Elucidating the mechanisms of additive effects at high concentrations on hydrophobic interaction chromatography.
Cavity theory
Dehydration mechanism
Hydrophobic interaction chromatography
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
02 Aug 2023
02 Aug 2023
Historique:
received:
04
03
2023
revised:
17
05
2023
accepted:
19
05
2023
medline:
6
6
2023
pubmed:
4
6
2023
entrez:
3
6
2023
Statut:
ppublish
Résumé
Hydrophobic interaction chromatography (HIC) is a commonly used chromatography technique for purifying proteins. It utilizes salting-out salts to facilitate the binding of native proteins to weakly hydrophobic ligands. There have been three proposed mechanisms for the promoting effects of salting-out salts, which include the dehydration of proteins by salts, cavity theory, and salt exclusion. To evaluate the above three mechanisms, an HIC study was conducted on Phenyl Sepharose using four different additives. These additives included a salting-out salt (NH
Identifiants
pubmed: 37269642
pii: S0021-9673(23)00317-5
doi: 10.1016/j.chroma.2023.464091
pii:
doi:
Substances chimiques
Salts
0
Proteins
0
Sodium Chloride
451W47IQ8X
Polyethylene Glycols
3WJQ0SDW1A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
464091Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest This work was supported by Kyokuto Pharmaceutical Industrial Co., Ltd. Y.T., Y.K. and T.A. (Teruo Akuta) are employees of the for-profit company Kyokuto Pharmaceuticals. T.A. (Tsutomu Arakawa) used to belong to the for-profit company Alliance Protein Laboratories but currently has no conflict of interest.