An exome-wide study of renal operational tolerance.

Homer2 IQCH LCN2 NGAL exome sequencing operational tolerance primary cilium renal transplantation

Journal

Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047

Informations de publication

Date de publication:
2022
Historique:
received: 23 06 2022
accepted: 31 10 2022
medline: 2 6 2023
pubmed: 2 6 2023
entrez: 2 6 2023
Statut: epublish

Résumé

Renal operational tolerance is a rare and beneficial state of prolonged renal allograft function in the absence of immunosuppression. The underlying mechanisms are unknown. We hypothesized that tolerance might be driven by inherited protein coding genetic variants with large effect, at least in some patients. We set up a European survey of over 218,000 renal transplant recipients and collected DNAs from 40 transplant recipients who maintained good allograft function without immunosuppression for at least 1 year. We performed an exome-wide association study comparing the distribution of moderate to high impact variants in 36 tolerant patients, selected for genetic homogeneity using principal component analysis, and 192 controls, using an optimal sequence-kernel association test adjusted for small samples. We identified rare variants of Rare protein coding variants are associated with operational tolerance in a sizable portion of patients. Our findings have important implications for a better understanding of immune tolerance in transplantation and other fields of medicine.ClinicalTrials.gov, identifier: NCT05124444.

Sections du résumé

Background UNASSIGNED
Renal operational tolerance is a rare and beneficial state of prolonged renal allograft function in the absence of immunosuppression. The underlying mechanisms are unknown. We hypothesized that tolerance might be driven by inherited protein coding genetic variants with large effect, at least in some patients.
Methods UNASSIGNED
We set up a European survey of over 218,000 renal transplant recipients and collected DNAs from 40 transplant recipients who maintained good allograft function without immunosuppression for at least 1 year. We performed an exome-wide association study comparing the distribution of moderate to high impact variants in 36 tolerant patients, selected for genetic homogeneity using principal component analysis, and 192 controls, using an optimal sequence-kernel association test adjusted for small samples.
Results UNASSIGNED
We identified rare variants of
Conclusion UNASSIGNED
Rare protein coding variants are associated with operational tolerance in a sizable portion of patients. Our findings have important implications for a better understanding of immune tolerance in transplantation and other fields of medicine.ClinicalTrials.gov, identifier: NCT05124444.

Identifiants

pubmed: 37265662
doi: 10.3389/fmed.2022.976248
pmc: PMC10230038
doi:

Banques de données

ClinicalTrials.gov
['NCT05124444']

Types de publication

Journal Article

Langues

eng

Pagination

976248

Investigateurs

Maria Aguilar Rodríguez (M)
Friederike Bachmann (F)
Rajendra Bahadur Shahi (RB)
Frederike Bemelman (F)
Luboslav Bena (L)
Luigi Biancone (L)
Laura Braun (L)
Klemens Budde (K)
Alejandro Camargo-Salamanca (A)
Katia Clemente (K)
Hulya Colak (H)
Adrian Covic (A)
Jacques Degreve (J)
Philippe Gatault (P)
François Glowacki (F)
Karine Hadaya (K)
Marc Hazzan (M)
Bénédicte Janbon (B)
Christophe Legendre (C)
Umberto Maggiore (U)
Marius Miglinas (M)
Anja Mühlfeld (A)
Maarten Naesens (M)
Christian Noël (C)
Rainer Oberbauer (R)
Evangeline Pillebout (E)
Gian Benedetto Piredda (GB)
Francesco Pisani (F)
Ana Ramírez Puga (AR)
Tomas Reischig (T)
Francisco González-Roncero (F)
Søren Schwartz Sørensen (SS)
Daniel Seron Micas (DS)
Nurhan Seyahi (N)
Dimitrie Siriopol (D)
Goce Spasovski (G)
Jean-François Subra (JF)
Erik Teugels (E)
Serhan Tuǧlular (S)
Sonia Van Dooren (S)
Catheline Vilain (C)
Florence Villemain (F)
Xavier Warling (X)
Bruno Watschinger (B)
Laurent Weekers (L)

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Copyright © 2023 Massart, Danger, Olsen, Emond, Viklicky, Jacquemin, Soblet, Duerinckx, Croes, Perazzolo, Hruba, Daneels, Caljon, Sever, Pascual, Miglinas, the Renal Tolerance Investigators, Pirson, Ghisdal, Smits, Giral, Abramowicz, Abramowicz and Brouard.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Annick Massart (A)

Human Genetics Unit, Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), Brussels, Belgium.
Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.
Department of Nephrology, Antwerp University Hospital and Laboratory of Experimental Medicine, University of Antwerp, Antwerp, Belgium.

Richard Danger (R)

CHU Nantes, Nantes Université, INSERM, Center for Research in Transplantation and Translational Immunology, CR2TI, UMR 1064, ITUN, Nantes, France.

Catharina Olsen (C)

Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.
Brussels Interuniversity Genomics High Throughput Core (BRIGHTcore), VUB-ULB, Brussels, Belgium.
Center for Medical Genetics, Reproduction and Genetics, Reproduction Genetics and Regenerative Medicine, Vrije Universiteit Brussel, UZ Brussel, Brussels, Belgium.

Mary J Emond (MJ)

Department of Biostatistics, University of Washington, Seattle, WA, United States.

Ondrej Viklicky (O)

Transplant Laboratory, Institute for Clinical and Experimental Medicine, Prague, Czechia.

Valérie Jacquemin (V)

Human Genetics Unit, Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), Brussels, Belgium.
Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.

Julie Soblet (J)

Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.
Department of Genetics, Hôpital Erasme, ULB Center of Human Genetics, Université Libre de Bruxelles, Brussels, Belgium.
Department of Genetics, Hôpital Universitaire des Enfants Reine Fabiola, ULB Center of Human Genetics, Université Libre de Bruxelles, Brussels, Belgium.

Sarah Duerinckx (S)

Human Genetics Unit, Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), Brussels, Belgium.
Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.

Didier Croes (D)

Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.
Brussels Interuniversity Genomics High Throughput Core (BRIGHTcore), VUB-ULB, Brussels, Belgium.
Center for Medical Genetics, Reproduction and Genetics, Reproduction Genetics and Regenerative Medicine, Vrije Universiteit Brussel, UZ Brussel, Brussels, Belgium.
Center for Human Genetics, Clinique Universitaires Saint Luc, Brussels, Belgium.

Camille Perazzolo (C)

Human Genetics Unit, Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), Brussels, Belgium.

Petra Hruba (P)

Transplant Laboratory, Institute for Clinical and Experimental Medicine, Prague, Czechia.

Dorien Daneels (D)

Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.
Brussels Interuniversity Genomics High Throughput Core (BRIGHTcore), VUB-ULB, Brussels, Belgium.
Center for Medical Genetics, Reproduction and Genetics, Reproduction Genetics and Regenerative Medicine, Vrije Universiteit Brussel, UZ Brussel, Brussels, Belgium.

Ben Caljon (B)

Brussels Interuniversity Genomics High Throughput Core (BRIGHTcore), VUB-ULB, Brussels, Belgium.
Center for Medical Genetics, Reproduction and Genetics, Reproduction Genetics and Regenerative Medicine, Vrije Universiteit Brussel, UZ Brussel, Brussels, Belgium.

Mehmet Sukru Sever (MS)

Istanbul Tip Fakültesi, Istanbul School of Medicine, Internal Medicine, Nephrology, Istanbul, Türkiye.

Julio Pascual (J)

Department of Nephrology, Hospital del Mar, Institute Mar for Medical Research, Barcelona, Spain.
Department of Nephrology, Hospital Universitario 12 de Octubre, Madrid, Spain.

Marius Miglinas (M)

Nephrology Center, Santaros Klinikos, Medical Faculty, Vilnius University, Vilnius, Lithuania.

Isabelle Pirson (I)

Human Genetics Unit, Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), Brussels, Belgium.

Lidia Ghisdal (L)

Department of Nephrology, Hospital Centre EpiCURA, Baudour, Belgium.

Guillaume Smits (G)

Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.
Department of Genetics, Hôpital Erasme, ULB Center of Human Genetics, Université Libre de Bruxelles, Brussels, Belgium.

Magali Giral (M)

CHU Nantes, Nantes Université, INSERM, Center for Research in Transplantation and Translational Immunology, CR2TI, UMR 1064, ITUN, Nantes, France.
CHU Nantes, Centre d'Investigation Clinique en Biothérapie, Centre de Ressources Biologiques (CRB), Nantes, France.
LabEx IGO "Immunotherapy, Graft, Oncology", Nantes, France.

Daniel Abramowicz (D)

Department of Nephrology, Antwerp University Hospital and Laboratory of Experimental Medicine, University of Antwerp, Antwerp, Belgium.

Marc Abramowicz (M)

Human Genetics Unit, Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), Brussels, Belgium.
Interuniversity Institute of Bioinformatics in Brussels (IB2), Université Libre de Bruxelles - Vrije Universiteit Brussel (ULB-VUB), Brussels, Belgium.
Department of Genetic Medicine and Development, Faculty of Medicine, Université de Geneve, Geneva, Switzerland.

Sophie Brouard (S)

CHU Nantes, Nantes Université, INSERM, Center for Research in Transplantation and Translational Immunology, CR2TI, UMR 1064, ITUN, Nantes, France.
CHU Nantes, Centre d'Investigation Clinique en Biothérapie, Centre de Ressources Biologiques (CRB), Nantes, France.
LabEx IGO "Immunotherapy, Graft, Oncology", Nantes, France.

Classifications MeSH