Dynamic Glycoprotein Hyposialylation Promotes Chemotherapy Evasion and Metastatic Seeding of Quiescent Circulating Tumor Cell Clusters in Breast Cancer.


Journal

Cancer discovery
ISSN: 2159-8290
Titre abrégé: Cancer Discov
Pays: United States
ID NLM: 101561693

Informations de publication

Date de publication:
06 09 2023
Historique:
received: 03 06 2022
revised: 14 04 2023
accepted: 30 05 2023
medline: 7 9 2023
pubmed: 5 6 2023
entrez: 5 6 2023
Statut: ppublish

Résumé

Most circulating tumor cells (CTC) are detected as single cells, whereas a small proportion of CTCs in multicellular clusters with stemness properties possess 20- to 100-times higher metastatic propensity than the single cells. Here we report that CTC dynamics in both singles and clusters in response to therapies predict overall survival for breast cancer. Chemotherapy-evasive CTC clusters are relatively quiescent with a specific loss of ST6GAL1-catalyzed α2,6-sialylation in glycoproteins. Dynamic hyposialylation in CTCs or deficiency of ST6GAL1 promotes cluster formation for metastatic seeding and enables cellular quiescence to evade paclitaxel treatment in breast cancer. Glycoproteomic analysis reveals newly identified protein substrates of ST6GAL1, such as adhesion or stemness markers PODXL, ICAM1, ECE1, ALCAM1, CD97, and CD44, contributing to CTC clustering (aggregation) and metastatic seeding. As a proof of concept, neutralizing antibodies against one newly identified contributor, PODXL, inhibit CTC cluster formation and lung metastasis associated with paclitaxel treatment for triple-negative breast cancer. This study discovers that dynamic loss of terminal sialylation in glycoproteins of CTC clusters contributes to the fate of cellular dormancy, advantageous evasion to chemotherapy, and enhanced metastatic seeding. It identifies PODXL as a glycoprotein substrate of ST6GAL1 and a candidate target to counter chemoevasion-associated metastasis of quiescent tumor cells. This article is featured in Selected Articles from This Issue, p. 1949.

Identifiants

pubmed: 37272843
pii: 727129
doi: 10.1158/2159-8290.CD-22-0644
pmc: PMC10481132
mid: NIHMS1907326
doi:

Substances chimiques

Paclitaxel P88XT4IS4D
Glycoproteins 0
Biomarkers, Tumor 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2050-2071

Subventions

Organisme : NCI NIH HHS
ID : R01 CA236904
Pays : United States
Organisme : NCI NIH HHS
ID : UG3 CA256967
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA245699
Pays : United States
Organisme : NIH HHS
ID : S10 OD018530
Pays : United States
Organisme : NIDCR NIH HHS
ID : R21 DE025825
Pays : United States
Organisme : NCI NIH HHS
ID : UH3 CA256967
Pays : United States
Organisme : NIGMS NIH HHS
ID : R24 GM137782
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM115234
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

©2023 The Authors; Published by the American Association for Cancer Research.

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Auteurs

Nurmaa K Dashzeveg (NK)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Yuzhi Jia (Y)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Youbin Zhang (Y)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Lorenzo Gerratana (L)

Department of Medicinal Oncology, Centro di Riferimento Oncologico di Aviano (CRO), IRCCS, Aviano, Italy.

Priyam Patel (P)

Quantitative Data Science Core, Center for Genetic Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Asif Shajahan (A)

Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia.

Tsogbadrakh Dandar (T)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Erika K Ramos (EK)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Hannah F Almubarak (HF)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Valery Adorno-Cruz (V)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Rokana Taftaf (R)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Emma J Schuster (EJ)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

David Scholten (D)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Michael T Sokolowski (MT)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Carolina Reduzzi (C)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Division of Hematology-Oncology, Department of Medicine, Weill Cornell Medicine, New York, New York.

Lamiaa El-Shennawy (L)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Andrew D Hoffmann (AD)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Maroua Manai (M)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Qiang Zhang (Q)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Paolo D'Amico (P)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Parastoo Azadi (P)

Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia.

Karen J Colley (KJ)

Department of Biochemistry and Molecular Genetics, University of Illinois Chicago, Chicago, Illinois.

Leonidas C Platanias (LC)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Ami N Shah (AN)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

William J Gradishar (WJ)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Massimo Cristofanilli (M)

Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Division of Hematology-Oncology, Department of Medicine, Weill Cornell Medicine, New York, New York.
Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

William A Muller (WA)

Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio.

Brian A Cobb (BA)

Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Huiping Liu (H)

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Department of Medicine, Division of Hematology and Oncology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

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