Drug screening at single-organoid resolution via bioprinting and interferometry.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 06 2023
06 06 2023
Historique:
received:
02
08
2022
accepted:
17
05
2023
medline:
8
6
2023
pubmed:
7
6
2023
entrez:
6
6
2023
Statut:
epublish
Résumé
High throughput drug screening is an established approach to investigate tumor biology and identify therapeutic leads. Traditional platforms use two-dimensional cultures which do not accurately reflect the biology of human tumors. More clinically relevant model systems such as three-dimensional tumor organoids can be difficult to scale and screen. Manually seeded organoids coupled to destructive endpoint assays allow for the characterization of treatment response, but do not capture transitory changes and intra-sample heterogeneity underlying clinically observed resistance to therapy. We present a pipeline to generate bioprinted tumor organoids linked to label-free, time-resolved imaging via high-speed live cell interferometry (HSLCI) and machine learning-based quantitation of individual organoids. Bioprinting cells gives rise to 3D structures with unaltered tumor histology and gene expression profiles. HSLCI imaging in tandem with machine learning-based segmentation and classification tools enables accurate, label-free parallel mass measurements for thousands of organoids. We demonstrate that this strategy identifies organoids transiently or persistently sensitive or resistant to specific therapies, information that could be used to guide rapid therapy selection.
Identifiants
pubmed: 37280220
doi: 10.1038/s41467-023-38832-8
pii: 10.1038/s41467-023-38832-8
pmc: PMC10244450
doi:
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3168Subventions
Organisme : NCI NIH HHS
ID : R21 CA227480
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM073981
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA185189
Pays : United States
Organisme : NCI NIH HHS
ID : U24 CA248265
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA244729
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM127985
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM114188
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA016042
Pays : United States
Informations de copyright
© 2023. The Author(s).
Références
Nat Mater. 2021 Jan;20(1):22-29
pubmed: 32958879
Mol Oncol. 2007 Jun;1(1):84-96
pubmed: 18516279
Methods. 2017 Feb 15;115:80-90
pubmed: 27713081
Adv Pharmacol. 2012;65:335-59
pubmed: 22959031
Nat Cancer. 2020 Aug;1(8):761-773
pubmed: 34142093
Arch Med Sci. 2018 Jun;14(4):910-919
pubmed: 30002710
Blood Rev. 2017 Nov;31(6):418-425
pubmed: 28797519
Nature. 1952 Mar 29;169(4300):541
pubmed: 14929230
Br J Clin Pharmacol. 2017 Mar;83(3):554-561
pubmed: 27628584
Biomed Opt Express. 2019 Jun 26;10(7):3605-3621
pubmed: 31360607
Front Pharmacol. 2018 Jan 23;9:6
pubmed: 29410625
Commun Biol. 2022 Aug 8;5(1):794
pubmed: 35941353
Nature. 1953 Apr 25;171(4356):720-4
pubmed: 13054689
PLoS One. 2014 Dec 22;9(12):e115726
pubmed: 25531652
Front Mol Biosci. 2020 Mar 06;7:33
pubmed: 32211418
Br J Cancer. 2020 Oct;123(8):1209-1218
pubmed: 32728094
Int J Mol Sci. 2012;13(1):427-52
pubmed: 22312262
Nat Protoc. 2020 Mar;15(3):1098-1131
pubmed: 31996844
Biotechnol J. 2009 Aug;4(8):1168-77
pubmed: 19507149
ACS Nano. 2008 May;2(5):841-6
pubmed: 19206480
Cancer Discov. 2022 Feb;12(2):388-401
pubmed: 34789538
Biotechnol Adv. 2017 Mar - Apr;35(2):217-239
pubmed: 28057483
Biomed Opt Express. 2021 Jun 03;12(7):3789-3805
pubmed: 34457380
ACS Omega. 2018 Dec 31;3(12):17687-17692
pubmed: 30613814
Biofabrication. 2020 Jul 09;12(4):042001
pubmed: 32470967
Front Med (Lausanne). 2018 Apr 06;5:85
pubmed: 29682505
Sci Adv. 2022 Feb 18;8(7):eabl3674
pubmed: 35171675
J Cancer. 2017 Sep 12;8(16):3131-3141
pubmed: 29158785
BMC Bioinformatics. 2019 Jan 21;20(1):42
pubmed: 30665349
Pharmacol Ther. 2021 Feb;218:107668
pubmed: 32853629
Appl Phys Rev. 2019 Mar;6(1):
pubmed: 33738018
SLAS Technol. 2018 Oct;23(5):432-439
pubmed: 30045649
Cell. 2021 Jan 7;184(1):18-32
pubmed: 33417859
Nature. 1954 May 1;173(4409):821-2
pubmed: 13165653
Trends Mol Med. 2017 Oct;23(10):874-898
pubmed: 28887051
Cancer Cell. 2021 Sep 13;39(9):1190-1201
pubmed: 34416168
Bioinformatics. 2018 Sep 1;34(17):i884-i890
pubmed: 30423086
J Physiol. 1952 Oct;118(2):38P-39P
pubmed: 13000745
CA Cancer J Clin. 2020 Mar;70(2):125-137
pubmed: 32031692
Analyst. 2021 Feb 21;146(4):1157-1162
pubmed: 33426547
Cytometry A. 2021 Oct;99(10):1022-1032
pubmed: 33305901
Biophys J. 2011 Sep 7;101(5):1025-31
pubmed: 21889438
Cancer Discov. 2022 Feb;12(2):372-387
pubmed: 34635570
Anticancer Res. 2015 Jun;35(6):3147-54
pubmed: 26026074
Biochem Biophys Res Commun. 1986 Mar 13;135(2):397-402
pubmed: 3457562
SLAS Discov. 2021 Oct;26(9):1107-1124
pubmed: 34111999
Cell Rep Med. 2020 Dec 22;1(9):100161
pubmed: 33377132
Annu Rev Pathol. 2011;6:95-119
pubmed: 20936938
J 3D Print Med. 2019 Jun;3(2):55-58
pubmed: 31258934
Annu Rev Pathol. 2020 Jan 24;15:211-234
pubmed: 31550983
Cell. 2015 Sep 24;163(1):39-53
pubmed: 26406370
Expert Rev Neurother. 2015;15(7):741-52
pubmed: 26027432
Am Soc Clin Oncol Educ Book. 2017;37:160-169
pubmed: 28561651
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Nat Cancer. 2022 Feb;3(2):232-250
pubmed: 35221336
Cell. 2018 May 3;173(4):1003-1013.e15
pubmed: 29681457
Clin Cancer Res. 2017 Jul 15;23(14):3489-3498
pubmed: 28364015
PLoS One. 2014 Feb 18;9(2):e89000
pubmed: 24558461
Semin Cancer Biol. 2005 Oct;15(5):405-12
pubmed: 16055341
Cell. 2021 Apr 15;184(8):2239-2254.e39
pubmed: 33831375
ACS Appl Bio Mater. 2020 Sep 21;3(9):5552-5573
pubmed: 35021789
Cancer Discov. 2018 Sep;8(9):1112-1129
pubmed: 29853643
Analyst. 2019 Dec 16;145(1):97-106
pubmed: 31746831
Nat Rev Cancer. 2018 Jul;18(7):407-418
pubmed: 29692415
Philos Trans R Soc Lond B Biol Sci. 2017 Feb 5;372(1713):
pubmed: 27994117
Drugs Today (Barc). 2006 Jul;42(7):441-53
pubmed: 16894399
J Cell Biol. 2017 Jan 2;216(1):31-40
pubmed: 28031422
Sci Signal. 2020 Jun 16;13(636):
pubmed: 32546544
Invest New Drugs. 2021 Feb;39(1):77-88
pubmed: 32833135
J Natl Cancer Inst. 2015 Mar 31;107(7):
pubmed: 25828948
Cell. 2018 Jan 11;172(1-2):373-386.e10
pubmed: 29224780
Nat Mater. 2021 Feb;20(2):260-271
pubmed: 33230326
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
BMC Bioinformatics. 2016 Oct 21;17(1):428
pubmed: 27769170
STAR Protoc. 2020 Sep 18;1(2):
pubmed: 33043307
Cell Rep. 2017 Aug 29;20(9):2215-2226
pubmed: 28854369
Biofabrication. 2019 Jan 24;11(2):025003
pubmed: 30616234
J Cancer Res Clin Oncol. 2002 Mar;128(3):125-34
pubmed: 11935298
Essays Biochem. 2021 Aug 10;65(3):603-610
pubmed: 34028520
Curr Opin Oncol. 2016 Sep;28(5):441-6
pubmed: 27428363
Commun Biol. 2019 Feb 25;2:78
pubmed: 30820473
Drug Resist Updat. 2010 Aug-Oct;13(4-5):99-108
pubmed: 20739212
Breast Cancer Res. 2014 May 20;16(3):210
pubmed: 25928070
PLoS Comput Biol. 2022 Nov 9;18(11):e1010584
pubmed: 36350878
Nucleic Acids Res. 2018 Apr 20;46(7):3671-3691
pubmed: 29385530
J Clin Oncol. 2020 Nov 20;38(33):3883-3894
pubmed: 33048619
Anal Chem. 2018 Mar 6;90(5):3299-3306
pubmed: 29381859
Sci Rep. 2016 Jul 01;6:28951
pubmed: 27364600
Breast Cancer (Dove Med Press). 2015 Jun 09;7:147-62
pubmed: 26089701
Opt Express. 2009 Jul 20;17(15):13080-94
pubmed: 19654713
Science. 2019 Jun 07;364(6444):952-955
pubmed: 31171691
Am J Physiol Cell Physiol. 2008 Aug;295(2):C538-44
pubmed: 18562484
Nat Methods. 2014 Dec;11(12):1221-8
pubmed: 25423019
Proc Natl Acad Sci U S A. 2014 Dec 23;111(51):E5593-601
pubmed: 25480548
ACS Nano. 2022 Aug 23;16(8):11516-11544
pubmed: 35916417
Cell Rep. 2017 Aug 1;20(5):1229-1241
pubmed: 28768205
Nat Med. 2017 Sep 8;23(9):1028-1035
pubmed: 28886003
SLAS Discov. 2020 Aug;25(7):709-722
pubmed: 32484408
Nanotechnology. 2008 Jun 11;19(23):235101
pubmed: 20737027
EMBO J. 2019 Aug 1;38(15):e101654
pubmed: 31282586
Nat Methods. 2022 Jul;19(7):829-832
pubmed: 35654950
Analyst. 2012 Dec 7;137(23):5495-8
pubmed: 23057068
Transl Oncol. 2021 Aug;14(8):101126
pubmed: 34020369
Science. 2018 Feb 23;359(6378):920-926
pubmed: 29472484
Biophys J. 2011 May 4;100(9):2309-17
pubmed: 21539801
Nature. 1952 Mar 1;169(4296):366-7
pubmed: 14919571
Clin Cancer Res. 2017 Aug 15;23(16):4680-4692
pubmed: 28446504
Drug Des Devel Ther. 2014 Oct 14;8:1911-21
pubmed: 25342884