Derivation of the small-angle scattering profile of a target biomacromolecule from a profile deteriorated by aggregates. AUC-SAS.
aggregates
analytical ultracentrifugation
biomacromolecules
protein solutions
small-angle X-ray scattering
small-angle neutron scattering
Journal
Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190
Informations de publication
Date de publication:
01 Jun 2023
01 Jun 2023
Historique:
received:
07
10
2022
accepted:
12
03
2023
medline:
7
6
2023
pubmed:
7
6
2023
entrez:
7
6
2023
Statut:
epublish
Résumé
Aggregates cause a fatal problem in the structural analysis of a biomacro-mol-ecule in solution using small-angle X-ray or neutron scattering (SAS): they deteriorate the scattering profile of the target molecule and lead to an incorrect structure. Recently, an integrated method of analytical ultracentrifugation (AUC) and SAS, abbreviated AUC-SAS, was developed as a new approach to overcome this problem. However, the original version of AUC-SAS does not offer a correct scattering profile of the target molecule when the weight fraction of aggregates is higher than
Identifiants
pubmed: 37284265
doi: 10.1107/S1600576723002406
pii: S1600576723002406
pmc: PMC10241049
doi:
Types de publication
Journal Article
Langues
eng
Pagination
624-632Informations de copyright
© Ken Morishima et al. 2023.
Références
Biophys J. 2020 May 5;118(9):2209-2219
pubmed: 31952809
Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1278-89
pubmed: 22993082
Science. 2014 Dec 12;346(6215):1377-80
pubmed: 25504723
Protein Sci. 2002 Sep;11(9):2067-79
pubmed: 12192063
Commun Biol. 2022 Mar 10;5(1):184
pubmed: 35273347
Biochemistry. 2006 Mar 14;45(10):3146-53
pubmed: 16519509
Elife. 2020 Jul 30;9:
pubmed: 32729831
Nat Methods. 2018 Mar;15(3):191-193
pubmed: 29377013
Biochim Biophys Acta Gen Subj. 2018 Feb;1862(2):253-274
pubmed: 29107147
J Mol Biol. 2020 Apr 17;432(9):3078-3092
pubmed: 32035901
Bioinformatics. 2014 Jun 15;30(12):1771-3
pubmed: 24532726
Sci Rep. 2019 Aug 30;9(1):12610
pubmed: 31471544
J Biomol Struct Dyn. 2019 Aug;37(12):3058-3064
pubmed: 30582420
Acta Crystallogr D Struct Biol. 2018 Aug 1;74(Pt 8):715-726
pubmed: 30082507
Acta Crystallogr D Struct Biol. 2018 Dec 1;74(Pt 12):1178-1191
pubmed: 30605132
Biophys J. 2000 Mar;78(3):1606-19
pubmed: 10692345
J Appl Crystallogr. 2018 Nov 09;51(Pt 6):1623-1632
pubmed: 30546289
Biophys J. 2006 Jun 15;90(12):4651-61
pubmed: 16565040
Sci Rep. 2022 Jun 15;12(1):9970
pubmed: 35705644
J Biochem. 2021 Sep 7;169(6):701-708
pubmed: 33585933
Sci Rep. 2021 Mar 11;11(1):5655
pubmed: 33707747
Biophys Chem. 2001 Nov 28;93(2-3):129-39
pubmed: 11804721
J Appl Crystallogr. 2021 Feb 01;54(Pt 1):343-355
pubmed: 33833657
J Appl Crystallogr. 2016 Nov 02;49(Pt 6):2015-2020
pubmed: 27980509
Commun Biol. 2020 Jun 8;3(1):294
pubmed: 32513995
J Appl Crystallogr. 2012 Mar 15;45(Pt 2):342-350
pubmed: 25484842
Commun Biol. 2021 Feb 12;4(1):191
pubmed: 33580188