A previously uncharacterized Factor Associated with Metabolism and Energy (FAME/C14orf105/CCDC198/1700011H14Rik) is related to evolutionary adaptation, energy balance, and kidney physiology.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 05 2023
29 05 2023
Historique:
received:
24
09
2021
accepted:
11
05
2023
medline:
31
5
2023
pubmed:
30
5
2023
entrez:
29
5
2023
Statut:
epublish
Résumé
In this study we use comparative genomics to uncover a gene with uncharacterized function (1700011H14Rik/C14orf105/CCDC198), which we hereby name FAME (Factor Associated with Metabolism and Energy). We observe that FAME shows an unusually high evolutionary divergence in birds and mammals. Through the comparison of single nucleotide polymorphisms, we identify gene flow of FAME from Neandertals into modern humans. We conduct knockout experiments on animals and observe altered body weight and decreased energy expenditure in Fame knockout animals, corresponding to genome-wide association studies linking FAME with higher body mass index in humans. Gene expression and subcellular localization analyses reveal that FAME is a membrane-bound protein enriched in the kidneys. Although the gene knockout results in structurally normal kidneys, we detect higher albumin in urine and lowered ferritin in the blood. Through experimental validation, we confirm interactions between FAME and ferritin and show co-localization in vesicular and plasma membranes.
Identifiants
pubmed: 37248239
doi: 10.1038/s41467-023-38663-7
pii: 10.1038/s41467-023-38663-7
pmc: PMC10226981
doi:
Substances chimiques
Ferritins
9007-73-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3092Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2023. The Author(s).
Références
Cell. 2020 Feb 20;180(4):677-687.e16
pubmed: 32004458
Oncotarget. 2015 May 30;6(15):13718-30
pubmed: 25980435
Nature. 2001 Jan 11;409(6817):211-5
pubmed: 11196647
Nat Rev Genet. 2016 Feb;17(2):66
pubmed: 26659015
Nature. 2015 Oct 1;526(7571):68-74
pubmed: 26432245
Science. 2010 May 7;328(5979):710-722
pubmed: 20448178
Nature. 2012 Apr 04;484(7392):55-61
pubmed: 22481358
Nature. 2014 Aug 14;512(7513):194-7
pubmed: 25043035
Mol Biol Evol. 2014 Dec;31(12):3194-205
pubmed: 25169930
Invest Ophthalmol Vis Sci. 2006 Nov;47(11):4660-4
pubmed: 17065470
Sci Rep. 2017 Sep 8;7(1):11008
pubmed: 28887542
Neurosci Biobehav Rev. 1994 Fall;18(3):435-47
pubmed: 7984361
Nat Methods. 2011 Dec 28;9(1):57-63
pubmed: 22205519
Genesis. 2008 Sep;46(9):441-6
pubmed: 18781631
J Pediatr. 2019 Apr;207:198-204.e1
pubmed: 30630632
J Am Soc Nephrol. 2020 Oct;31(10):2341-2354
pubmed: 32651223
BMC Evol Biol. 2013 Jan 23;13:20
pubmed: 23342979
Bosn J Basic Med Sci. 2021 Feb 01;21(1):39-46
pubmed: 32156253
Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19619-19625
pubmed: 31506348
Nucleic Acids Res. 2018 Jan 4;46(D1):D1271-D1281
pubmed: 29106664
Science. 2014 Jan 3;343(6166):84-87
pubmed: 24336571
Genome Res. 2005 Mar;15(3):376-84
pubmed: 15710747
Sci Rep. 2017 Aug 15;7(1):8157
pubmed: 28811661
Mol Syst Biol. 2019 Feb 18;15(2):e8503
pubmed: 30777892
Antioxid Redox Signal. 2008 Jun;10(6):997-1030
pubmed: 18327971
Int J Obes Relat Metab Disord. 1995 Oct;19 Suppl 4:S22-30
pubmed: 8581091
Science. 2015 Nov 27;350(6264):1096-101
pubmed: 26472758
Biomed Pharmacother. 2017 Jul;91:1167-1177
pubmed: 28531945
Acta Pharmacol Sin. 2020 Aug;41(8):1005-1015
pubmed: 32203082
Nature. 2014 Jan 2;505(7481):43-9
pubmed: 24352235
Mol Ecol. 2008 Nov;17(21):4586-96
pubmed: 19140982
Sci Rep. 2018 Jan 10;8(1):334
pubmed: 29321513
J Exp Biol. 2016 Feb;219(Pt 3):300-1
pubmed: 26847558
Bioinformatics. 2019 Nov 1;35(22):4851-4853
pubmed: 31233103
J Biol Chem. 2006 Sep 29;281(39):29042-53
pubmed: 16885158
Mol Metab. 2019 Jun;24:64-79
pubmed: 30954544
Science. 2014 Dec 12;346(6215):1311-20
pubmed: 25504712
Front Physiol. 2016 Aug 03;7:337
pubmed: 27536250
Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):15132-15136
pubmed: 32546518
Nutr Neurosci. 2019 Mar;22(3):196-206
pubmed: 28784049
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Nature. 2005 Oct 20;437(7062):1153-7
pubmed: 16237444
Trends Biotechnol. 2010 Aug;28(8):398-406
pubmed: 20647113
Oncogene. 2018 Nov;37(44):5843-5857
pubmed: 29980787
Biol Cell. 2014 Aug;106(8):254-67
pubmed: 24867236
BMC Vet Res. 2015 Feb 07;11:22
pubmed: 25890299
Reproduction. 2016 Nov;152(5):R179-89
pubmed: 27486265
Nucleic Acids Res. 2021 Jan 8;49(D1):D884-D891
pubmed: 33137190
Brief Funct Genomic Proteomic. 2008 Jul;7(4):283-90
pubmed: 18641417
Science. 2018 May 18;360(6390):758-763
pubmed: 29622724
Nucleic Acids Res. 2018 Jul 2;46(W1):W242-W245
pubmed: 29762716
Trends Biochem Sci. 2016 Mar;41(3):274-286
pubmed: 26725301
Proc Natl Acad Sci U S A. 2017 Nov 28;114(48):E10256-E10257
pubmed: 29138326
Genesis. 2010 Feb;48(2):73-85
pubmed: 20095055
Science. 2014 Dec 12;346(6215):1320-31
pubmed: 25504713
Dev Cell. 2020 Apr 20;53(2):199-211.e6
pubmed: 32243782
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Ann N Y Acad Sci. 2016 Mar;1368(1):149-61
pubmed: 26890363
Sci Rep. 2019 Mar 18;9(1):4728
pubmed: 30894612
Onco Targets Ther. 2019 May 27;12:4081-4092
pubmed: 31239700
Nature. 2010 Oct 28;467(7319):1099-103
pubmed: 20981099
Nature. 2021 Jul;595(7865):120-124
pubmed: 34079125
J Physiol. 1949 Aug;109(1-2):1-9
pubmed: 15394301
Nat Protoc. 2018 Mar;13(3):530-550
pubmed: 29446774
Nucleic Acids Res. 2023 Jan 6;51(D1):D986-D993
pubmed: 36350644
Cell Syst. 2019 Apr 24;8(4):281-291.e9
pubmed: 30954476
Am J Physiol. 1992 Jun;262(6 Pt 2):R1025-32
pubmed: 1621856
Cell. 2010 Dec 23;143(7):1174-89
pubmed: 21183079
Anat Rec A Discov Mol Cell Evol Biol. 2004 Apr;277(2):344-54
pubmed: 15052662
Biopreserv Biobank. 2015 Oct;13(5):311-9
pubmed: 26484571
Science. 1970 Oct 2;170(3953):98-9
pubmed: 5452903
Methods Mol Biol. 2010;604:285-96
pubmed: 20013378
Science. 2017 Nov 3;358(6363):655-658
pubmed: 28982794
Nucleic Acids Res. 2004 Oct 12;32(18):5452-63
pubmed: 15479782
J Proteome Res. 2017 Dec 1;16(12):4330-4339
pubmed: 28960076
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Dev Cell. 2019 Nov 4;51(3):399-413.e7
pubmed: 31689386
Mol Biol Evol. 2011 Jul;28(7):2053-61
pubmed: 21245413
Nature. 2018 Oct;562(7727):367-372
pubmed: 30283141
Nat Commun. 2019 Jul 16;10(1):3131
pubmed: 31311925
Comp Biochem Physiol B Biochem Mol Biol. 2018 Oct;224:195-203
pubmed: 29128642
Antioxid Redox Signal. 2014 Aug 20;21(6):819-34
pubmed: 24738702
Curr Biol. 2016 Mar 21;26(6):827-33
pubmed: 26853362
Cell Metab. 2009 Aug;10(2):119-30
pubmed: 19656490
Science. 2019 Jun 07;364(6444):
pubmed: 31171666
Cell. 2004 Jul 23;118(2):203-16
pubmed: 15260990
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
pubmed: 15034147
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3882-7
pubmed: 20139300