Multiple accurate and sensitive arrays for Capripoxvirus (CaPV) differentiation.

Animals Capripoxvirus / genetics Poxviridae Infections / diagnosis Goats / genetics Real-Time Polymerase Chain Reaction Nucleic Acid Amplification Techniques Sensitivity and Specificity
CRISPR/Cas12a Goatpox virus (GTPV) Lumpy skin disease virus (LSDV) Multiple-recombinase polymerase amplification (M-RPA) Real-time quantitative PCR (qPCR) Sheeppox virus (SPPV)

Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
01 Aug 2023
Historique:
received: 15 03 2023
revised: 16 05 2023
accepted: 17 05 2023
medline: 2 6 2023
pubmed: 1 6 2023
entrez: 31 5 2023
Statut: ppublish

Résumé

Capripoxvirus (CaPV) contains three viruses that have caused massive losses in the livestock and dairy industries. Accurate CaPV differentiation has far-reaching implications for effectively controlling outbreaks. However, it has a great challenge to distinguishing three viruses due to high homology of 97%. Here, we established a sensitive CRISPR/Cas12a array based on Multiple-recombinase polymerase amplification (M-RPA) for CaPV differentiation, which provided a more comprehensive and accurate differentiation mode targeting VARV B22R and RPO30 genes. By sensitive CRISPR/Cas12a and M-RPA, the actual detection limits of three viruses were as low as 50, 40 and 60 copies, respectively. Moreover, Lateral flow dipstick (LFD) array based on CRISPR/Cas12a achieved portable and intuitive detection, making it suitable for point-of-care testing. Therefore, CRISPR/Cas12a array and LFD array paved the way for CaPV differentiation in practice. Additionally, we constructed a real-time quantitative PCR (qPCR) array to fill the qPCR technical gap in differentiation and to facilitate the quarantine departments.

Identifiants

DOI: 10.1016/j.aca.2023.341391 PMID: 37257965
pubmed: 37257965
pii: S0003-2670(23)00612-8
doi: 10.1016/j.aca.2023.341391
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

341391

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Gaihua Cao (G)

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.

Yifan Xiong (Y)

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.

Meimei Shi (M)

State Key Laboratory of Cattle Diseases Detection (Chongqing) of Customs, Diagnosis and Testing Laboratory of Lumpy Skin Disease, Chongqing Customs Technology Center, Chongqing, 400020, PR China.

Yue Qiu (Y)

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.

Yu Wang (Y)

State Key Laboratory of Cattle Diseases Detection (Chongqing) of Customs, Diagnosis and Testing Laboratory of Lumpy Skin Disease, Chongqing Customs Technology Center, Chongqing, 400020, PR China.

Fuping Nie (F)

State Key Laboratory of Cattle Diseases Detection (Chongqing) of Customs, Diagnosis and Testing Laboratory of Lumpy Skin Disease, Chongqing Customs Technology Center, Chongqing, 400020, PR China. Electronic address: nie1626@163.com.

Danqun Huo (D)

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China. Electronic address: huodq@cqu.edu.cn.

Changjun Hou (C)

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, PR China. Electronic address: houcj@cqu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Multiple accurate and sensitive arrays for...
questionsmedicales.fr › Virus › Virus à ADN › Poxviridae › Chordopoxvirinae › Capripoxvirus › Multiple accurate and sensitive arrays for...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus à ADN › Infections à Poxviridae › Multiple accurate and sensitive arrays for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Capra › Multiple accurate and sensitive arrays for...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Multiple accurate and sensitive arrays for...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Multiple accurate and sensitive arrays for...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Multiple accurate and sensitive arrays for...