Measuring the acidification of the phagosomal lumen in live C. elegans embryos.
Cell Biology
Developmental Biology
Microscopy
Model Organisms
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
03 Jun 2023
03 Jun 2023
Historique:
received:
16
12
2022
revised:
10
04
2023
accepted:
08
05
2023
medline:
4
6
2023
pubmed:
4
6
2023
entrez:
4
6
2023
Statut:
aheadofprint
Résumé
In metazoans, the acidification of the phagosomal lumen is essential for the efficient degradation of cargoes. Here, we present a protocol for measuring the rate of acidification inside phagosomal lumen containing apoptotic cells in living C. elegans embryos. We describe steps for generating a worm population, selecting embryos, and mounting embryos on agar pads. We then detail live imaging of embryos and data analysis. This protocol is applicable to any organism in which real-time fluorescence imaging can be performed. For complete details on the use and execution of this protocol, please refer to Pena-Ramos et al. (2022).
Identifiants
pubmed: 37270784
pii: S2666-1667(23)00299-X
doi: 10.1016/j.xpro.2023.102332
pmc: PMC10276153
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102332Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM067848
Pays : United States
Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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