Saponin protects against cyclophosphamide-induced kidney and liver damage via antioxidant and anti-inflammatory actions.


Journal

Physiology international
ISSN: 2498-602X
Titre abrégé: Physiol Int
Pays: Hungary
ID NLM: 101696724

Informations de publication

Date de publication:
12 Jun 2023
Historique:
received: 22 01 2023
revised: 30 04 2023
accepted: 05 05 2023
medline: 14 6 2023
pubmed: 31 5 2023
entrez: 31 5 2023
Statut: epublish

Résumé

The liver and kidney are organs affected by chemotherapy drugs such as cyclophosphamide (CP). This study examined the protective effects of treatment with saponin (SP) against CP-induced nephrotoxicity and hepatotoxicity. 24 adult male mice were divided into four groups (N = 6): Control group, CP (15 mg kg-1), SP (2.5 mg kg-1) and CP + SP. After treatment, blood samples were collected for the determination of biochemical parameters. Liver and kidney samples were taken for histological analysis and assessment of oxidative stress and inflammatory markers. Cyclophosphamide decreased renal and liver functions and antioxidant enzymes, which significantly increased blood urea nitrogen and creatinine (BUN, Cr), liver enzyme levels, malondialdehyde, nuclear factor kappa β (NF-kB) and Interleukin 1 beta (IL-1B) concentrations. Moreover, histopathological findings of the CP group showed that there were acute tubular necrosis and glomerular atrophy in the renal tissues and lymphocyte infiltration in the liver samples. Treatment with saponin improved hepatic and renal functions, pathological changes and antioxidant capacity, and also decreased lipid peroxidation and inflammation. It seems that saponin could exert a hepato-nephroprotective effect against cyclophosphamide toxicity.

Sections du résumé

Background UNASSIGNED
The liver and kidney are organs affected by chemotherapy drugs such as cyclophosphamide (CP). This study examined the protective effects of treatment with saponin (SP) against CP-induced nephrotoxicity and hepatotoxicity.
Methods UNASSIGNED
24 adult male mice were divided into four groups (N = 6): Control group, CP (15 mg kg-1), SP (2.5 mg kg-1) and CP + SP. After treatment, blood samples were collected for the determination of biochemical parameters. Liver and kidney samples were taken for histological analysis and assessment of oxidative stress and inflammatory markers.
Results UNASSIGNED
Cyclophosphamide decreased renal and liver functions and antioxidant enzymes, which significantly increased blood urea nitrogen and creatinine (BUN, Cr), liver enzyme levels, malondialdehyde, nuclear factor kappa β (NF-kB) and Interleukin 1 beta (IL-1B) concentrations. Moreover, histopathological findings of the CP group showed that there were acute tubular necrosis and glomerular atrophy in the renal tissues and lymphocyte infiltration in the liver samples. Treatment with saponin improved hepatic and renal functions, pathological changes and antioxidant capacity, and also decreased lipid peroxidation and inflammation.
Conclusion UNASSIGNED
It seems that saponin could exert a hepato-nephroprotective effect against cyclophosphamide toxicity.

Identifiants

pubmed: 37256739
doi: 10.1556/2060.2023.00190
doi:

Substances chimiques

Antioxidants 0
Cyclophosphamide 8N3DW7272P
Anti-Inflammatory Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

108-120

Auteurs

Mohammad Ghasem Golmohammadi (MG)

1Department of Anatomical Sciences, School of Medicine, Research Laboratory for Embryology and Stem Cells, Ardabil University of Medical Sciences, Ardabil, Iran.

Shokofeh Banaei (S)

2Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

Mojgan Timar (M)

1Department of Anatomical Sciences, School of Medicine, Research Laboratory for Embryology and Stem Cells, Ardabil University of Medical Sciences, Ardabil, Iran.

Ali Abedi (A)

2Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

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Classifications MeSH