l'intestin › Tumeurs du côlon › The Expression and Role of NADPH Oxidase 2 in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › NADPH oxidase › NADPH Oxidase 2 › The Expression and Role of NADPH Oxidase 2 in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › NADPH oxidase › The Expression and Role of NADPH Oxidase 2 in...
questionsmedicales.fr › Diagnostic › Pronostic › The Expression and Role of NADPH Oxidase 2 in...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Espèces réactives de l'oxygène › The Expression and Role of NADPH Oxidase 2 in...