A Spectrofluorometric Method for Real-Time Graft Assessment and Patient Monitoring.
biomarkers
biomedical engineering
biosensors
liver transplantation
translational research
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
03
05
2023
received:
09
03
2023
medline:
17
8
2023
pubmed:
2
6
2023
entrez:
2
6
2023
Statut:
ppublish
Résumé
Biomarkers are powerful clinical diagnostics and predictors of patient outcome. However, robust measurements often require time and expensive laboratory equipment, which is insufficient to track rapid changes and limits direct use in the operating room. Here, this study presents a portable spectrophotometric device for continuous real-time measurements of fluorescent and non-fluorescent biomarkers at the point of care. This study measures the mitochondrial damage biomarker flavin mononucleotide (FMN) in 26 extended criteria human liver grafts undergoing hypothermic oxygenated perfusion to guide clinical graft assessment. Real-time data identified seven organs unsuitable for transplant that are discarded. The remaining grafts are transplanted and FMN values correlated with post-transplant indicators of liver function and patient recovery. Further, this study shows how this device can be used to monitor dialysis patients by measuring creatinine in real-time. Our approach provides a simple method to monitor biomarkers directly within biological fluids to improve organ assessment, patient care, and biomarker discovery.
Identifiants
pubmed: 37265001
doi: 10.1002/advs.202301537
pmc: PMC10427358
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2301537Subventions
Organisme : Eidgenössische Technische Hochschule Zürich
ID : ETH-24 21-1
Organisme : Wyss Zurich Translational Center
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Liver Transpl. 2005 Jul;11(7):814-825
pubmed: 15973726
Am J Transplant. 2006 Apr;6(4):783-90
pubmed: 16539636
Biomedicines. 2021 Feb 07;9(2):
pubmed: 33562406
Proteomics Clin Appl. 2015 Apr;9(3-4):289-94
pubmed: 25656266
N Engl J Med. 1987 Oct 8;317(15):909-16
pubmed: 2442609
Cell Rep. 2018 May 29;23(9):2617-2628
pubmed: 29847793
Nat Biotechnol. 2022 Nov;40(11):1610-1616
pubmed: 35641829
Ann Surg. 2017 Nov;266(5):772-778
pubmed: 28742700
Liver Transpl. 2020 Feb;26(2):276-282
pubmed: 31765044
Transplantation. 2001 May 27;71(10):1424-8
pubmed: 11391230
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Apr 15;1113:14-19
pubmed: 30877982
Nature. 2011 Jan 13;469(7329):156-7
pubmed: 21228852
Nature. 2014 Nov 20;515(7527):431-435
pubmed: 25383517
Hemodial Int. 2007 Oct;11 Suppl 3:S7-12
pubmed: 17897111
Antioxid Redox Signal. 2019 Sep 20;31(9):608-622
pubmed: 31037949
J Invest Surg. 2014 Dec;27(6):366-79
pubmed: 25058854
Cytometry A. 2019 Jan;95(1):34-46
pubmed: 30211978
Int Urol Nephrol. 2023 Jan;55(1):201-209
pubmed: 35877031
Kidney Int. 2006 Mar;69(6):1073-80
pubmed: 16528257
Br J Med Surg Urol. 2012 Jul 1;5(4):162-168
pubmed: 22712027
Curr Protoc Pharmacol. 2017 Mar 17;76:9.23.1-9.23.17
pubmed: 28306150
Nat Biotechnol. 2020 Feb;38(2):189-198
pubmed: 31932726
Nat Rev Dis Primers. 2018 Sep 13;4(1):23
pubmed: 30213943
Bioanalysis. 2010 Feb;2(2):311-41
pubmed: 20352077
Liver Transpl. 2012 Nov;18(11):1290-301
pubmed: 22847917
Annu Rev Biochem. 2013;82:551-75
pubmed: 23527692
J Hepatol. 2014 Apr;60(4):765-72
pubmed: 24295869
Int J Surg. 2020 Oct;82S:44-51
pubmed: 32353556
J Hepatol. 2014 Nov;61(5):1186-7
pubmed: 25051295
J Clin Med. 2020 Mar 20;9(3):
pubmed: 32244972
Ann Surg. 2019 Nov;270(5):783-790
pubmed: 31592808
Adv Sci (Weinh). 2023 Aug;10(23):e2301537
pubmed: 37265001
Curr Transplant Rep. 2018;5(1):72-81
pubmed: 29564205
Curr Transplant Rep. 2020;7(3):194-204
pubmed: 32837828
Arch Pathol Lab Med. 2005 Mar;129(3):297-304
pubmed: 15737021
Curr Opin Nephrol Hypertens. 1995 Nov;4(6):505-9; discussion 503-4
pubmed: 8591059
Sci Rep. 2018 Feb 9;8(1):2724
pubmed: 29426833
Am J Transplant. 2018 Aug;18(8):2005-2020
pubmed: 29419931
Int J Mol Sci. 2021 Mar 22;22(6):
pubmed: 33810024
Eur Urol. 2008 Mar;53(3):468-77
pubmed: 17997011
N Am J Med Sci. 2010 Apr;2(4):170-3
pubmed: 22624135
EBioMedicine. 2020 Oct;60:103014
pubmed: 32979838
Neurochem Res. 2013 May;38(5):895-905
pubmed: 23462880
Neurotherapeutics. 2011 Jul;8(3):349-60
pubmed: 21671123
J Hepatol. 2011 Oct;55(4):808-13
pubmed: 21338639
J Hepatol. 2019 Oct;71(4):719-730
pubmed: 31201834
N Engl J Med. 2018 May 17;378(20):1920-1929
pubmed: 29768153
Ann Surg. 2011 Apr;253(4):817-25
pubmed: 21475025
Curr Opin HIV AIDS. 2010 Nov;5(6):463-6
pubmed: 20978388
Am J Transplant. 2012 Oct;12(10):2789-96
pubmed: 22823098
Nephron. 1990;55(2):121-8
pubmed: 2362625
Transpl Int. 2015 Apr;28(4):492-501
pubmed: 25601020
Anal Chim Acta. 2020 Oct 23;1135:116-122
pubmed: 33070847
Sensors (Basel). 2018 Jun 22;18(7):
pubmed: 29932161