In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET.
Journal
Biophysical reports
ISSN: 2667-0747
Titre abrégé: Biophys Rep (N Y)
Pays: United States
ID NLM: 9918266001106676
Informations de publication
Date de publication:
14 Jun 2023
14 Jun 2023
Historique:
received:
26
01
2023
accepted:
27
04
2023
medline:
30
5
2023
pubmed:
30
5
2023
entrez:
30
5
2023
Statut:
epublish
Résumé
Förster resonance energy transfer (FRET) microscopy is used in numerous biophysical and biomedical applications to monitor inter- and intramolecular interactions and conformational changes in the 2-10 nm range. FRET is currently being extended to in vivo optical imaging, its main application being in quantifying drug-target engagement or drug release in animal models of cancer using organic dye or nanoparticle-labeled probes. Herein, we compared FRET quantification using intensity-based FRET (sensitized emission FRET analysis with the three-cube approach using an IVIS imager) and macroscopic fluorescence lifetime (MFLI) FRET using a custom system using a time-gated-intensified charge-coupled device, for small animal optical in vivo imaging. The analytical expressions and experimental protocols required to quantify the product
Identifiants
pubmed: 37251213
doi: 10.1016/j.bpr.2023.100110
pii: S2667-0747(23)00011-3
pmc: PMC10209493
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100110Subventions
Organisme : NCI NIH HHS
ID : R01 CA207725
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA237267
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA250636
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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