THRIFTY-A High-throughput Single Muscle Fiber Typing Method Based on Immunofluorescence Detection.
Fiber type identification
Muscle
Muscle fiber type
Myosin heavy chain
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 May 2023
20 May 2023
Historique:
received:
08
01
2022
revised:
07
02
2023
accepted:
22
03
2023
medline:
30
5
2023
pubmed:
30
5
2023
entrez:
30
5
2023
Statut:
epublish
Résumé
Skeletal muscle consists of a mixture of fiber types with different functional and metabolic characteristics. The relative composition of these muscle fiber types has implications for muscle performance, whole-body metabolism, and health. However, analyses of muscle samples in a fiber type-dependent manner are very time consuming. Therefore, these are often neglected in favor of more time-efficient analyses on mixed muscle samples. Methods such as western blot and myosin heavy chain separation by SDS-PAGE have previously been utilized to fiber type-isolated muscle fibers. More recently, the introduction of the dot blot method significantly increased the speed of fiber typing. However, despite recent advancements, none of the current methodologies are feasible for large-scale investigations because of their time requirements. Here, we present the protocol for a new method, which we have named THRIFTY (high-
Identifiants
pubmed: 37251094
doi: 10.21769/BioProtoc.4678
pii: e4678
pmc: PMC10213079
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e4678Informations de copyright
Copyright © 2023 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors have no competing interests.
Références
Skelet Muscle. 2021 Nov 2;11(1):24
pubmed: 34727990
Diabetes. 2000 Jul;49(7):1092-5
pubmed: 10909963
J Clin Endocrinol Metab. 2022 Jun 16;107(7):e2729-e2737
pubmed: 35405014
Sci Rep. 2019 Apr 24;9(1):6473
pubmed: 31019216
J Appl Physiol (1985). 2021 Apr 1;130(4):1001-1014
pubmed: 33630680
J Gerontol A Biol Sci Med Sci. 2009 Mar;64(3):332-9
pubmed: 19196907
J Appl Physiol (1985). 2009 Apr;106(4):1412-8
pubmed: 19112158
J Physiol. 2015 Apr 15;593(8):2053-69
pubmed: 25640469
Am J Physiol Endocrinol Metab. 2002 Jun;282(6):E1191-6
pubmed: 12006347
J Physiol. 2022 Oct;600(20):4421-4438
pubmed: 36069036
Am J Physiol Endocrinol Metab. 2006 Jun;290(6):E1245-52
pubmed: 16434552
BMC Cardiovasc Disord. 2006 Jan 10;6:2
pubmed: 16403232