Microscope-Cockpit: Python-based bespoke microscopy for bio-medical science.
Adaptive optics (AO)
Artificial Intelligence (AI)
Bespoke microscope
Free and open source software
Imaging
Machine Learning (ML)
Microscope hardware device control
Microscope-Python
Super resolution microscopy
Journal
Wellcome open research
ISSN: 2398-502X
Titre abrégé: Wellcome Open Res
Pays: England
ID NLM: 101696457
Informations de publication
Date de publication:
2021
2021
Historique:
accepted:
04
01
2022
pubmed:
8
4
2021
medline:
8
4
2021
entrez:
7
6
2023
Statut:
epublish
Résumé
We have developed "Microscope-Cockpit" (Cockpit), a highly adaptable open source user-friendly Python-based Graphical User Interface (GUI) environment for precision control of both simple and elaborate bespoke microscope systems. The user environment allows next-generation near instantaneous navigation of the entire slide landscape for efficient selection of specimens of interest and automated acquisition without the use of eyepieces. Cockpit uses "Python-Microscope" (Microscope) for high-performance coordinated control of a wide range of hardware devices using open source software. Microscope also controls complex hardware devices such as deformable mirrors for aberration correction and spatial light modulators for structured illumination via abstracted device models. We demonstrate the advantages of the Cockpit platform using several bespoke microscopes, including a simple widefield system and a complex system with adaptive optics and structured illumination. A key strength of Cockpit is its use of Python, which means that any microscope built with Cockpit is ready for future customisation by simply adding new libraries, for example machine learning algorithms to enable automated microscopy decision making while imaging.
Identifiants
pubmed: 37283605
doi: 10.12688/wellcomeopenres.16610.1
pmc: PMC10240544
doi:
Types de publication
Journal Article
Langues
eng
Pagination
76Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
Copyright: © 2022 Phillips MA et al.
Déclaration de conflit d'intérêts
Competing interests: Martin Booth declares a significant interest in Aurox Ltd., whose microscopes were used in this work.
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