The nuclear lamina couples mechanical forces to cell fate in the preimplantation embryo via actin organization.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 05 2023
29 05 2023
Historique:
received:
04
02
2023
accepted:
15
05
2023
medline:
31
5
2023
pubmed:
30
5
2023
entrez:
29
5
2023
Statut:
epublish
Résumé
During preimplantation development, contractile forces generated at the apical cortex segregate cells into inner and outer positions of the embryo, establishing the inner cell mass (ICM) and trophectoderm. To which extent these forces influence ICM-trophectoderm fate remains unresolved. Here, we found that the nuclear lamina is coupled to the cortex via an F-actin meshwork in mouse and human embryos. Actomyosin contractility increases during development, upregulating Lamin-A levels, but upon internalization cells lose their apical cortex and downregulate Lamin-A. Low Lamin-A shifts the localization of actin nucleators from nucleus to cytoplasm increasing cytoplasmic F-actin abundance. This results in stabilization of Amot, Yap phosphorylation and acquisition of ICM over trophectoderm fate. By contrast, in outer cells, Lamin-A levels increase with contractility. This prevents Yap phosphorylation enabling Cdx2 to specify the trophectoderm. Thus, forces transmitted to the nuclear lamina control actin organization to differentially regulate the factors specifying lineage identity.
Identifiants
pubmed: 37248263
doi: 10.1038/s41467-023-38770-5
pii: 10.1038/s41467-023-38770-5
pmc: PMC10226985
doi:
Substances chimiques
Actins
0
Adaptor Proteins, Signal Transducing
0
Cell Cycle Proteins
0
YAP-Signaling Proteins
0
Lamins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3101Subventions
Organisme : NICHD NIH HHS
ID : R01 HD102013
Pays : United States
Organisme : NICHD NIH HHS
ID : P50 HD068157
Pays : United States
Informations de copyright
© 2023. The Author(s).
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