Characterization of the pheromone receptors in Mythimna loreyi reveals the differentiation of sex pheromone recognition in Mythimna species.
Mythimna loreyi
Xenopus oocyte
antennal transcriptome
pheromone receptor
sex pheromone
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
03 Jun 2023
03 Jun 2023
Historique:
revised:
31
03
2023
received:
28
12
2022
accepted:
17
04
2023
medline:
3
6
2023
pubmed:
3
6
2023
entrez:
3
6
2023
Statut:
aheadofprint
Résumé
Pheromone receptors (PRs) are key proteins in the molecular mechanism of pheromone recognition, and exploring the functional differentiation of PRs between closely related species helps to understand the evolution of moth mating systems. Pheromone components of the agricultural pest Mythimna loreyi have turned into (Z)-9-tetradecen-1-yl acetate (Z9-14:OAc), (Z)-7-dodecen-1-yl acetate (Z7-12:OAc), and (Z)-11-hexadecen-1-yl acetate, while the composition differs from that of M. separata in the genus Mythimna. To understand the molecular mechanism of pheromone recognition, we sequenced and analyzed antennal transcriptomes to identify 62 odorant receptor (OR) genes. The expression levels of all putative ORs were analyzed using differentially expressed gene analysis. Six candidate PRs were quantified and functionally characterized in the Xenopus oocytes system. MlorPR6 and MlorPR3 were determined to be the receptors of major and minor components Z9-14:OAc and Z7-12:OAc. MlorPR1 and female antennae (FA)-biased MlorPR5 both possessed the ability to detect pheromones of sympatric species, including (Z,E)-9,12-tetradecadien-1-ol, (Z)-9-tetradecen-1-ol, and (Z)-9-tetradecenal. Based on the comparison of PR functions between M. loreyi and M. separata, we analyzed the differentiation of pheromone recognition mechanisms during the evolution of the mating systems of 2 Mythimna species.
Identifiants
pubmed: 37269179
doi: 10.1111/1744-7917.13215
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 31725023
Organisme : National Natural Science Foundation of China
ID : 32072509
Organisme : National Natural Science Foundation of China
ID : 32130089
Organisme : Shenzhen Science and Technology Program
ID : KQTD20180411143628272
Organisme : Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District
ID : PT202101-02
Organisme : Agricultural Science and Technology Innovation Program
Informations de copyright
© 2023 Institute of Zoology, Chinese Academy of Sciences.
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