Primary Cultures of Spermatogonia and Testis Cells.

Culture Developmental biology Spermatogenesis Spermatogonia Spermatogonial stem cell Testis

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
medline: 1 6 2023
pubmed: 30 5 2023
entrez: 30 5 2023
Statut: ppublish

Résumé

Spermatogonial stem cells (SSCs) maintain adult spermatogenesis in mammals by undergoing self-renewal and differentiation into spermatozoa. In order to study the biology of SSCs as related to spermatogenesis, an in vitro, long-term expansion system of SSCs constitutes an ideal tool. In this chapter, we describe a robust culture system for mouse and rat SSCs in vitro. In the presence of GDNF, GFRα1, and bFGF, SSCs maintained on STO feeder layers with serum-free medium continuously proliferate for over 6 months. Complete spermatogenesis in infertile recipient mice can be attained following transplantation of the cultured mouse and rat SSCs. Using the in vitro SSC culture systems, elucidation of stem cell biology can be advanced that significantly advances our understanding of spermatogenesis and male fertility.

Identifiants

pubmed: 37249869
doi: 10.1007/978-1-0716-3139-3_7
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

127-143

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

de Rooij DG, Russell LD (2000) All you wanted to know about spermatozoon but were afraid to ask. J Androl 21:776–798
pubmed: 11105904
De Rooij DG, Griswold MD (2012) Questions about spermatogonia posed and answered since 2000. J Androl 33(6):1085–1095. https://doi.org/10.2164/jandrol.112.016832
doi: 10.2164/jandrol.112.016832 pubmed: 22879526
Brinster RL (2002) Germline stem cell transplantation and transgenesis. Science 296(5576):2174–2176. https://doi.org/10.1126/science.1071607
doi: 10.1126/science.1071607 pubmed: 12077400 pmcid: 4881847
Brinster RL (2007) Male germline stem cells: from mice to men. Science 316(5823):404–405. https://doi.org/10.1126/science.1137741
doi: 10.1126/science.1137741 pubmed: 17446391 pmcid: 4889115
Oatley JM, Avarbock MR, Telaranta AI, Fearon DT, Brinster RL (2006) Identifying genes important for spermatogonial stem cell self-renewal and survival. Proc Natl Acad Sci U S A 103(25):9524–9529. https://doi.org/10.1073/pnas.0603332103
doi: 10.1073/pnas.0603332103 pubmed: 16740658 pmcid: 1480440
Tegelenbosch RA, de Rooij DG (1993) A quantitative study of spermatogonial multiplication and stem cell renewal in the C3H/101 F1 hybrid mouse. Mutat Res 290(2):193–200. https://doi.org/10.1016/0027-5107(93)90159-d
doi: 10.1016/0027-5107(93)90159-d pubmed: 7694110
Kubota H, Brinster RL (2008) Culture of rodent spermatogonial stem cells, male germline stem cells of the postnatal animal. Methods Cell Biol 86:59–84. https://doi.org/10.1016/S0091-679X(08)00004-6
doi: 10.1016/S0091-679X(08)00004-6 pubmed: 18442644 pmcid: 4030707
Nagano M, Ryu BY, Brinster CJ, Avarbock MR, Brinster RL (2003) Maintenance of mouse male germ line stem cells in vitro. Biol Reprod 68(6):2207–2214. https://doi.org/10.1095/biolreprod.102.014050
doi: 10.1095/biolreprod.102.014050 pubmed: 12606373
Shinohara T, Avarbock MR, Brinster RL (1999) beta1- and alpha6-integrin are surface markers on mouse spermatogonial stem cells. Proc Natl Acad Sci U S A 96(10):5504–5509. https://doi.org/10.1073/pnas.96.10.5504
doi: 10.1073/pnas.96.10.5504 pubmed: 10318913 pmcid: 21889
Shinohara T, Orwig KE, Avarbock MR, Brinster RL (2000) Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells. Proc Natl Acad Sci U S A 97(15):8346–8351. https://doi.org/10.1073/pnas.97.15.8346
doi: 10.1073/pnas.97.15.8346 pubmed: 10900001 pmcid: 26950
Kubota H, Avarbock MR, Brinster RL (2004) Culture conditions and single growth factors affect fate determination of mouse spermatogonial stem cells. Biol Reprod 71(3):722–731. https://doi.org/10.1095/biolreprod.104.029207
doi: 10.1095/biolreprod.104.029207 pubmed: 15115718
Shinohara T, Avarbock MR, Brinster RL (2000) Functional analysis of spermatogonial stem cells in steel and cryptorchid infertile mouse models. Dev Biol 220(2):401–411. https://doi.org/10.1006/dbio.2000.9655
doi: 10.1006/dbio.2000.9655 pubmed: 10753526
Kubota H, Avarbock MR, Brinster RL (2003) Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells. Proc Natl Acad Sci U S A 100(11):6487–6492. https://doi.org/10.1073/pnas.0631767100
doi: 10.1073/pnas.0631767100 pubmed: 12738887 pmcid: 164473
Ryu BY, Kubota H, Avarbock MR, Brinster RL (2005) Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat. Proc Natl Acad Sci U S A 102(40):14302–14307. https://doi.org/10.1073/pnas.0506970102
doi: 10.1073/pnas.0506970102 pubmed: 16183739 pmcid: 1242328
Nagano M, Avarbock MR, Leonida EB, Brinster CJ, Brinster RL (1998) Culture of mouse spermatogonial stem cells. Tissue Cell 30(4):389–397. https://doi.org/10.1016/s0040-8166(98)80053-0
doi: 10.1016/s0040-8166(98)80053-0 pubmed: 9787472 pmcid: 5507671
Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S, Shinohara T (2003) Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol Reprod 69(2):612–616. https://doi.org/10.1095/biolreprod.103.017012
doi: 10.1095/biolreprod.103.017012 pubmed: 12700182
Wei X, Jia Y, Xue Y, Geng L, Wang M, Li L, Wang M, Zhang X, Wu X (2016) GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties. Sci Rep 6:36779. https://doi.org/10.1038/srep36779
doi: 10.1038/srep36779 pubmed: 27827452 pmcid: 5101510
Guo Y, Hai Y, Gong Y, Li Z, He Z (2014) Characterization, isolation, and culture of mouse and human spermatogonial stem cells. J Cell Physiol 229(4):407–413. https://doi.org/10.1002/jcp.24471
doi: 10.1002/jcp.24471 pubmed: 24114612
Li CH, Yan LZ, Ban WZ, Tu Q, Wu Y, Wang L, Bi R, Ji S, Ma YH, Nie WH, Lv LB, Yao YG, Zhao XD, Zheng P (2017) Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring. Cell Res 27(2):241–252. https://doi.org/10.1038/cr.2016.156
doi: 10.1038/cr.2016.156 pubmed: 28008926
Hamra FK, Chapman KM, Nguyen DM, Williams-Stephens AA, Hammer RE, Garbers DL (2005) Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture. Proc Natl Acad Sci U S A 102(48):17430–17435. https://doi.org/10.1073/pnas.0508780102
doi: 10.1073/pnas.0508780102 pubmed: 16293688 pmcid: 1283987
Kanatsu-Shinohara M, Miki H, Inoue K, Ogonuki N, Toyokuni S, Ogura A, Shinohara T (2005) Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions. Biol Reprod 72(4):985–991. https://doi.org/10.1095/biolreprod.104.036400
doi: 10.1095/biolreprod.104.036400 pubmed: 15601913
Kubota H, Avarbock MR, Brinster RL (2004) Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells. Proc Natl Acad Sci U S A 101(47):16489–16494. https://doi.org/10.1073/pnas.0407063101
doi: 10.1073/pnas.0407063101 pubmed: 15520394 pmcid: 534530
Kanatsu-Shinohara M, Inoue K, Lee J, Miki H, Ogonuki N, Toyokuni S, Ogura A, Shinohara T (2006) Anchorage-independent growth of mouse male germline stem cells in vitro. Biol Reprod 74(3):522–529. https://doi.org/10.1095/biolreprod.105.046441
doi: 10.1095/biolreprod.105.046441 pubmed: 16306420
Kanatsu-Shinohara M, Inoue K, Ogonuki N, Morimoto H, Ogura A, Shinohara T (2011) Serum- and feeder-free culture of mouse germline stem cells. Biol Reprod 84(1):97–105. https://doi.org/10.1095/biolreprod.110.086462
doi: 10.1095/biolreprod.110.086462 pubmed: 20844279
Kanatsu-Shinohara M, Ogonuki N, Matoba S, Morimoto H, Ogura A, Shinohara T (2014) Improved serum- and feeder-free culture of mouse germline stem cells. Biol Reprod 91(4):88. https://doi.org/10.1095/biolreprod.114.122317
doi: 10.1095/biolreprod.114.122317 pubmed: 25210127
Ishii K, Kanatsu-Shinohara M, Toyokuni S, Shinohara T (2012) FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAP2K1 activation. Development 139(10):1734–1743. https://doi.org/10.1242/dev.076539
doi: 10.1242/dev.076539 pubmed: 22491947
Aponte PM, Soda T, Teerds KJ, Mizrak SC, van de Kant HJ, de Rooij DG (2008) Propagation of bovine spermatogonial stem cells in vitro. Reproduction 136(5):543–557. https://doi.org/10.1530/REP-07-0419
doi: 10.1530/REP-07-0419 pubmed: 18663014
Sharma A, Shah SM, Saini N, Mehta P, Kumar BSB, Dua D, Singh MK, Singla SK, Palta P, Manik RS, Chauhan MS (2019) Optimization of serum-free culture conditions for propagation of Putative Buffalo (Bubalus bubalis) spermatogonial stem cells. Cell Reprogram 21(1):1–10. https://doi.org/10.1089/cell.2018.0018
doi: 10.1089/cell.2018.0018 pubmed: 30601028
Dolci S, Pellegrini M, Di Agostino S, Geremia R, Rossi P (2001) Signaling through extracellular signal-regulated kinase is required for spermatogonial proliferative response to stem cell factor. J Biol Chem 276(43):40225–40233. https://doi.org/10.1074/jbc.M105143200
doi: 10.1074/jbc.M105143200 pubmed: 11502745
Oatley JM, Oatley MJ, Avarbock MR, Tobias JW, Brinster RL (2009) Colony stimulating factor 1 is an extrinsic stimulator of mouse spermatogonial stem cell self-renewal. Development 136(7):1191–1199. https://doi.org/10.1242/dev.032243
doi: 10.1242/dev.032243 pubmed: 19270176 pmcid: 2685936
Huang YH, Chin CC, Ho HN, Chou CK, Shen CN, Kuo HC, Wu TJ, Wu YC, Hung YC, Chang CC, Ling TY (2009) Pluripotency of mouse spermatogonial stem cells maintained by IGF-1- dependent pathway. FASEB J 23(7):2076–2087. https://doi.org/10.1096/fj.08-121939
doi: 10.1096/fj.08-121939 pubmed: 19246485
Yang F, Whelan EC, Guan X, Deng B, Wang S, Sun J, Avarbock MR, Wu X, Brinster RL (2020) FGF9 promotes mouse spermatogonial stem cell proliferation mediated by p38 MAPK signalling. Cell Prolif 54. https://doi.org/10.1111/cpr.12933
Meng X, Lindahl M, Hyvonen ME, Parvinen M, de Rooij DG, Hess MW, Raatikainen-Ahokas A, Sainio K, Rauvala H, Lakso M, Pichel JG, Westphal H, Saarma M, Sariola H (2000) Regulation of cell fate decision of undifferentiated spermatogonia by GDNF. Science 287(5457):1489–1493. https://doi.org/10.1126/science.287.5457.1489
doi: 10.1126/science.287.5457.1489 pubmed: 10688798
Takashima S, Kanatsu-Shinohara M, Tanaka T, Morimoto H, Inoue K, Ogonuki N, Jijiwa M, Takahashi M, Ogura A, Shinohara T (2015) Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal. Stem Cell Reports 4(3):489–502. https://doi.org/10.1016/j.stemcr.2015.01.010
doi: 10.1016/j.stemcr.2015.01.010 pubmed: 25684228 pmcid: 4375941
Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL (2011) Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties. FASEB J 25(8):2604–2614. https://doi.org/10.1096/fj.10-175802
doi: 10.1096/fj.10-175802 pubmed: 21525489 pmcid: 3136339
Kanatsu-Shinohara M, Muneto T, Lee J, Takenaka M, Chuma S, Nakatsuji N, Horiuchi T, Shinohara T (2008) Long-term culture of male germline stem cells from hamster testes. Biol Reprod 78(4):611–617. https://doi.org/10.1095/biolreprod.107.065615
doi: 10.1095/biolreprod.107.065615 pubmed: 18094355
Kuijk EW, Colenbrander B, Roelen BA (2009) The effects of growth factors on in vitro-cultured porcine testicular cells. Reproduction 138(4):721–731. https://doi.org/10.1530/REP-09-0138
doi: 10.1530/REP-09-0138 pubmed: 19633132
Zhu H, Liu C, Li M, Sun J, Song W, Hua J (2013) Optimization of the conditions of isolation and culture of dairy goat male germline stem cells (mGSC). Anim Reprod Sci 137(1–2):45–52. https://doi.org/10.1016/j.anireprosci.2012.12.005
doi: 10.1016/j.anireprosci.2012.12.005 pubmed: 23290695
Kadam PH, Kala S, Agrawal H, Singh KP, Singh MK, Chauhan MS, Palta P, Singla SK, Manik RS (2013) Effects of glial cell line-derived neurotrophic factor, fibroblast growth factor 2 and epidermal growth factor on proliferation and the expression of some genes in buffalo (Bubalus bubalis) spermatogonial cells. Reprod Fertil Dev 25(8):1149–1157. https://doi.org/10.1071/RD12330
doi: 10.1071/RD12330 pubmed: 23171731
Zheng Y, Tian X, Zhang Y, Qin J, An J, Zeng W (2013) In vitro propagation of male germline stem cells from piglets. J Assist Reprod Genet 30(7):945–952. https://doi.org/10.1007/s10815-013-0031-0
doi: 10.1007/s10815-013-0031-0 pubmed: 23779100 pmcid: 3725231
Oatley MJ, Kaucher AV, Yang QE, Waqas MS, Oatley JM (2016) Conditions for long-term culture of cattle undifferentiated spermatogonia. Biol Reprod 95(1):14. https://doi.org/10.1095/biolreprod.116.139832
doi: 10.1095/biolreprod.116.139832 pubmed: 27251094
Sadri-Ardekani H, Mizrak SC, van Daalen SK, Korver CM, Roepers-Gajadien HL, Koruji M, Hovingh S, de Reijke TM, de la Rosette JJ, van der Veen F, de Rooij DG, Repping S, van Pelt AM (2009) Propagation of human spermatogonial stem cells in vitro. JAMA 302(19):2127–2134. https://doi.org/10.1001/jama.2009.1689
doi: 10.1001/jama.2009.1689 pubmed: 19920237
He Z, Kokkinaki M, Jiang J, Dobrinski I, Dym M (2010) Isolation, characterization, and culture of human spermatogonia. Biol Reprod 82(2):363–372. https://doi.org/10.1095/biolreprod.109.078550
doi: 10.1095/biolreprod.109.078550 pubmed: 19846602
Kokkinaki M, Djourabtchi A, Golestaneh N (2011) Long-term culture of human SSEA-4 positive Spermatogonial Stem Cells (SSCs). J Stem Cell Res Ther 2(2). https://doi.org/10.4172/2157-7633.S2-003
Sadri-Ardekani H, Akhondi MA, van der Veen F, Repping S, van Pelt AM (2011) In vitro propagation of human prepubertal spermatogonial stem cells. JAMA 305(23):2416–2418. https://doi.org/10.1001/jama.2011.791
doi: 10.1001/jama.2011.791 pubmed: 21673293
Hou JM, Niu MH, Liu LH, Zhu ZJ, Wang XB, Sun M, Yuan QQ, Yang S, Zeng WX, Liu Y, Li Z, He ZP (2015) Establishment and characterization of human germline stem cell line with unlimited proliferation potentials and no tumor formation. Sci Rep-Uk 5. doi:ARTN 16922. https://doi.org/10.1038/srep16922
Kossack N, Terwort N, Wistuba J, Ehmcke J, Schlatt S, Scholer H, Kliesch S, Gromoll J (2013) A combined approach facilitates the reliable detection of human spermatogonia in vitro. Hum Reprod 28(11):3012–3025. https://doi.org/10.1093/humrep/det336
doi: 10.1093/humrep/det336 pubmed: 24001715
Langenstroth D, Kossack N, Westernstroer B, Wistuba J, Behr R, Gromoll J, Schlatt S (2014) Separation of somatic and germ cells is required to establish primate spermatogonial cultures. Hum Reprod 29(9):2018–2031. https://doi.org/10.1093/humrep/deu157
doi: 10.1093/humrep/deu157 pubmed: 24963164
Zheng Y, Thomas A, Schmidt CM, Dann CT (2014) Quantitative detection of human spermatogonia for optimization of spermatogonial stem cell culture. Hum Reprod 29(11):2497–2511. https://doi.org/10.1093/humrep/deu232
doi: 10.1093/humrep/deu232 pubmed: 25267789 pmcid: 4191455
Medrano JV, Rombaut C, Simon C, Pellicer A, Goossens E (2016) Human spermatogonial stem cells display limited proliferation in vitro under mouse spermatogonial stem cell culture conditions. Fertil Steril 106(6):1539–1549. e1538. https://doi.org/10.1016/j.fertnstert.2016.07.1065
doi: 10.1016/j.fertnstert.2016.07.1065 pubmed: 27490045
Li L, Wang M, Wang M, Wu X, Geng L, Xue Y, Wei X, Jia Y, Wu X (2016) A long non-coding RNA interacts with Gfra1 and maintains survival of mouse spermatogonial stem cells. Cell Death Dis 7:e2140. https://doi.org/10.1038/cddis.2016.24
doi: 10.1038/cddis.2016.24 pubmed: 26962690 pmcid: 4823932
Wang M, Guo Y, Wang M, Zhou T, Xue Y, Du G, Wei X, Wang J, Qi L, Zhang H, Li L, Ye L, Guo X, Wu X (2017) The glial cell-derived neurotrophic factor (GDNF)-responsive phosphoprotein landscape identifies raptor phosphorylation required for Spermatogonial progenitor cell proliferation. Mol Cell Proteomics 16(6):982–997. https://doi.org/10.1074/mcp.M116.065797
doi: 10.1074/mcp.M116.065797 pubmed: 28408662 pmcid: 5461548
Wang M, Yu L, Wang S, Yang F, Wang M, Li L, Wu X (2020) LIN28A binds to meiotic gene transcripts and modulates their translation in male germ cells. J Cell Sci 133(12). https://doi.org/10.1242/jcs.242701
Du G, Wang X, Luo M, Xu W, Zhou T, Wang M, Yu L, Li L, Cai L, Wang PJ, Zhong Li J, Oatley JM, Wu X (2020) mRBPome capture identifies the RNA-binding protein TRIM71, an essential regulator of spermatogonial differentiation. Development 147(8). https://doi.org/10.1242/dev.184655
Kubota H, Avarbock MR, Schmidt JA, Brinster RL (2009) Spermatogonial stem cells derived from infertile Wv/Wv mice self-renew in vitro and generate progeny following transplantation. Biol Reprod 81(2):293–301. https://doi.org/10.1095/biolreprod.109.075960
doi: 10.1095/biolreprod.109.075960 pubmed: 19369648 pmcid: 2849820
Helsel AR, Oatley MJ, Oatley JM (2017) Glycolysis-optimized conditions enhance maintenance of regenerative integrity in mouse Spermatogonial stem cells during long-term culture. Stem Cell Reports 8(5):1430–1441. https://doi.org/10.1016/j.stemcr.2017.03.004
doi: 10.1016/j.stemcr.2017.03.004 pubmed: 28392219 pmcid: 5425612

Auteurs

Fan Yang (F)

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, China.

Jiachen Sun (J)

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, China.

Xin Wu (X)

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, China. xinwu@njmu.edu.cn.

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