Quantification of Surface Tension Effects and Nucleation-and-Growth Rates during Self-Assembly of Biological Condensates.
biological condensates
liquid-liquid phase separation
nucleation-and-growth
protein aggregation
surface tension
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
12
05
2023
received:
07
03
2023
medline:
17
8
2023
pubmed:
6
6
2023
entrez:
6
6
2023
Statut:
ppublish
Résumé
Liquid-solid and liquid-liquid phase separation (PS) drives the formation of functional and disease-associated biological assemblies. Principles of phase equilibrium are here employed to derive a general kinetic solution that predicts the evolution of the mass and size of biological assemblies. Thermodynamically, protein PS is determined by two measurable concentration limits: the saturation concentration and the critical solubility. Due to surface tension effects, the critical solubility can be higher than the saturation concentration for small, curved nuclei. Kinetically, PS is characterized by the primary nucleation rate constant and a combined rate constant accounting for growth and secondary nucleation. It is demonstrated that the formation of a limited number of large condensates is possible without active mechanisms of size control and in the absence of coalescence phenomena. The exact analytical solution can be used to interrogate how the elementary steps of PS are affected by candidate drugs.
Identifiants
pubmed: 37279376
doi: 10.1002/advs.202301501
pmc: PMC10427409
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2301501Subventions
Organisme : European Union's Horizon
ID : 952334
Organisme : FEDER-Fundo Europeu de Desenvolvimento Regional
Organisme : COMPETE 2020-Operational Programme for Competitiveness and Internationalization
Organisme : Portugal 2020
ID : PTDC/QUI-COL/2444/2021
Organisme : Portugal 2020
ID : POCI-01-0145-FEDER-031323
Organisme : Portugal 2020
ID : PTDC/MED-FAR/31323/2017
Organisme : Portugal 2020
ID : POCI-01-0145-FEDER-007274
Organisme : Institute for Research and Innovation in Health Sciences
ID : UIDB/00511/2020
Organisme : Laboratory for Process Engineering, Environment
Organisme : Biotechnology and Energy
ID : 2021/43/B/ST8/01900
Organisme : National Science Center, Poland
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
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