New measures of anisotropy of cryo-EM maps.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
07 2023
07 2023
Historique:
received:
22
04
2022
accepted:
05
04
2023
medline:
12
7
2023
pubmed:
30
5
2023
entrez:
29
5
2023
Statut:
ppublish
Résumé
We propose two new measures of resolution anisotropy for cryogenic electron microscopy maps: Fourier shell occupancy (FSO), and the Bingham test (BT). FSO varies from 1 to 0, with 1 representing perfect isotropy, and lower values indicating increasing anisotropy. The threshold FSO = 0.5 occurs at Fourier shell correlation resolution. BT is a hypothesis test that complements the FSO to ensure the existence of anisotropy. FSO and BT allow visualization of resolution anisotropy. We illustrate their use with different experimental cryogenic electron microscopy maps.
Identifiants
pubmed: 37248387
doi: 10.1038/s41592-023-01874-3
pii: 10.1038/s41592-023-01874-3
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1021-1024Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM125769
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.
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