In vitro infection of primary chicken lymphocytes with Marek's disease virus.
Cell Biology
Cell isolation
Cell-based Assays
Microbiology
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
03 Jun 2023
03 Jun 2023
Historique:
received:
24
03
2023
revised:
25
04
2023
accepted:
10
05
2023
medline:
4
6
2023
pubmed:
4
6
2023
entrez:
4
6
2023
Statut:
aheadofprint
Résumé
Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that infects immune cells and causes a deadly lymphoproliferative disease in chickens. Cytokines and monoclonal antibodies promote the survival of chicken lymphocytes in vitro. Here, we describe protocols for the isolation, maintenance, and efficient MDV infection of primary chicken lymphocytes and lymphocyte cell lines. This facilitates the investigation of key aspects of the MDV life cycle in the primary target cells of viral replication, latency, genome integration, and reactivation. For complete details on the use and execution of this protocol, please refer to Schermuly et al.,
Identifiants
pubmed: 37270781
pii: S2666-1667(23)00310-6
doi: 10.1016/j.xpro.2023.102343
pmc: PMC10276148
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102343Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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